반응 표면 분석법을 사용한 Rhodobacter sphaeroides PS-24 유래 carotenoid 생산 배지 최적화
Optimization of Medium for the Carotenoid Production by Rhodobacter sphaeroides PS-24 Using Response Surface Methodology
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초록
Response Surface Methodology (RSM), which is combining with Plackett–Burman design and Box-Behnken experimental design, was applied to optimize the ratios of the nutrient components for carotenoid production by Rhodobacter sphaeroides PS-24 in liquid state fermentation. Nine nutrient ingredients containing yeast extract, sodium acetate, NaCl, K2HPO4, MgSO4, mono-sodium glutamate, Na2CO3, NH4Cl and CaCl2 were finally selected for optimizing the medium composition based on their statistical significance and positive effects on carotenoid yield. Box- Behnken design was employed for further optimization of the selected nutrient components in order to increase carotenoid production. Based on the Box-Behnken assay data, the secondary order coefficient model was set up to investigate the relationship between the carotenoid productivity and nutrient ingredients. The important factors having influence on optimal medium constituents for carotenoid production by Rhodobacter sphaeroides PS-24 were determined as follows: yeast extract 1.23 g, sodium acetate 1 g, NH4Cl 1.75 g, NaCl 2.5 g, K2HPO4 2 g, MgSO4 1.0 g, mono-sodium glutamate 7.5 g, Na2CO3 3.71 g, NH4Cl 3.5g, CaCl2 0.01 g, per liter. Maximum carotenoid yield of 18.11 mg/L was measured by confirmatory experiment in liquid culture using 500 L fermenter.
Abstract
Response Surface Methodology (RSM), which is combining with Plackett–Burman design and Box-Behnken experimental design, was applied to optimize the ratios of the nutrient components for carotenoid production by Rhodobacter sphaeroides PS-24 in liquid state fermentation. Nine nutrient ingredients containing yeast extract, sodium acetate, NaCl, K2HPO4, MgSO4, mono-sodium glutamate, Na2CO3, NH4Cl and CaCl2 were finally selected for optimizing the medium composition based on their statistical significance and positive effects on carotenoid yield. Box- Behnken design was employed for further optimization of the selected nutrient components in order to increase carotenoid production. Based on the Box-Behnken assay data, the secondary order coefficient model was set up to investigate the relationship between the carotenoid productivity and nutrient ingredients. The important factors having influence on optimal medium constituents for carotenoid production by Rhodobacter sphaeroides PS-24 were determined as follows: yeast extract 1.23 g, sodium acetate 1 g, NH4Cl 1.75 g, NaCl 2.5 g, K2HPO4 2 g, MgSO4 1.0 g, mono-sodium glutamate 7.5 g, Na2CO3 3.71 g, NH4Cl 3.5g, CaCl2 0.01 g, per liter. Maximum carotenoid yield of 18.11 mg/L was measured by confirmatory experiment in liquid culture using 500 L fermenter.
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