뱀장어(Anguilla japonica) 추출 Carnosine이 과산화수소로 유도된 인체 백혈구의 DNA 손상과 Repair에 미치는 효과
The Effect of Carnosine Extracted from Eels Anguilla japonica on Oxidative DNA Damage Induced by H2O2 and the DNA Repair Capacity of Human Leukocytes
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초록
Carnosine was recently reported to protect against the DNA damage induced by oxidative stress. In this study, we investigated the protective effect of eel Anguilla japonica carnosine extracts prepared using different methods (heat treatment extracts, HTEs; ion exchange chromatography, IEC; ultrafiltration permeation, UFP) on leukocyte DNA damage using the comet assay. Human leukocytes were incubated with extracts of eel carnosine at concentrations (of 10, 50, 100 μg/mL), and then subjected to an oxidative stimulus [200 μM hydrogen peroxide (H2O2)]. Pretreatment of the cells for 30 min with carnosine significantly reduced the genotoxicity of H2O2 measured as DNA strand breaks. The protective effects of the three types of extract (HTE, IEC, and UFP) increased with concentration. At the highest concentration (100 g/mL). there were no statistical differences in oxidative damage between each extract treatment and PBS-treated negative controls. When leukocytes were incubated with carnosine for 30 min after exposure to H2O2. the protective ability of each extract changed. Therefore, eel carnosine inhibits the H2O2 induced damage to cellular DNA in human leukocytes, supporting the protective effect of this compound against oxidative damage.
Abstract
Carnosine was recently reported to protect against the DNA damage induced by oxidative stress. In this study, we investigated the protective effect of eel Anguilla japonica carnosine extracts prepared using different methods (heat treatment extracts, HTEs; ion exchange chromatography, IEC; ultrafiltration permeation, UFP) on leukocyte DNA damage using the comet assay. Human leukocytes were incubated with extracts of eel carnosine at concentrations (of 10, 50, 100 μg/mL), and then subjected to an oxidative stimulus [200 μM hydrogen peroxide (H2O2)]. Pretreatment of the cells for 30 min with carnosine significantly reduced the genotoxicity of H2O2 measured as DNA strand breaks. The protective effects of the three types of extract (HTE, IEC, and UFP) increased with concentration. At the highest concentration (100 g/mL). there were no statistical differences in oxidative damage between each extract treatment and PBS-treated negative controls. When leukocytes were incubated with carnosine for 30 min after exposure to H2O2. the protective ability of each extract changed. Therefore, eel carnosine inhibits the H2O2 induced damage to cellular DNA in human leukocytes, supporting the protective effect of this compound against oxidative damage.
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