고능력 한우의 체내수정란 생산효율과 수정란이식
Efficiency of in vivo Embryo Production in Elite Hanwoo and Embryo Transfer
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The study was conducted to investigate the comparison of pregnancy rate and transferable embryos produced by genetically superior Korean cows (Hanwoo) of livestock farms. Eighteen Hanwoo donors were superovulated with gonadotropin for 4 days combined with Progesterone Releasing Intravaginal. Embryos were recovered 7 days after the second insemination by flushing the uterus with embryo collection medium. No differences were observed in the efficiency of rate of superovulation in groups A (low nutrition) and B (highnutrition) it was observed to be 100.0% and 87.5%, respectively. The mean numbers of total embryos were 10.8±3.4 and 8.9±2.5, and transferable embryos were 7.5±3.3 and 4.0±1.5 in groups A and B, respectively. The pregnancy rates after embryo transfer were 23.5%, 20.0%, C 80.0% and 55.6% in farm A, B, C, and D, respectively. In conclusion, results suggest that superovulation could be used quite effectively to raise superior Hanwooembryos. However, physical and biological condition of recipients greatly affects the rate of pregnancy.
Abstract
The study was conducted to investigate the comparison of pregnancy rate and transferable embryos produced by genetically superior Korean cows (Hanwoo) of livestock farms. Eighteen Hanwoo donors were superovulated with gonadotropin for 4 days combined with Progesterone Releasing Intravaginal. Embryos were recovered 7 days after the second insemination by flushing the uterus with embryo collection medium. No differences were observed in the efficiency of rate of superovulation in groups A (low nutrition) and B (highnutrition) it was observed to be 100.0% and 87.5%, respectively. The mean numbers of total embryos were 10.8±3.4 and 8.9±2.5, and transferable embryos were 7.5±3.3 and 4.0±1.5 in groups A and B, respectively. The pregnancy rates after embryo transfer were 23.5%, 20.0%, C 80.0% and 55.6% in farm A, B, C, and D, respectively. In conclusion, results suggest that superovulation could be used quite effectively to raise superior Hanwooembryos. However, physical and biological condition of recipients greatly affects the rate of pregnancy.
참고문헌 (25)
- Bastidas, P., "Seasonal effects on embryo transfer results in Brahman cows", Theriogenology, 28, pp.531~540 (1987)
- 조상래, "다배란 처리에 따른 한우 체내 수정란 생산 효율과 수정란이식", 한국수정란이식학회지, 25(3), pp.141~144 (2010), 한국수정란이식학회
- Christensen, L. G., "Use of embryo transfer in future cattle breeding schemes", Theriogenology, 35, pp.141~149 (1991)
- Donaldson, L. E., "Effect of age of donor cows on embryo production", Theriogenology, 21, pp.963~967 (1984)
- Donaldson, L. E., "Embryo production by repeated superovulation of commercial donor cows", Theriogenology, 20, pp.163~168 (1983)
- Duncan, D. B., "Multiple range and multiple F-tests", Biometrics, 11, pp.1~42 (1995)
- Ginther, O. G., "Temporal associations among ovarian events in cattle during oestrous cycles with two and three follicular waves", J. Reprod. Frt, 87, pp.223~230 (1989)
- Hasler, J. F., "The relationship between age and response to superovulation in Holstein cows and heifers", Theriogenology, 15, pp.109~ (1981)
- Isogai, T., "Factors affecting embryo production following repeated superovulation treatment in Holstein donors", J. Reprod. Dev, 39, pp.79~84 (1993)
- Lindener, G. M., "Bovine embryo morphology and evaluation", Theriogenology, 20, pp.407~413 (1983)
- Markusfeld, O., "Body condition score, health, yield and fertility in dairy cows", Vet. Rec, 141, pp.67~72 (1997)
- Nolan, R., "Callaghan, D. O., Duby, R. T., Lonergan, P. and Boland, M. P. 1998. The influence of short-term nutrient changes on follicle growth and embryo production following superovulation in beef heifers", Theriogenology, 50, pp.1263~1274 (1998)
- 박해금, "과배란 처리에 따른 한우와 칡소 체내 수정란 생산 효율과 수정란이식 수태율", Reproductive & Developmental biology, 36(3), pp.231~235 (2012), 한국동물번식학회
- Remsen, L. G., "Pregnancy rates relating to plasma progesterone levels in recipient heifers at day of transfer", Theriogenology, 18, pp.365~372 (1982)
- Seidel, G. E., "Superovulation and embryo transfer in cattle", Science, 211, pp.351~358 (1981)
- Selk, G. E., "Relationship among weight change, body condition and reproductive performance of range beef cows", J. Anim. Sci, 66, pp.3153~3159 (1988)
- Shea, B. F., "variation in response to stimulation and related embryo transfer procedures in Alberta over a nine year period", Theriogenology, 21, pp.186~195 (1984)
- Smith, C., "Genetic improvemet of livestock, using nucleus breeding units", World Animal Review, 65, pp.2~10 (1984)
- Smith, C., "Application of embryo transfer in animal breeding", Theriogenology, pp.203~212 (1988)
- 손동수, "우수 한우의 수정란 생산 및 이식", 한국수정란이식학회지, 21(2), pp.147~156 (2006), 한국수정란이식학회
- Son, D. S., "Embryo production and transfer for dairy MOET scheme application", J. Emb. Trans, 26, pp.57~65 (2000)
- Stringfellow, D. A., "Manual of the International Embryo Transfer Society", pp.165~170 (1998), International Embryo Transfer Society, Inc
- Stubbings, R. B., "Relationship between plasma progesterone concentrations and pregnancy rates in cattle receiving either fresh of previously frozen embryos", Theroigenology, 26, pp.145~155 (1986)
- Takedomi, T., "Superovulation in Holstein heifers by a single injection of porcine FSH dissolved in polyvinylpyrrolidone", Theriogenology, 39, pp.327~ (1992)
- Yamamoto, M., "Superovulation in the cow with a single intramuscular injection of FSH dissolved in polyvinylpyrrolidone", Theriogenology, 41, pp.747~755 (1994)
