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Protective Effects of Phenolic-rich Fraction(PRF) from Fructus Schisandrae on H2O2-induced Apoptosis of SH-SY5Y Cells

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Abstract

Objective : This study was intended to ascertain the protective effect of phenolic-rich fraction (PRF) from Fructus Schisandrae on SH-SY5Y cells.Methods : PRF was obtained from the 80% ethanol extract of Fructus Schisandrae by Sepabeads SP-850 column chromatography. The neuroprotective effect of the FS PRF was investigated due to the hydrogen peroxide (H2O2)-induced apoptosis of cultured SH-SY5Y cells.Results : Cell viability assays revealed that pretreating SH-SY5Y cells with PRF (10-200 g/mL) resulted in significant dose-dependent protection against H2O2-induced cell death. The effect was assessed by flow cytometric analysis of DNA contents using propidium iodide (PI) staining. The population of apoptotic cells was increased by 32.89% in only H2O2 (150 M)-treated environment, but it was reduced by pre-treatment of FS PRF (200 g/mL) to 21.61%. H2O2-induced caspase-3 activation and PARP cleavage were reduced in FS PRF pre-treated cells, and PRF led to an apparent suppressive effect on the oxidative stress induced by reactive oxygen species (ROS).Conclusion : This study showed that Fructus Schisandrae should be useful for the treatment and prevention of neurodegenerative diseases associated with elevated ROS levels.

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