Human Liver Microsome을 이용한 수종 이기약의간대사효소에 미치는 영향
The Effect to the Hepatic Metabolic Enzume that Uses Human Liver Microsome Caused by a Bariety of Yigiyak
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In this study, we experimented the influence of three herbal medicines, which are Saussurea lappa Clarke, Poncirus trifoliata Rafin, Citrus aurantium Linne, which are called ‘Yigiyak(理氣藥)’ on drug metabolizing enzyme cytochrome P450 3A4 in Human Liver Microsome. Above all, the reason for this study is that herbal medicines can be assumed that herbs might have interactions with drugs, other herbs, alcohol and chemicals whether those are much better synergy effects than expected effects when the medicine was treated alone or not. As a result, we showed that all of five traditional herbal medicines had no CYP 3A4 inhibition effect on 10, 20, 30, 40, 50㎍/㎖ doses in Human Liver Microsome even Saussurea lappa Clarke showed a little inhibition as about 93% and 79% inhibition rate of control. However, this result are mostly not enough to prove that SLC has a CYP 3A4 inhibition effect. Moreover, it is not that those rates showed that those herbal medicines have CYP 3A4 induction effect. In conclusion, the result could support that those herbal medicines are more safe than chemical drugs even if this is the basic step to prove that result. Therefore, more specific studies to support this result, which are Kinetic study, cell and animal study then finally until clinical research, are required.
Abstract
In this study, we experimented the influence of three herbal medicines, which are Saussurea lappa Clarke, Poncirus trifoliata Rafin, Citrus aurantium Linne, which are called ‘Yigiyak(理氣藥)’ on drug metabolizing enzyme cytochrome P450 3A4 in Human Liver Microsome. Above all, the reason for this study is that herbal medicines can be assumed that herbs might have interactions with drugs, other herbs, alcohol and chemicals whether those are much better synergy effects than expected effects when the medicine was treated alone or not. As a result, we showed that all of five traditional herbal medicines had no CYP 3A4 inhibition effect on 10, 20, 30, 40, 50㎍/㎖ doses in Human Liver Microsome even Saussurea lappa Clarke showed a little inhibition as about 93% and 79% inhibition rate of control. However, this result are mostly not enough to prove that SLC has a CYP 3A4 inhibition effect. Moreover, it is not that those rates showed that those herbal medicines have CYP 3A4 induction effect. In conclusion, the result could support that those herbal medicines are more safe than chemical drugs even if this is the basic step to prove that result. Therefore, more specific studies to support this result, which are Kinetic study, cell and animal study then finally until clinical research, are required.
참고문헌 (19)
- 김창민, "中藥大辭典", pp.1388~3972 (1999), 정담
- 정운찬, "임상약리학", 서울대학교 의과대학편, ~ (2006)
- 許浚, "東醫寶鑑(全五卷中五)", pp.2969~3074 (1994), 여강출판사
- Emoto C, "Relative roles of CYP2C19 and CYP3A4", 5 inmidazolam 1'-hydroxylation, 2007, pp.37(6), 592~603
- Flockhart DA, "Selection of drugs to treat gastro-oesophageal reflux disease : the role of drug interactions Clin Pharmacokinet", 39(4), pp.295~309 (2000)
- Guengerich FP, "Characterization of human cytochrome P450 enzymes", FASEB J, 6(2), pp.745~748 (1992)
- Guengerich FP, "Cytochrome P450s and other enzymes in drug metabolism and toxicity", AAPS J, 8(1), pp.101~111 (2006)
- Guengerich FP, "Cytochrome P450 drugs and diseases", Mol Interv, 3(1), pp.194~204 (2003)
- Hellum BH, "The induction of CYP1A2, CYP2D6 and CYP3A4 by six trade herbal products in cultured primary human hepatocytes", Basic Clin Pharmacol Toxicol, 100(1), pp.23~30 (2007)
- Iwata H, "Inhibition of human liver microsomal CYP3A4 and CYP2D6 by extracts from 78 herbal medicines", Journal of Traditional Medicines, 21(1), pp.42~50 (2004)
- Jiang X, "Investigation of the effects of herbal medicines on warfarin response in healthy subjects : apopulationpharmacokinetic-pharmacodynamic modeling approach", Clin Pharmacol, 46(11), pp.1370~1378 (2006)
- Lee SK, "Characterization of in vitro metabolites of deoxypodophyllotoxin in human and rat liver microsomes using liquid chromatography", tandem mass spectrometry, 2008, pp.22(1), 58~58
- Makino T, "Does a kampo medicine containing schisandra fruit affect pharmacokinetics of nifedipine like grapefruit juice", Biol Pharm Bull, 29(10), pp.2065~2069 (2006)
- Martignoni M, "Species differences between mouse rat dog monkey and human CYP-mediated drug metabolism, inhibition and induction", Expert Opin Drug Metab Toxicol, 2, pp.875~894 (2006)
- Pal D, "MDR- and CYP3A4- 이현호 외 2인：Human Liver Microsome을 이용한 수종 이기약의 간대사효소에 미치는 영향 49 mediated drug-herbal interactions", Life Sci, 27(18), pp.2131~2145 (2006)
- 서울대학교 약제부, "Pharmacy Newsletter of Drug Information", 24(9) (2004)
- Takahashi K, "Invitro inhibitory effects of Kampo medicines on metabolic reactions catalyzed by human liver microsomes", Clin Pharm Ther, 28(4), pp.319~327 (2003)
- Shufeng Zhou, "Interactions of Herbs with Cytochrome", Drug Metabolism, 450~ (2003)
- Yao HT, "The inhibitory effect of tannic acid on cytochrome P450 enzymes and NADPHCYP reductase in rat and human liver microsomes", Food Chem Toxicol, 46(2), pp.645~653 (2008)