LC-MS/MS를 이용한 마비성패류독소 분석조건 검토
Paralytic Shellfish Poisoning (PSP) Analysis using Liquid Chromatography-Tandem Mass Spectrometry
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다운로드
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참고문헌
키워드
초록
The AOAC Mouse Bioassay method (MBA) has been widely used for routine monitoring of paralytic shellfish poisoning (PSP) for more than 50 years. However, this method has low sensitivity and experiences interference from other components in the extract. Also, ethical issues have been raised against the continued use of this live-mouse assay. To establish an alternative method for PSP analysis, we attempted to develop PSP analysis conditions using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS analysis of reference material showed very reasonable accuracy, and the analysis time was just 15 min. However, the recovery rate of toxin spike samples using the LC-MS/MS analysis was 59.4-91.0%. We also attempted to remove the matrix effect using shellfish extracts, but recoveries of C1 and C2 did not improve. A comparison between the results of MBA and LC-MS/MS analysis revealed good correlations, with values of 0.8878 and 0.9211 for oyster and mussel matrices, respectively.
Abstract
The AOAC Mouse Bioassay method (MBA) has been widely used for routine monitoring of paralytic shellfish poisoning (PSP) for more than 50 years. However, this method has low sensitivity and experiences interference from other components in the extract. Also, ethical issues have been raised against the continued use of this live-mouse assay. To establish an alternative method for PSP analysis, we attempted to develop PSP analysis conditions using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The LC-MS/MS analysis of reference material showed very reasonable accuracy, and the analysis time was just 15 min. However, the recovery rate of toxin spike samples using the LC-MS/MS analysis was 59.4-91.0%. We also attempted to remove the matrix effect using shellfish extracts, but recoveries of C1 and C2 did not improve. A comparison between the results of MBA and LC-MS/MS analysis revealed good correlations, with values of 0.8878 and 0.9211 for oyster and mussel matrices, respectively.
참고문헌 (18)
- Alpert AJ, "The term HILIC(Hydrophilic interaction liquid chromatograpy) was introduced by Alpert in 1990", J Chromatogra A, 499, pp.177~196 (1990)[DOI]
- Asp TN, "Analysis of PSP toxins in Norwegian mussels by a post-column derivatization HPLC method", Toxicon, 43, pp.319~327 (2004)[DOI]
- AOAC, "AOAC Official Method 959.08. Paralytic Shellfish Poison. Biological method. Final action, In AOAC Official methods for analysis, 18th Edition Chapter 49: Natural toxins", pp.79~80 (2005), AOAC International
- Ben-Gigirey B, "Influence of the sample toxic profile on the suitability of a high performance liquid chromatography method for official paralytic shellfish toxins control", J Chromatogra A, 1140, pp.78~87 (2007)[DOI]
- Bricelj VM, "Paralytic shellfish toxins in bivalve mollusks. Occurrence, transfer kinetics and biotransformation", Rev Fish Sci, 6, pp.315~383 (1998)
- Carmela DA, "Hydrophilic interaction liquid chromatography-mass spectrometry for the analysis of paralytic shellfish poisoning(PSP) toxins", J Chromatogra A, 1081, pp.190~201 (2005)[DOI]
- Report of the Thirtyfirst session, "Codex Alimentarius Commission", ~, pp.ALINORM 08 (2008), 31[DOI]
- Franco JM, "Separation of paralytic shellfish toxins by reversed phase high performance liquid chromatography with postcolumn reaction and fluorometric detection", Chromatographia, 35, pp.613~620 (1993)
- Hall S, "Marine Toxins: Origin, Structure, and Molecular Pharmacology", pp.29~65 (1990), American Chemical Society
- Hall S, "Natural toxins, In Microbiology of Marine Food Products", pp.301~330 (1991), Van Nostrand Reinhold
- Lawrence JF, "Liquid chromatographic determination of paralytic shellfish poisons in shellfish after prechromatographic oxidation", J Assoc Off Anal Chem, 74, pp.1006~1012 (1991)
- Lawrence JF, "Quantitative determination of paralytic shellfish poisoning toxins in shellfish using prechromatographic oxidation and liquid chromatography with fluorescence detection: collaborative study", J AOAC Int, 88, pp.1714~1732 (2005)
- Luckas B, "Analytical methods for paralytic shellfish poisons, In Manual on Harmful Marine Microalgae", pp.191~209 (2003), UNESCO
- Mortensen AM, "Massive fish mortalities in the Faroe Islands caused by a Gonyaulax excavate red tide, In Toxic Dinoflagellates", pp.165~170 (1985), Elsevier
- Oshima Y, "Post-column derivatization liquid-chromatograpic method for paralytic shellfish toxins", J AOAC Int, 78, pp.528~532 (1995)
- Quilliam MA, "Ionspray mass spectrometry of marine neurotoxins", Rapid Commun Mass Spectrom, 3, pp.145~150 (1989)
- Thomas K, "Analysis of PSP toxins by liquid chromatography with post column oxidation and fluorescence detection, In Molluscan Shellfish Safety", pp.132~138 (2006), The Marine Institute
- Van de Riet JM, "Liquid chromatographic post-column oxidation method for analysis of paralytic shellfish toxins in mussels, clams, scallops, and oysters: single-laboratory validation", J AOAC Int, 92, pp.1690~1704 (2009)