HPLC에 의한 Domoic acid 분석법 검정
Validation of the Analysis of Domoic Acid using High Performance Liquid Chromatography
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To prevent amnesic shellfish poisoning (ASP) resulting from the consumption of shellfish contaminated with domoic acid, the quantitative analysis of domoic acid is very important. We validated a high performance liquid chromatography (HPLC) method for accurate and precise quantification of domoic acid. A clear peak and the isolation of domoic acid resulted on injecting a 50% methanol extract of CRM-ASP-Mus-c mussel reference material using HPLC. The limit of detection of domoic acid under the established HPLC conditions was 0.10 μg/g, and the limit of quantification of the toxin under the same conditions was 0.25 μg/g. The intra-accuracy and precision for domoic acid in CRM-ASP-Mus-c were 90.7–95.7% and 0.28–2.25%, respectively. The inter-accuracy and precision for domoic acid were 89.1–97.1% and 1.7–4.1%, respectively. The mean recovery of domoic acid in methanol extracts from ten species of marine invertebrates was 88.6–105.1%.
Abstract
To prevent amnesic shellfish poisoning (ASP) resulting from the consumption of shellfish contaminated with domoic acid, the quantitative analysis of domoic acid is very important. We validated a high performance liquid chromatography (HPLC) method for accurate and precise quantification of domoic acid. A clear peak and the isolation of domoic acid resulted on injecting a 50% methanol extract of CRM-ASP-Mus-c mussel reference material using HPLC. The limit of detection of domoic acid under the established HPLC conditions was 0.10 μg/g, and the limit of quantification of the toxin under the same conditions was 0.25 μg/g. The intra-accuracy and precision for domoic acid in CRM-ASP-Mus-c were 90.7–95.7% and 0.28–2.25%, respectively. The inter-accuracy and precision for domoic acid were 89.1–97.1% and 1.7–4.1%, respectively. The mean recovery of domoic acid in methanol extracts from ten species of marine invertebrates was 88.6–105.1%.
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