다랑어(Thunnus) 추출물 중의 Histidine 함유 저분자 펩타이드 및 산화촉진물질 함량에 미치는 추출방법의 영향
Effects of Extraction Methods on Histidine-containing Low-molecular Weight Peptides and Pro-oxidants Contents in Tuna Thunnus Extracts
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초록
We investigated methods for extracting histidine-containing low-molecular-weight (LMW) peptides such as anserine,carnosine and histidine from the edible meat of tuna byproducts. Extracts were treated by several methods includingheat treatment (80℃, 10 min), DOWEX ion exchange (IEC), ultrafiltration (UF), and carboxymethyl (CM)-cellulosecolumn chromatography (IEC+CMC); then the levels of protein, total iron, histidine, carnosine, and anserine weremeasured. Extracts treated with IEC+CMC using CM-cellulose were analyzed for total iron, protein, histidine, andanserine content, which were 6.27±0.26 mg/mL, 5.20±0.21 μg/mL, 0.80 mg/mL, 0.208 mg/mL, and 4.40 mg/mL,respectively, in yellowfin tuna; and 9.05±0.82 mg/mL, 4.06±0.20 μg /mL, 1.62 mg/mL, 0.012 mg/mL, and 7.28 mg/mL in bigeye tuna. By comparison in IEC-UF treated extracts, protein, total iron, and histidine content decreased by43%, 73%, and 27% in yellowfin and 0.4%, 54%, and 23% in bigeye tuna, wheres carnosine and anserine content increasedby 22% and 17%, respectively. Freeze-dried (FD) extracts exhibited similar trends as non-dried extracts, i.e.,dipeptide content increased with purification steps, whereas pro-oxidant (total iron and protein) content decreased. IEC+CMC treated FD extracts had the highest anserine, content, and the greatest reductuion in pro-oxidants.
Abstract
We investigated methods for extracting histidine-containing low-molecular-weight (LMW) peptides such as anserine,carnosine and histidine from the edible meat of tuna byproducts. Extracts were treated by several methods includingheat treatment (80℃, 10 min), DOWEX ion exchange (IEC), ultrafiltration (UF), and carboxymethyl (CM)-cellulosecolumn chromatography (IEC+CMC); then the levels of protein, total iron, histidine, carnosine, and anserine weremeasured. Extracts treated with IEC+CMC using CM-cellulose were analyzed for total iron, protein, histidine, andanserine content, which were 6.27±0.26 mg/mL, 5.20±0.21 μg/mL, 0.80 mg/mL, 0.208 mg/mL, and 4.40 mg/mL,respectively, in yellowfin tuna; and 9.05±0.82 mg/mL, 4.06±0.20 μg /mL, 1.62 mg/mL, 0.012 mg/mL, and 7.28 mg/mL in bigeye tuna. By comparison in IEC-UF treated extracts, protein, total iron, and histidine content decreased by43%, 73%, and 27% in yellowfin and 0.4%, 54%, and 23% in bigeye tuna, wheres carnosine and anserine content increasedby 22% and 17%, respectively. Freeze-dried (FD) extracts exhibited similar trends as non-dried extracts, i.e.,dipeptide content increased with purification steps, whereas pro-oxidant (total iron and protein) content decreased. IEC+CMC treated FD extracts had the highest anserine, content, and the greatest reductuion in pro-oxidants.
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