열다한소탕과 청심연자탕의 Cytochrome P450 활성 연구
Effects of Yuldahanso-tang and Chungsimyonja-tang on Cytochrome P450 Activities
2
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Objectives The purpose of this study is to investigate the inhibitory or inductive potentials of Yuldahanso-tang (YDT) and Chungsimyonja-tang (CST), herbal formulas for Taeeumin, on cytochrome P450 (CYP450) drug metabolizing enzyme. The mechanisms for the herbal formula-drug interaction has not been well reported in spite of the chance for co-administration with conventional drugs. Methods To evaluate the interaction potential of YDT-drug or CST-drug, the fluorescence-based enzyme assays on CYP450 isozymes including CYP3A4, CYP2C19, CYP2D6 and CYP2E1 were established in vitro. The inhibitory effects of herbal formulas were characterized with IC50 values. Results YDT showed inhibitory effects on CYP2D6 and CYP2E1-mediated metabolism, while it exhibited week inhibition on CYP3A4 and CYP2C19 relatively. CST exerted relatively weak inhibitory effects on the four CYP450 isozymes compared to that of YDT. Conclusions These results suggest that the herbal formula-drug interaction could be occur when YDT are co-administered with drugs mediated by CYP2D6 or CYP2E1.
Abstract
Objectives The purpose of this study is to investigate the inhibitory or inductive potentials of Yuldahanso-tang (YDT) and Chungsimyonja-tang (CST), herbal formulas for Taeeumin, on cytochrome P450 (CYP450) drug metabolizing enzyme. The mechanisms for the herbal formula-drug interaction has not been well reported in spite of the chance for co-administration with conventional drugs. Methods To evaluate the interaction potential of YDT-drug or CST-drug, the fluorescence-based enzyme assays on CYP450 isozymes including CYP3A4, CYP2C19, CYP2D6 and CYP2E1 were established in vitro. The inhibitory effects of herbal formulas were characterized with IC50 values. Results YDT showed inhibitory effects on CYP2D6 and CYP2E1-mediated metabolism, while it exhibited week inhibition on CYP3A4 and CYP2C19 relatively. CST exerted relatively weak inhibitory effects on the four CYP450 isozymes compared to that of YDT. Conclusions These results suggest that the herbal formula-drug interaction could be occur when YDT are co-administered with drugs mediated by CYP2D6 or CYP2E1.
참고문헌 (24)
- Parola M, "Oxidative stress-related molecules and liver fibrosis", J Hepatol, 35, pp.297~306 (2001)
- 박영철, "독성학적 측면에서의 한약에 의한 간독성 유발과 기전", 대한한의학회지, 32(4), pp.48~67 (2011), 대한한의학회
- Henderson GL, "Effects of ginseng components on c-DNA-expressed cytochrome P450 enzyme catalytic activity", Life Sci, 65, pp.PL209~PL214 (1999)
- Paolini M, "Effect of licorice and glycyrrhizin on murine liver CYP-dependent monooxygenases", Life Sci, 62, pp.571~582 (1998)
- Tang JC, "Effect of the water extract and ethanol extract from traditional Chinese medicines Angelica sinensis (Oliv.) Diels, Ligusticum chuanxiong Hort. and Rheum palmatum L. on rat liver cytochrome P450 activity", Phytother Res, 20, pp.1046~1051 (2006)
- Guerraa MC, "Accelerated communication Comparison between Chinese medical herb Pueraria lobata crude extract and its main isoflavone puerarin : Antioxidant properties and effects on rat liver CYP-catalysed drug metabolism", Life Sciences, 67, pp.2997~3006 (2000)
- 정혜광, "생약추출물의 Cytochrome P450 약물대사 효소계 저해활성", 생약학회지, 33(1), pp.35~41 (2002), 한국생약학회
- Liu Y, "Early metabolism evaluation making traditional Chinese medicine effective and safe therapeutics", J Zhejiang Univ Sci B, 7(2), pp.99~106 (2006)
- Zhou S, "Interactions of herbs with cytochrome P450", Drug Metab Rev, 35(1), pp.35~98 (2003)
- Kim KY, "Consideration about medicine historical view of Je-Ma Lee", J Sasang Constitut Med, 6(1), pp.11~19 (1994)
- National federation of department of sasang constitutional medicine, colleges of oriental medicine, "Sasang constitutional medicine. 2nd Ed", pp.418~420 (2004), Jipmoondang
- Choi YJ, "Effects of yuldahansotang water extract on blood pressure, regional cerebral blood flow and pial arterial diamter", J Sasang Constitut Med, 10(1), pp.285~293 (1998)
- Choi JS, "Studies on the cytokine production regulation in human astrocytes by Yuldahansotang", J Sasang Constitut Med, 13(1), pp.61~69 (2001)
- Lee JH, "Effects of Yuldahansotang water extract on cultured primary hippocampal cell culture damaged by hydrogen peroxide", J Sasang Constitut Med, 14(1), pp.79~89 (2002)
- Hong JH, "Effects of Yuldahansotang water extract on cultured spinal sensory neurons damaged by xanthine oxidase", hypoxanthine, 2001, pp.13(1), 88~96
- 임종필, "太陰人 淸心蓮子湯이 SD rat 의 抗酸化 및 免疫調節效果에 대한 실험적 연구", 사상체질의학회지, 19(3), pp.227~241 (2007), 사상체질의학회
- Park SC, "Effects ChungSimYeonJaTang (CSYJT) on control of immune-function in highly purified mouse B cell and Mast cell", J Sasang Constitut Med, 15(2), pp.166~179 (2003)
- Hong SC, "An experimental study on the anti-stress effect by taeeumin Chongsimyonjatang", J Sasang Constitut Med, 7(2), pp.227~240 (1995)
- Kato R, "Further studies on the inhibition and stimulation of microsomal drug-metabolizing enzymes of rat liver by various compounds", Biochem Pharmacol, 13, pp.69~83 (1964)
- Conney AH, "Pharmacological implication of microsomal enzyme induction", Pharmacol Rev, 19, pp.317~366 (1967)
- Neter KJ, "Inhibition of oxidative drug metabolism in microsomes", Pharm Ther, 10, pp.515~535 (1980)
- 박용기, "In vitro inhibitory effects of Wen-pi-tang-Hab-Wu-ling-san on human cytochrome P450 isoforms", JOURNAL OF CLINICAL PHARMACY AND THERAPEUTICS, 36(4), pp.496~503, WILEY-BLACKWELL
- 박영철, "한약의 약물상호작용 기전과 연구방향- 독성학적인 측면을 중심으로 -", 대한예방한의학회지, 15(1), pp.1~16 (2011), 대한예방한의학회
- Elizabeth LM, "Update : Clinically significant cytochrome P450 drug interactions", Pharmacotherapy, 18(1), pp.84~112 (1998)