Characterization of antioxidative peptide purified from black eelpout (Lycodes diapterus) hydrolysate
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The functional peptides from protein hydrolysates of various fishery sources have been identified such as antioxidant activity. The main intention of this study was purification and characterization of antioxidative peptide from black eelpout muscle. The antioxidative peptides were purified from black eelpout (Lycodes diapterus) muscle using different proteases. Antioxidant activity of black eelpout hydrolysates was evaluated using DPPH radical scavenging activity. Among six hydrolysates, the pepsin hydrolysate had the highest antioxidant activity compared to the other hydrolysates. Therefore, it was further purified and a peptide with seven amino acid residues of DLVKVEA (784 Da) was identified by amino acid sequence analysis. The EC50 value for scavenging DPPH radicals by purified peptide was 688.77 μM. Additionally, the purified peptide exhibited protective effect against DNA damage induces by oxidation in mouse macrophages (RAW 264.7 cells). The results of this study suggest that black eelpout muscle protein hydrolysate could potentially contribute to development of bioactive peptides in basic research.
Abstract
The functional peptides from protein hydrolysates of various fishery sources have been identified such as antioxidant activity. The main intention of this study was purification and characterization of antioxidative peptide from black eelpout muscle. The antioxidative peptides were purified from black eelpout (Lycodes diapterus) muscle using different proteases. Antioxidant activity of black eelpout hydrolysates was evaluated using DPPH radical scavenging activity. Among six hydrolysates, the pepsin hydrolysate had the highest antioxidant activity compared to the other hydrolysates. Therefore, it was further purified and a peptide with seven amino acid residues of DLVKVEA (784 Da) was identified by amino acid sequence analysis. The EC50 value for scavenging DPPH radicals by purified peptide was 688.77 μM. Additionally, the purified peptide exhibited protective effect against DNA damage induces by oxidation in mouse macrophages (RAW 264.7 cells). The results of this study suggest that black eelpout muscle protein hydrolysate could potentially contribute to development of bioactive peptides in basic research.
참고문헌 (41)
- Racheal Abuine, "Biological activity of peptides purified from fish skin hydrolysates", Fisheries and Aquatic Sciences, 22(2), pp.1~14 (2019), 한국수산과학회[DOI]
- Official methods of analysis, "Association of Official Analytical Chemist (AOAC)", Association of Official Analytical Chemist, ~ (2000)
- Becker GL, "Preserving food and health : antioxidants make functional, nutritious preservatives", Food Process, 12, pp.54~56 (1993)
- Bougatef A, "Purification and identification of novel antioxidant peptides from enzymatic hydrolysates of sardinelle(Sardinella aurita)by-products proteins", Food Chem, 118, pp.559~565 (2010)
- Byun HG, "Antioxidant peptides isolated from the marine rotifer, Brachionus rotundiformis", Process Biochem, 44, pp.842~846 (2009)
- Castro L, "Reactive oxygen species in human health and disease", Nutrition, 170, pp.161~165 (2001)
- Clemente A, "Enzymatic protein hydrolysates in human nutrition", Trends Food Sci Technol, 11, pp.254~262 (2000)
- Dávalos A, "Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis", J Food Prot, 67, pp.1939~1944 (2004)
- Dong S, "Antioxidant and biochemical properties of protein hydrolysates prepared from silver carp(Hypophthalmichthys molitrix)", Food Chem, 107, pp.1485~1493 (2008)
- Elias RJ, "Antioxidant activity of proteins and peptides", Crit Rev Food Sci Nutr, 48, pp.430~441 (2008)
- Gomez-Guillen MC, "Structural and physical properties of gelatin extracted from different marine species : a comparative study", Food Hydrocoll, 16, pp.25~34 (2002)
- Himaya SWA, "An active peptide purified from gastrointestinal enzyme hydrolysate of Pacific cod skin gelatin attenuates angiotensin-1 converting enzyme(ACE)activity and cellular oxidative stress", Food Chem, 132, pp.1872~1882 (2012)
- R. Jai ganesh, "Purification and Identification of Antioxidant Peptide from Black Pomfret, Parastromateus niger (Bloch, 1975) Viscera Protein Hydrolysate", Food Science and Biotechnology, 20(4), pp.1087~1094 (2011), 한국식품과학회
- Je JY, "Antioxidant and antihypertensive protein hydrolysates produced from tuna liver by enzymatic hydrolysis", Food Res Int, 42, pp.1266~1272 (2009)
- Je JY, "Purification and characterization of an antioxidant peptide obtained from tuna backbone protein by enzymatic hydrolysis", Process Biochem, 42, pp.840~846 (2007)
- Kim SK, "Isolation and characterization of antioxidative peptides from gelatin hydrolysate of Alaska Pollack skin", J Agric Food Chem, 49, pp.1984~1989 (2001)
- Korhonen M, "Impact of processing on bioactive proteins and peptides", Trends Food Sci Technol, 9, pp.307~319 (1998)
- Lee WS, "Characterization of a novel antioxidative peptide from the sand eel Hypoptychus dybowskii", Process Biochem, 46, pp.1207~1211 (2011)
- Woo-Shin Lee, "Antioxidant Activities of Steamed Extract from Squid(Todarodes pacificus) Muscle", Preventive Nutrition and Food Science, 16(2), pp.127~134 (2011), 한국식품영양과학회
- Li B, "Isolation and identification of antioxidative peptides from porcine collagen hydrolysate by consecutive chromatography and electrospray ionization-mass spectrometry", Food Chem, 102(4), pp.1135~1143 (2007)
- Liceaga-Gesualdo AM, "Functional properties of fish protein hydrolysate from herring(Clupea harengus)", J Food Sci, 64, pp.1000~1004 (1999)
- Martinez GR, "Oxidative and alkylating damage in DNA", Mutat Res, 554, pp.115~127 (2003)
- McCord JM, "Human disease. Free radicals, and the oxidant", antioxidant balance, 1993, pp.26, 351~357
- Murase H, "Antioxidant and emulsifying activity of N-(longchain-acyl)histidine and N-(long-chain-acyl)carnosine", J Agric Food Chem, 41, pp.1601~1604 (1993)
- Nazeer RA, "In vitro and in vivo studies on the antioxidant activity of fish peptide isolated from the croaker(Otolithes ruber)muscle protein hydrolysate", Peptides, 35, pp.261~268 (2012)
- Ngo DH, "In vitro antioxidant activity of a peptide isolated from Nile tilapia(Oreochromis niloticus)scale gelatin in free radical-mediated oxidative systems", J Funct Foods, 2, pp.107~117 (2010)
- Ngo DN, "Production of chitin oligosaccharides with different molecular weights and their antioxidant effect in RAW 264.7 cells", J Funct Foods, 1, pp.188~198 (2009)
- Park PJ, "Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin free egg yolk", J Am Chem Soc, 78, pp.651~656 (2001)
- Pihlanto L, "Bioactive peptides derived from bovine whey proteins : opioid and ace-inhibitory", Trends Food Sci Technol, 11, pp.347~356 (2000)
- Ren J, "Purification and identification of antioxidant peptides from grass carp muscle hydrolysates by consecutive chromatography and electrospray ionization-mass spectrometry", Food Chem, 108, pp.727~736 (2008)
- Samaranayaka AGP, "Food derived peptidic antioxidants : a review of their production, assessment, and potential applications", J Funct Foods, 3, pp.229~254 (2011)
- Sheih IC, "Antioxidant properties of a new antioxidative peptide from algae protein waste hydrolysate in different oxidation systems", Bioresour Technol, 100, pp.3419~3425 (2009)
- Somani SM, "Comparative effects of exercise training on transcription of antioxidant enzyme and the activity in old rat heart", Indian J Physiol Pharmacol, 40, pp.205~212 (1996)
- Tsuge N, "Antioxidative activity of peptides prepared by enzymatic hydrolsis of egg-white albumin", Nippon Nogeik Kaishi, 65, pp.1635~1641 (1991)
- Wanita A, "Antioxidant potential of 5-npentadecylresorcinol", J Food Process Pres, 20, pp.410~429 (1996)
- Wiriyaphan C, "Antioxidant activity of protein hydrolysates derived from threadfin bream surimi byproducts", Food Chem, 132, pp.104~111 (2012)
- Yen GC, "Antioxidative activity and scavenging effects on active oxygen of xylose-lysine Maillard reaction products", J Sci Food Agric, 67, pp.415~420 (1995)
- Yeung SY, "Scavenging property of three cresol isomers against H2O2, hypochlorite, superoxide and hydroxyl radicals", Food Chem Toxicol, 40, pp.1403~1413 (2002)
- You HJ, "Protective effect of metallothionein-III on DNA damage in response to reactive oxygen species", Biochim Biophys, 1573, pp.33~38 (2002)
- You L, "Purification and identification of antioxidative peptides from loach(Misgurnus anguillicaudatus)protein hydrolysate by consecutive chromatography and electrospray ionizationmass spectrometry", Food Res Int, 43, pp.1167~1173 (2010)
- Zhou DY, "Antioxidant activity of hydrolysates obtained from scallop(Patinopecten yessoensis)and abalone(Haliotis discus hannai Ino)muscle", Food Chem, 132, pp.815~822 (2012)
