마비성패류독소 검출을 위한 분석법 비교
Comparison of Analytical Methods for the Detection of Paralytic Shellfish Toxins (PSTs)
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초록
Paralytic shellfish toxins (PSTs) are produced by marine dinoflagellate phytoplankton Alexandrium spp. and Gymnodiniumspp. These toxins accumulate in filter feeding organisms such as bivalves and the ingestion of contaminatedshellfish can cause illness in humans. The mouse bioassay (MBA) has been the preferred PST testing methodworldwide for more than 50 years. However, this assay has several disadvantages, such as detection limits, non-toxicprofiles,and the ethical issues of using animals. The aim of this study was to establish an alternative to the MBAmethod for testing for PSTs. We optimized the analysis conditions of a post-column oxidation-high performanceliquid chromatography (PCOX-HPLC) method and the Scotia Rapid Test Kit, and then compared the accuracy ofthese methods to the MBA method. The results demonstrated a strong correlation between the PCOX-HPLC methodand the MBA, although the PCOX-HPLC method required expensive equipment and standard material, and wastime consuming. The Scotia Rapid Test Kit promises to be a useful tool, as it provided rapid and qualitative results,although the method sometimes gave a false positive result that could not be explained by toxin profiles.
Abstract
Paralytic shellfish toxins (PSTs) are produced by marine dinoflagellate phytoplankton Alexandrium spp. and Gymnodiniumspp. These toxins accumulate in filter feeding organisms such as bivalves and the ingestion of contaminatedshellfish can cause illness in humans. The mouse bioassay (MBA) has been the preferred PST testing methodworldwide for more than 50 years. However, this assay has several disadvantages, such as detection limits, non-toxicprofiles,and the ethical issues of using animals. The aim of this study was to establish an alternative to the MBAmethod for testing for PSTs. We optimized the analysis conditions of a post-column oxidation-high performanceliquid chromatography (PCOX-HPLC) method and the Scotia Rapid Test Kit, and then compared the accuracy ofthese methods to the MBA method. The results demonstrated a strong correlation between the PCOX-HPLC methodand the MBA, although the PCOX-HPLC method required expensive equipment and standard material, and wastime consuming. The Scotia Rapid Test Kit promises to be a useful tool, as it provided rapid and qualitative results,although the method sometimes gave a false positive result that could not be explained by toxin profiles.
참고문헌 (19)
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