본문 바로가기
KERD
논문KCI 등재

Optimization of Influencing Factors on Biomass Accumulation and 5-Aminolevulinic Acid (ALA) Yield in Rhodobacter sphaeroides Wastewater Treatment

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류생물학

초록

This study aimed to optimize four factors affecting biomass accumulation and 5-aminolevulinic acid (ALA) yield together with pollutants removal in Rhodobacter sphaeroides wastewater treatment. Results showed that it was feasible to produce biomass and ALA in R. sphaeroides wastewater treatment. Microaerobic, 1,000-3,000 lux, and pH 7.0 were optimal conditions for the highest ALA yield of 4.5 ± 0.5 mg/g-biomass. Under these conditions, COD removal and biomass production rate were 93.3 ± 0.9% and 31.8 ± 0.5 mg/l/h, respectively. In addition, trace elements Fe2+, Mg2+, Ni2+, and Zn2+ further improved the ALA yield, COD removal, and biomass production rate. Specifically, the highest ALA yield (12.5 ± 0.6 mg/g-biomass) was achieved with Fe2+ addition.

Abstract

This study aimed to optimize four factors affecting biomass accumulation and 5-aminolevulinic acid (ALA) yield together with pollutants removal in Rhodobacter sphaeroides wastewater treatment. Results showed that it was feasible to produce biomass and ALA in R. sphaeroides wastewater treatment. Microaerobic, 1,000-3,000 lux, and pH 7.0 were optimal conditions for the highest ALA yield of 4.5 ± 0.5 mg/g-biomass. Under these conditions, COD removal and biomass production rate were 93.3 ± 0.9% and 31.8 ± 0.5 mg/l/h, respectively. In addition, trace elements Fe2+, Mg2+, Ni2+, and Zn2+ further improved the ALA yield, COD removal, and biomass production rate. Specifically, the highest ALA yield (12.5 ± 0.6 mg/g-biomass) was achieved with Fe2+ addition.

참고문헌 (33)

  1. Carmichael W., "Cyanobacteria secondary metabolites -the cyanotoxins", J. Appl. Bacteriol, 72, pp.445~459 (1992)
  2. Choi HP, "Cloning, expression, and characterization of 5-aminolevulinic acid synthase from Rhodopseudomonas palustris KUGB306", FEMS Microbiol. Lett, 236, pp.175~181 (2004)
  3. Choorit W, "Production of biomass and extracellular 5-aminolevulinic acid by Rhodopseudomonas palustris KG31under light and dark conditions using volatile fatty acid", J. Biosci. Bioeng, 111, pp.658~664 (2011)
  4. Chung S-Y, "Influence of culture conditions on the production of extracellular 5-aminolevulinic acid (ALA) by recombinant E. coli", Process Biochem, 40, pp.385~394 (2005)
  5. Clesscerl LS, "Standard Methods for the Examination of Water and Wastewater", APHA-AWWA-WEF, ~ (1998)
  6. Dubois DY, "A C-truncated glutamyl-tRNA synthetase specific for tRNAGlu is stimulated by its free complementary distal domain: mechanistic and evolutionary implications", Biochemistry, 48, pp.6012~6021 (2009)
  7. Freist W, "Glutamyl-tRNA sythetase", Biol. Chem, 378, pp.1313~1329 (1997)
  8. Hunter CN, "The Purple Phototrophic Bacteria" (2008), Springer
  9. Ishii K, "Light-dependent porphyrin production by suspended and immobilized cells of Rhodobacter sphaeroides", J. Ferment. Bioeng, 69, pp.26~32 (1990)
  10. Jeya M, "Current state of coenzyme Q10 production and its applications", Appl. Microbiol. Biotechnol, 85, pp.1653~1663 (2010)
  11. Kamiyama H, "Production of 5-aminolevulinic acid by a mutant strain of a photosynthetic bacteria", Seibutu Kougaku, 78, pp.48~55 (2000)
  12. Kang Z, "Recent advances in microbial production of δ-aminolevulinic acid and vitamin B12", Biotechnol. Adv, 30, pp.1533~1542 (2012)
  13. Kien NB, "Coenzyme Q10production in a 150-L reactor by a mutant strain of Rhodobacter sphaeroides", J. Ind. Microbiol. Biotechnol, 37, pp.521~529 (2010)
  14. Kuo FS, "Effects of light sources on growth and carotenoid content of photosynthetic bacteria Rhodopseudomonas palustris", Bioresour. Technol, 113, pp.315~318 (2012)
  15. Liu B-F, "The effect of Ni2+, Fe2+ and Mg2+ concentration on photo-hydrogen production by Rhodopseudomonas faecalis RLD-53", Int. J. Hydrogen Energy, 34, pp.721~726 (2009)
  16. Liu XX, "D-Glucose enhanced 5-aminolevulinic acid production in recombinant Escherichia coli culture", Appl. Biochem. Biotechnol, 160, pp.822~830 (2010)
  17. Liu S, "Microbial production and applications of 5-aminolevulinic acid", Appl. Microbiol. Biotechnol, 98, pp.7349~7357 (2014)
  18. Lorrungruang C, "Selection of photosynthetic bacterium Rhodobacter sphaeroides 14F for polyhydroxyalkanoate production with twostage aerobic dark cultivation", J. Biosci. Bioeng, 102, pp.128~131 (2006)
  19. Lu H, "Influences of light and oxygen conditions on photosynthetic bacteria macromolecule degradation: different metabolic pathways", Bioresour. Technol, 102, pp.9503~9508 (2011)
  20. Madukasi EI, "Potentials of phototrophic bacteria in treating pharmaceutical wastewater", Int. J. Environ. Sci. Technol, 7, pp.165~174 (2010)
  21. Nitai B, "The prospect of purple nonsulfur (PNS) photosynthetic bacteria for hydrogen production:the present state of the art", World J. Microbiol. Biotechnol, 23, pp.31~42 (2007)
  22. Ponsano EHG, "Phototrophic growth of Rubrivivax gelatinosus in poultry slaughterhouse wastewater", Bioresour. Technol, 99, pp.3836~3842 (2008)
  23. Sasikala C, "5-Aminolevulinic acid: a potential herbicide", insecticide from microorganisms, 1994, pp.10, 451~459
  24. Sasaki K, "Biosynthesis, biotechnological production and applications of 5-aminolevulinic acid", Appl. Microbiol. Biotechnol, 58, pp.23~29 (2002)
  25. Sasaki K, "Production of a herbicide, 5-aminolevulinic acid, by Rhodobacter sphaeroides using the effluent of swine waste from an anaerobic digestor", Appl. Microbiol. Biotechnol, 32, pp.727~731 (1990)
  26. Sasaki K, "Bioconversion of Waste Materials to Industrial Products", pp.247~291 (1998), Blackie Academic and Professional
  27. Sasaki K, "Influence of iron on the excretion of 5-aminolevulinic acid by a photosynthetic bacterium, Rhodobacter sphaeroides", J. Ferment. Bioeng, 68, pp.378~381 (1989)
  28. Sasaki K, "Effect of culture pH on the extracellular production of 5-aminolevulinic acid by Rhodobacter sphaeroides from volatile fatty acids", Biotechnol. Lett, 15, pp.859~864 (1993)
  29. Sekine S-I, "ATP binding by glutamyl-tRNA synthetase is switched to the productive mode by tRNA binding", EMBO J, 22, pp.676~688 (2003)
  30. Shi X, "Response surface analysis on the effect of cell concentration and light intensity on hydrogen production by Rhodopseudomonas capsulate", Process Biochem, 40, pp.2475~2481 (2005)
  31. Tangprasittipap A, "Biosynthesis of intracellular 5-aminolevulinic acid by a newly identified halotolerant Rhodobacter sphaeroides", Biotechnol. Lett, 29, pp.773~778 (2007)
  32. Wu P, "Effects of Fe2+concentration on biomass accumulation and energy metabolism in photosynthetic bacteria wastewater treatment", Bioresour. Technol, 119, pp.55~59 (2012)
  33. Zhang C, "Effects of mass transfer and light intensity on substrate biological degradation by immobilized photosynthetic bacteria within an annular fiber-illuminating biofilm reactor", J. Photochem. Photobiol. B, 131, pp.113~119 (2014)

관련 논문AI추천

Rhodobacter capsulatus hemA 유전자 발현 대장균에 의한 5-Aminolevulinic Acid 생산의 최적화현영, 강대경, 김민정, 박문원, 박찬수, 신유리, 양동수, 임수진한국미생물·생명공학회 · 한국미생물·생명공학회지2009 · 제37권 제2호Sequential Bioprocesses for Biovalorization of Shrimp Pond Sludge by Hydrolytic Enzymes-Producing Bacterial Consortia and Photosynthetic BacteriaBillateh Asma, Cherisilp Benjamas, Maneechote Wageeporn, Srinuanpan Sirasit, Thongsongkaew Chutema한국미생물·생명공학회 · Journal of Microbiology and Biotechnology2025 · 제35권 A simple culture technique of Rhodobacter azotoformans EBN-7 for public use: application to NH4 +-N removal in shrimp aquaculture water김중균, Kyoung Sook Cho한국수산과학회 · Fisheries and Aquatic Sciences2022 · 제25권 제10호Effects of pH and Light Irradiation on Coenzyme Q10 Production Using Rhodobacter sphaeroides김중균, 정수경, Ngyuen Kien, Van Thingoc Dao한국수산과학회 · Fisheries and Aquatic Sciences2008 · 제11권 제4호Enhancement of Scenedesmus sp. LX1 Biomass Production and Lipid Accumulation Using Iron in Artificial Wastewater and Domestic Secondary EffluentHong-Ying Hu, Jun-Yi Yu, Wen-Yu Zhao, Yin-Hu Wu, Yu Hong한국미생물·생명공학회 · 한국미생물·생명공학회지2014 · 제42권 제2호폐글리세롤을 탄소원으로 hemA와 otsBA를 공동 발현하는 재조합 대장균 배양을 통한 아미노레블린산 생산강대경, 김성배, 김창준, 염정미, 디엡느구팜한국미생물·생명공학회 · 한국미생물·생명공학회지2016 · 제44권 제3호

이 간행물의 다른 논문 (16건)