한우 공란우 및 생체내 난자 회수(ovum pick-up) 조건이 체외수 정란의 발달에 미치는 효과
Effects of donors and in vivo ovum pick-up conditions on in vitro embryo development in Korean native cow
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Artificial insemination of Korean native cattle (KNC) is the predominant method for breed improve-ment. However, industrialization of embryo production and transfer is necessary to utilize the ge-netic potential of KNC. The aim of this study was to examine associations between KNC donor cows and ovum pick-up (OPU) conditions, in-vivo oocyte recovery, and embryo development. Oocyte recovery and blastocyst development rates were higher at 50 and 60 mmHg OPU vacuum pressure than at 40 mmHg, which was, however, not significant. Regarding follicle growth, injection of 500 mg GnRH 36 hours before OPU significantly increased the number of OPU oocytes from an average of 4.6 to 7.6 (P <0.05); no significant difference in embryo development rates was observed. Significant differences were observed in the numbers of OPU oocytes, embryo development rates, and trans-plantable blastocysts per individual among nine KNC donors (P <0.05). Furthermore, although there was no difference in OPU oocyte recovery intervals in approximately 2∼8 weeks, the number of recovered oocytes significantly decreased at the 12-week interval (P <0.05); there was no difference in embryo development rates. The number of oocytes and embryonic development rates only tended to decrease until the seventh OPU session, but decreased significantly until the eighth session (P <0.05). The average pregnancy rate after transfer of OPU-derived in-vitro embryos into recipient cows was 41.8%. To improve the efficiency of OPU egg recovery and in-vitro embryo production, considering KNC donor characteristics, vacuum pressure of 60 mmHg, GnRH pretreatment to induce follicle growth, and effective OPU egg recovery up to seven times at intervals of 2∼4 weeks appears to be most suitable. This study may facilitate the industrialization of KNC embryo production and transfer using high-quality cows.
Abstract
Artificial insemination of Korean native cattle (KNC) is the predominant method for breed improve-ment. However, industrialization of embryo production and transfer is necessary to utilize the ge-netic potential of KNC. The aim of this study was to examine associations between KNC donor cows and ovum pick-up (OPU) conditions, in-vivo oocyte recovery, and embryo development. Oocyte recovery and blastocyst development rates were higher at 50 and 60 mmHg OPU vacuum pressure than at 40 mmHg, which was, however, not significant. Regarding follicle growth, injection of 500 mg GnRH 36 hours before OPU significantly increased the number of OPU oocytes from an average of 4.6 to 7.6 (P <0.05); no significant difference in embryo development rates was observed. Significant differences were observed in the numbers of OPU oocytes, embryo development rates, and trans-plantable blastocysts per individual among nine KNC donors (P <0.05). Furthermore, although there was no difference in OPU oocyte recovery intervals in approximately 2∼8 weeks, the number of recovered oocytes significantly decreased at the 12-week interval (P <0.05); there was no difference in embryo development rates. The number of oocytes and embryonic development rates only tended to decrease until the seventh OPU session, but decreased significantly until the eighth session (P <0.05). The average pregnancy rate after transfer of OPU-derived in-vitro embryos into recipient cows was 41.8%. To improve the efficiency of OPU egg recovery and in-vitro embryo production, considering KNC donor characteristics, vacuum pressure of 60 mmHg, GnRH pretreatment to induce follicle growth, and effective OPU egg recovery up to seven times at intervals of 2∼4 weeks appears to be most suitable. This study may facilitate the industrialization of KNC embryo production and transfer using high-quality cows.
참고문헌 (51)
- 농림축산식품부, "정액증명서등", 축산법 제18조, ~ (2020)
- Blondin P, "Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes", Mol. Reprod. Dev, 41, pp.54~62 (1995)
- Bols PEJ, "Effect of needle tip bevel and aspiration procedure on the morphology and developmental capacity of bovine compact cumulus oocytes complexes", Theriogenology, 46, pp.899~909 (1997)
- Chastant-Maillard S, "Consequences of transvaginal follicular puncture on well-being in cows", Reproduction, 125, pp.555~563 (2003)
- Chaubal SA, "Hormonal treatments for increasing the oocyte and embryo production in an OPU-IVP system", Theriogenology, 67, pp.719~728 (2007)
- Chaubal SA, "Comparison of different transvaginal ovum pickup protocols to optimise oocyte retrieval and embryo production over at 10-week period in cows", Theriogenology, 65, pp.1631~1648 (2006)
- 조상래, "한우의 수정란이식 효율성 향상을 위한 생체난포란 채취에 관한 연구", 한국동물생명공학회지, 32(3), pp.147~151 (2017), 사단법인 한국동물생명공학회
- De Roover R, "Effects of ovum pick-up frequency and FSH stimulation : a retrospective study on seven years of beef cattle in vitro embryo production", Reprod. Domest. Anim, 43, pp.239~245 (2008)
- De Roover R, "Ovum pick up and in vitro embryo production in cows superstimulated with an individually adapted superstimulation protocol", Ani. Reprod. Sci, 86, pp.13~25 (2005)
- "Statistical information committee report (2019 DATA)" (2020)[URL]
- Galli C, "Embryo production by ovum pick up from live donors", Theriogenology, 55, pp.1341~1357 (2001)
- Galli C, "Ovum pick up, intracytoplasmic sperm injection and somatic cell nuclear transfer in cattle, buffalo and horses : from the research laboratory to clinical practice", Theriogenology, 81, pp.138~151 (2014)
- Galli C, "Embryo production by ovum pick up from live donors", Theriogenology, 55, pp.1341~1357 (2000)
- Hasler JF, "Production, freezing and transfer of bovine IVF embryos and subsequent calving results", Theriogenology, 43, pp.141~152 (1995)
- Hasler JF, "Factors affecting frozen and fresh embryo transfer pregnancy rates in cattle", Theriogenology, 56, pp.1401~1415 (2001)
- Hasler JF, "Forty years of embryo transfer in cattle : A review focusing on the journal Theriogenology, the growth of the industry in North America, and personal reminisces", Theriogenology, 81, pp.152~169 (2014)
- Hawk HW, "Improved yield of bovine blastocysts from in vitro produced oocytes selection of oocytes and zygotes", Theriogenology, 41, pp.1571~1583 (1995)
- Hendriksen PJM, "Bovine follicular development and its effect on the in vitro competence of oocytes", Theriogenology, 53, pp.11~20 (2000)
- 진종인, "OPU 유래 한우 수정란 생산 및 이식", 한국동물생명공학회지, 29(3), pp.273~281 (2014), 사단법인 한국동물생명공학회
- 진종인, "OPU 유래 수정란의 대량생산을 위한 고능력 공란우 반복사용 가능성에 관한 연구", 한국동물생명공학회지, 30(3), pp.149~159 (2015), 사단법인 한국동물생명공학회
- 진종인, "OPU(Ovum Pick-Up) 채란기간이 난자 및 수정란 생산에 미치는 영향", 한국동물생명공학회지, 25(1), pp.15~20 (2010), 사단법인 한국동물생명공학회
- Kim SS, "Associations between pedigree, meat quality, and meat quantity of slaughterhouse donor cows and oocyte recovery, embryo development, and pregnancy after embryo transfer", Livestock Science, 142, pp.42~47 (2011)
- Leroy JL, "Reduced fertility in high-yielding dairy cows: are the oocyte and embryo in danger? Part I. The importance of negative energy balance and altered corpus luteum function to the reduction of oocyte and embryo quality in high-yielding dairy cows", Reprod. Domest. Anim, 43, pp.612~622 (2008)
- Leroy JL, "Intrafollicular conditions as a major link between maternal metabolism and oocyte quality : a focus on dairy cow fertility", Reprod. Fertil. Dev, 24, pp.1~12 (2011)
- Leroy JL, "Reduced fertility in high-yielding dairy cows: are the oocyte and embryo in danger? Part II. Mechanisms linking nutrition and reduced oocyte and embryo quality in high-yielding dairy cows", Reprod. Domest. Anim, 43, pp.623~632 (2008)
- Leroy JL, "Non-esterified fatty acids in follicular fluid of dairy cows and their effect on developmental capacity of bovine oocytes in vitro", Reproduction, 130, pp.485~495 (2005)
- Lonergan P, "Effect of culture environment on gene expression and develop mental characteristics in IVF-derived embryos", Theriogenology, 65, pp.137~152 (2006)
- Machatkova M, "Developmental competence of bovine oocytes : effects of follicle size and the phase of follicular wave on in vitro embryo production", Theriogenology, 61, pp.329~335 (2004)
- Matoba S, "Optimizing production of in vivo-matured oocytes from superstimulated-Holstein cows for in vitro production of embryos using X-sorted sperm", J. Dairy Sci, 97, pp.743~753 (2014)
- Merton JS, "Genetic parameters for oocyte number and embryo production within a bovine ovum pick-up-in vitro production embryoproduction program", Theriogenology, 72, pp.885~893 (2009)
- Merton JS, "Factors affecting oocyte quality and quantity in commercial application of embryo technologies in the cattle breeding industry", Theriogenology, 59, pp.651~674 (2003)
- Nasser LF, "Exogenous progesterone enhances ova and embryo quality following superstimulation of the first follicular wave in Nelore(Bos indicus)donors", Theriogenology, 76, pp.320~327 (2011)
- Nolan R, "The influence of short-term nutrient changes on follicle growth and embryo production following superovulation in beef heifers", Theriogenology, 50, pp.1263~1274 (1998)
- Numabe T, "Production efficiency of Japanese black calves by transfer of bovine embryos produced in vitro", Theriogenology, 54, pp.1409~1420 (2000)
- Park SJ, "The study of factor concentrating ovum pick-up and conception rate by ultrasound-guided follicular aspiration in hanwoo heifers", Korean J. Animal Reprod, 24, pp.199~208 (2000)
- Park SJ, "Studies on the ultrasound-guided transvaginal retrieval of oocytes in korean native, hanwoo heifers Ⅰ. Characteristics of hanwoo ovary during the estrous cycle", Korean J. Animal. Reprod, 24, pp.77~82 (2000)
- Park SJ, "Studies on the ultrasound-guided transvaginal retrieval of oocytes in korean native, hanwoo heifers Ⅱ. Effects of repeated ovum pick-up on oocyte recovery and estrous cycle", Korean J. Animal. Reprod, 24, pp.83~88 (2000)
- Pieterse MC, "Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries", Theriogenology, 30, pp.751~762 (1988)
- Pursely JR, "Impact of circulating concentrations of progesterone and antral age of the ovulatory follicle on fertility of high-producing lactating dairy cows", Reprod. Fertil. Dev, 24, pp.267~271 (2011)
- Rivera FA, "Reduced progesterone concentration during growth of the first follicular wave affects embryo quality but has no effect on embryo survival post transfer in lactating dairy cows", Reproduction, 141, pp.333~342 (2011)
- Rizos D, "Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro verses in vivo : Implications for blastocyst yield and blastocyst quality", Mol. Reprod. Dev, 61, pp.234~248 (2002)
- Sakaguchi K, "Effects of follicle-stimulating hormone followed by gonadotropin-releasing hormone on embryo production by ovum pick-up and in vitro fertilization in the river buffalo(Bubalus bubalis)", Anim. Sci. J, 90, pp.690~695 (2019)
- Santos JEP, "Nutritional management of the donor cow", Theriogenology, 69, pp.88~97 (2008)
- Scott CA, "Technical aspects of transvaginal ultrasound-guided follicullar aspiration in cows", Vet. Rec, 134, pp.440~443 (1994)
- Snijders SEM, "Effect of genetic merit, milk yield, body condition and lactation number on in vitro oocyte development in dairy cows", Theriogenology, 53, pp.981~989 (2000)
- Staigmiller RB, "Effect of nutrition on response to exogenous FSH in beef cattle", J. Anim. Sci, 48, pp.1182~1190 (1979)
- Su L, "Effect of donor age on the developmental competence of bovine ooeytes retrievd by ovum pick up", Reprod. Dom. Anim, 47, pp.184~189 (2012)
- Sunderland SJ, "Selection, dominance and atresia of follicles during the oestrous cycle of heifers", J. Reprod. Fertil, 101, pp.547~555 (1994)
- Van Wagtendonk-de Leeuw AM, "Effects of different reproduction techniques : AI, MOET or IVP, on health and welfare of bovine offspring", Theriogenology, 53, pp.575~597 (2000)
- Ward FA, "Factors affecting recovery and quality of oocytes for bovine embryo production in vitro using ovum pick-up technology", Theriogenology, 54, pp.433~446 (2000)
- Yaakub H, "Effect of type and quantity of concentrates on superovulation and embryo yield in beef heifers", Theriogenology, 51, pp.1259~1266 (1999)