Protective effects of carnosine and homocarnosine on ferritin and hydrogen peroxide-mediated DNA damage
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Previous studies have shown that one of the primary causes of increased iron content in the brain may be the release of excess iron from intracellular iron storage molecules such as ferritin. Free iron generates ROS that cause oxidative cell damage. Carnosine and related compounds such as endogenous histidine dipetides have antioxidant activities. We have investigated the protective effects of carnosine and homocarnosine against oxidative damage of DNA induced by reaction of ferritin with H2O2. The results show that carnosine and homocarnosine prevented ferritin/H2O2-mediated DNA strand breakage. These compounds effectively inhibited ferritin/H2O2-mediated hydroxyl radical generation and decreased the mutagenicity of DNA induced by the ferritin/H2O2 reaction. Our results suggest that carnosine and related compounds might have antioxidant effects on DNA under pathophysiological conditions leading to degenerative damage such as neurodegenerative disorders.
Abstract
Previous studies have shown that one of the primary causes of increased iron content in the brain may be the release of excess iron from intracellular iron storage molecules such as ferritin. Free iron generates ROS that cause oxidative cell damage. Carnosine and related compounds such as endogenous histidine dipetides have antioxidant activities. We have investigated the protective effects of carnosine and homocarnosine against oxidative damage of DNA induced by reaction of ferritin with H2O2. The results show that carnosine and homocarnosine prevented ferritin/H2O2-mediated DNA strand breakage. These compounds effectively inhibited ferritin/H2O2-mediated hydroxyl radical generation and decreased the mutagenicity of DNA induced by the ferritin/H2O2 reaction. Our results suggest that carnosine and related compounds might have antioxidant effects on DNA under pathophysiological conditions leading to degenerative damage such as neurodegenerative disorders.
참고문헌 (33)
- Knovich, M. A., "Ferritin for the clinician", Blood Rev., 23, pp.95~104 (2009)
- Monterio, H., "Release of iron from ferritin by semiquinone, anthracycline, bipyridyl, and nitroaromatic radicals", Free Radic. Biol. Med., 6, pp.587~591 (1989)
- Lapenna, D., "Cigarette smoke, ferritin, and lipid peroxidation", Am. J. Respir. Crit. Care Med., 151, pp.431~435 (1995)
- Linert, W., "Dopamine, 6-hydroxydopamine, iron, and dioxygen-their mutual interactions and possible implication in the development of Parkinson's disease", Biochim. Biophys. Acta., 1316, pp.160~168 (1996)
- Double, K. L., "In vitro studies of ferritin iron release and neurotoxicity", J. Neurochem., 70, pp.2492~2499 (1998)
- Monterio, H., "The superoxide-dependent transfer of iron from ferritin to transferrin and lactoferrin", Biochem. J., 256, pp.923~928 (1988)
- Boyer, R., "Reductive release of ferritin iron: a kinetic assay", Anal. Biochem., 174, pp.17~22 (1988)
- Halliwell, B., "The importance of free radicals and catalytic metal ions in human diseases", Mol. Aspects Med., 8, pp.89~193 (1985)
- Gotz, E., "Oxidative stress: free radical production in neural degeneration", Pharmacol. Ther., 63, pp.37~122 (1994)
- Hyslop, P. A., "Measurement of striatal H2O2 by microdialysis following global forebrain ischemia and reperfusion in the rat: correlation with the cytotoxic potential of H2O2 in vitro", Brain Res., 671, pp.181~186 (1995)
- Gius, D., "Redox signaling in cancer biology", Antioxid. Redox. Signal., 8, pp.1249~1252 (2006)
- Behl, C., "Hydrogen peroxide mediates amyloid beta protein toxicity", Cell, 77, pp.817~827 (1994)
- Hipkiss, A. R., "Carnosine, a protective, anti-ageing peptide", Int. J. Biochem. Cell Biol., 30, pp.863~868 (1998)
- Alhamdani, M. S., "Antiglycation and antioxidant effect of carnosine against glucose degradation products in peritoneal mesothelial cells", Nephron. Clin. Pract., 107, pp.c26~c34 (2007)
- Decker, E. A., "A re-evaluation of the antioxidant activity of purified carnosine", Biochemistry (Mosc), 65, pp.766~770 (2000)
- Boldyrev, A., "Protection of neuronal cells aginst reactive oxygen species by carnosine and related compounds", Comp. Biochem. Physiol., 137, pp.81~88 (2004)
- Fu, Q., "Carnosine protects against Abeta41-induced neurotoxicity in differentiated rat PC12 cells", Cell Mol. Neurobiol., 28, pp.307~316 (2008)
- Lowery Jr. T. J., "Kinetic studies of iron deposition in horse spleen ferritin using H2O2 and O2 as oxidants", Biophys. Chem., 111, pp.173~181 (2004)
- Zastawny, T. H., "DNA base modifications and membrane damage in cultured mammalian cells treated with iron ions", Free Radic. Biol. Med., 18, pp.1013~1022 (1995)
- Helbock, H. J., "8-Hydroxydeoxyguanosine and 8-hydroxyguanine as biomarkers of oxidative DNA damage", Methods Enzymol., 300, pp.156~166 (1999)
- Boldyrev, A. A., "Antioxidative properties of histidine-containing dipeptides from skeletal muscles of vertebrates", Comp. Biochem. Physiol., 89, pp.245~250 (1988)
- Auroma, O. I., "Carnosine, homocarnosine and anserine: could they act as antioxidants in vivo?", Biochem. J., 264, pp.863~869 (1989)
- Brown, C. E., "Interactions among carnosine, anserine, ophidine and copper in biochemical adaptation", J. Theor. Biol., 88, pp.245~256 (1981)
- Decker, E. A., "Differences in the Antioxidant mechanism of carnosine in the presence of copper and iron", J. Agric. Food Chem., 40, pp.756~759 (1992)
- Aldini, G., "Carnosine is a quencher of 4-hydroxy-nonenal: through what mechanism of reaction", Biochem. Biophys. Res. Commun., 298, pp.699~706 (2002)
- Halliwell, B., "Formation of thiobarbituric-acid-reactive substance from deoxyribose in the presence of iron salts: the role of superoxide and hydroxyl radicals", FEBS Lett., 128, pp.347~352 (1981)
- Boveries, A., "The cellular production of hydrogen peroxide", Biochem. J., 128, pp.617~630 (1972)
- Imlay, J. A., "Assay of metabolic superoxide production in Escherichia coli", J. Biol. Chem., 266, pp.6957~6965 (1991)
- Turrens, J. F., "Mitochondrial generation of oxygen radicals during reoxygenation of ischemic tissues", Free. Radic. Res. Comms., 12, pp.681~689 (1991)
- D’Agostino, D. P., "Acute hyperoxia increases lipid peroxidation and induces plasma membrane blebbing in human U87 glioblastoma cells", Neuroscience, 159, pp.1011~1022 (2009)
- McBride, T. J., "Mutagenic spectrum resulting from DNA damage by oxygen radicals", Biochemistry, 30, pp.207~213 (1991)
- Kohen, R., "Antioxidant activity of carnosine, homocarnosine, and anserine present in muscle and brain", Proc. Natl. Acad. Sci. U.S.A., 통권 : 85, pp.3175~3179 (1988)
- Chan, W. K. M., "EPR-spin trapping studies of the hydroxyl radical scavenging activity of carnosine and related dipeptides", J. Agric. Food Chem., 42, pp.1407~1410 (1994)