수종 지혈 한약물이 Cytochrome P450 3A4 활성에 미치는 영향
Influence of Five Herbal Medicines on Cytochrome P450 3A4 Drug-Metabolizing Enzymes in Human Liver Microsomes
2
조회
0
다운로드
3
인용
0
참고문헌
키워드
초록
Objects : The aim of this study was to investigate the influence of five herbal medicines on cytochrome P450 3A4 drug-metabolizing enzymes in human liver microsomes. Methods : To use human liver microsomes, an extract of five herbal medicines, which are Artemisia princeps Pampan, Sophora japonica Linne, Panax notoginseng F. H. Chen, Lithospermum Erythrorhizon Sieb., and Cirsium maackii Maxim, which together are called Jihyulyak(止血藥, drugs for arresting bleeding, hemostatics), was co-incubated and measured for relative enzyme activity in incubation condition compared to ketoconazole, a representative inhibitor of CYP 3A4. Results : We showed that all five of the traditional herbal medicines had no inhibition effect of CYP 3A4 at 10, 20, 30, 40, and 50㎍/㎖ doses in human liver microsomes, although Sophora japonica Linne(SJL) showed a little inhibition at about 81% inhibition rate of control. However, this result is not enough to prove that SJL has a CYP 3A4 inhibition effect. Moreover, we can't make sure that those rates had significant induction effect on CYP 3A4. Conclusions : The result of this study could support that those herbal medicines are safer than chemical drugs, even if this is the basic step to prove that result.
Abstract
Objects : The aim of this study was to investigate the influence of five herbal medicines on cytochrome P450 3A4 drug-metabolizing enzymes in human liver microsomes. Methods : To use human liver microsomes, an extract of five herbal medicines, which are Artemisia princeps Pampan, Sophora japonica Linne, Panax notoginseng F. H. Chen, Lithospermum Erythrorhizon Sieb., and Cirsium maackii Maxim, which together are called Jihyulyak(止血藥, drugs for arresting bleeding, hemostatics), was co-incubated and measured for relative enzyme activity in incubation condition compared to ketoconazole, a representative inhibitor of CYP 3A4. Results : We showed that all five of the traditional herbal medicines had no inhibition effect of CYP 3A4 at 10, 20, 30, 40, and 50㎍/㎖ doses in human liver microsomes, although Sophora japonica Linne(SJL) showed a little inhibition at about 81% inhibition rate of control. However, this result is not enough to prove that SJL has a CYP 3A4 inhibition effect. Moreover, we can't make sure that those rates had significant induction effect on CYP 3A4. Conclusions : The result of this study could support that those herbal medicines are safer than chemical drugs, even if this is the basic step to prove that result.
참고문헌 (32)
- Guengerich FP, "Cytochrome P450s and other enzymes in drug metabolism and toxicity", AAPS J, 8(1), pp.E101~E111 (2006)
- Guengerich FP, "Cytochromes P450, drugs, and diseases", Mol Interv, 3(4), pp.194~204 (2003)
- Guengerich FP, "Characterization of human cytochrome P450 enzymes", FASEB J, 6(2), pp.745~748 (1992)
- Iwata, H., "Inhibition of human liver microsomal CYP3A4 and CYP2D6 by extracts from 78 herbal medicines", Journal of Traditional Medicines, 21(1), pp.42~50 (2004)
- Takahashi K, "In vitro inhibitory effects of Kampo medicines on metabolic reactions catalyzed by human liver microsomes", J Clin Pharm Ther, 28(4), pp.319~327 (2003)
- Makino T, "Does a kampo medicine containing schisandra fruit affect pharmacokinetics of nifedipine like grapefruit juice?", Biol Pharm Bull, 29(10), pp.2065~2069 (2006)
- 서울대학교 의과대학, "임상약리학", pp.211~ (2006), 서울대학교출판부
- Pal D, "MDR- and CYP3A4 -mediated drug-herbal interactions", Life Sci, 78(18), pp.2131~2145 (2006)
- Shufeng Zhou, "Interactions of Herbs with Cytochrome P450", Drug Metabolism, 35(1), pp.35~98 (2003)
- Lee SK, "Characterization of in vitro metabolites of deoxypodophyllotoxin in human and rat liver microsomes using liquid chromatography", tandem mass spectrometry, 2008, pp.22(1), 52~58
- 許浚, "東醫寶鑑", pp.418~ (2005), 法仁文化社
- 全國韓醫科大學本草學敎室, "本草學", pp.196~ (2000), 永林社
- Park JY, "Effect of ketoconazole on the pharmacokinetics of rosiglitazone in healthy subjects", Br J Clin Pharmacol, 58(4), pp.397~402 (2004)
- Davidson MH, "Does differing metabolism by cytochrome p450 have clinical importance?", Curr Atheroscler Rep, 2(1), pp.14~19 (2000)
- Emoto C, "Relative roles of CYP2C19 and CYP3A4", 5 in midazolam Xenobiotica, 2007, pp.37(6), 592~603
- 서울대학교 약제부, "Pharmacy Newsletter of Drug Information", 24(9) (2004)
- Flockhart DA, "Selection of drugs to treat gastro-oesophageal reflux disease: the role of drug interactions", Clin Pharmacokinet, 39(4), pp.295~309 (2000)
- Martignoni M, "Species differences between mouse, rat, dog, monkey and human CYP-mediated drug metabolism, inhibition and induction", Expert Opin Drug Metab Toxicol, 2(6), pp.875~894 (2006)
- Jan WC, "Tsai TH. Herb-drug interaction of Evodia rutaecarpa extract on the pharmacokinetics of theophylline in rats", J Ethnopharmacol, 102(3), pp.440~445 (2005)
- Johnston BC, "Herb-drug interaction implications in pediatric practice: an overview of pharmacokinetic trials", Arch Pediatr Adolesc Med, 159(7), pp.692~ (2005)
- Braun L., "Herb-drug interaction guide", Fam Physician, 30(2), pp.151~ (2001)
- 김봄님, "艾葉 및 阿膠藥鍼이 止血效果에 미치는 影響", 대한한방부인과학회지, 13(2), pp.46~59 (2000)
- 최윤영, "애엽(艾葉) 추출(抽出) 약침(藥鍼)이 허혈성(虛血性) 손상(損傷)에 미치는 효과(效果)", 경락경혈학회지, 21(1), pp.15~27 (2004)
- 김재효, "흰쥐의 一過性 前腦 虛血 損傷에 대한 艾葉 藥鍼의 신경보호 작용", 대한한의학회지, 24(2), pp.81~93 (2003), 대한한의학회
- 윤성묵, "애엽(艾葉) 약침액(藥鍼液)에 의한 Phase II 효소 활성 유도", 경락경혈학회지, 18(1), pp.1~9 (2001)
- 박성진, "槐花의 식품영양학적 접근 및 생리활성물질 함량분석", 동의생리병리학회지, 21(1), pp.171~180 (2007), 대한동의병리학회
- 이지은, "괴화 추출물이 대식세포에서의 nitric oxide와 interleukin-6의 생성에 미치는 영향", 대한치주과학회지, 35(1), pp.9~20 (2005), 대한치주과학회
- 박정애, "三七根이 瘀血病態模型에 미치는 影響", 동의병리학회지, 7, pp.15~26 (1992)
- 박용기, "三七根의 LPS에 의해 활성화된 腦神經膠細胞로부터의 炎症媒介物質 生成抑制效果", 대한본초학회지, 21(4), pp.93~101 (2006), 대한본초학회
- 김시혜, "紫草가 아토피 피부염에 미치는 영향", 대한한방소아과학회지, 18(1), pp.63~75 (2004)
- 권미화, "紫草가 항알러지 염증반응에 미치는 영향", 대한한의학회지, 26(3), pp.110~123 (2005), 대한한의학회
- 김혁, "大？ 추출물의 활성산소 소거능 및 Cytochrome P450 효소 저해효과", 대한본초학회지, 22(1), pp.53~62 (2007), 대한본초학회