산소-포도당 결핍(OGD) 유도성 신경세포 사멸에 대한 뇌 보호 효과를 가지는 수종 생약추출물의 검색
Neuroprotective Effects of Some Plant Extracts against Oxygen-Glucose Deprivation (OGD)-Induced Oxidative Cell Death on Neuronal Cell
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Abstract
Cerebral ischemia results from a transient or permanent reduction in cerebral blood flow that decreases oxygen and glucose supply. When the cellular oxygen supply is reduced to critical level, damage to cells and induction of cell death are occurred by excitotoxicity, oxidative stress and inflammation. Ischemia remains one of the leading causes of death, but there is no effective treatment that might protect neurons against ischemia by interrupting the cascade of cell death. In this study, human neuroblastoma SH-SY5Y cells are exposed to oxygen and glucose deprivation (OGD) followed by reoxgenation. OGD can mimic the acute restriction of metabolite and oxygen supply caused by ischemia and is widely used as a model of ischemic conditions. SH-SY5Y cells are treated samples at the commencement of OGD to achieve different final concentrations, and cell viabilities were quantified using the measurement of flow cytometry analysis. Of those tested, the extracts of Polygala tenuifolia (roots), Dictamnus dasycarpus (barks), Polygala tenuifolia (roots), Eucommia ulmoides (branches), Eucommia ulmoides (barks), Poria cocos (whole), Sophora flavescens (roots) showed neuroprotective effects, with EC50 values of 4.5 ± 0.6, 7.9 ± 1.5, 10.5 ± 0.7, 18.4 ± 1.9, 19.6 ± 0.3, 21.6 ± 1.9 and 30.7 ± 3.9㎍/㎖, respectively.
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