Effect of Aeration-Agitation on Coenzyme Q10 Production Using Rhodobacter sphaeroides
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With the aim of increasing the CoQ10 production in mass culture, the effect of aeration-agitation on the CoQ10 production using Rhodobactor sphaeroides was investigated in a 1-L bioreactor. The maximum CoQ10 production was 1.69 mg/g of dry cell weight under conditions of 50 Lux, 30°C, 300 rpm, and 5-vvm aeration. The CoQ10 production was improved to produce 2.91 mg/g of dry cell weight under reduced conditions of agitation speed (200 rpm) and aeration rate (0.2 vvm). When R. sphaeroides was cultivated under more reduced DO levels during the exponential phase of the cell, the CoQ10 production yield of 3.88-mg/g dry cell weight was the maximum obtained. Therefore, poorer conditions of aeration-agitation resulted in higher production of CoQ10, and thus DO content was a crucial factor in the production of CoQ10. Accordingly, it was necessary to control the DO concentration in order to enhance the CoQ10 biosynthesis within a large-scale production.
Abstract
With the aim of increasing the CoQ10 production in mass culture, the effect of aeration-agitation on the CoQ10 production using Rhodobactor sphaeroides was investigated in a 1-L bioreactor. The maximum CoQ10 production was 1.69 mg/g of dry cell weight under conditions of 50 Lux, 30°C, 300 rpm, and 5-vvm aeration. The CoQ10 production was improved to produce 2.91 mg/g of dry cell weight under reduced conditions of agitation speed (200 rpm) and aeration rate (0.2 vvm). When R. sphaeroides was cultivated under more reduced DO levels during the exponential phase of the cell, the CoQ10 production yield of 3.88-mg/g dry cell weight was the maximum obtained. Therefore, poorer conditions of aeration-agitation resulted in higher production of CoQ10, and thus DO content was a crucial factor in the production of CoQ10. Accordingly, it was necessary to control the DO concentration in order to enhance the CoQ10 biosynthesis within a large-scale production.
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