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Estradiol-17β의 복강주사에 따른 미성숙 조피볼락, Sebastes schlegeli의 혈장 VTG, ALPP, Ca, GPT 및 HSI의일시적 변동

Temporal Changes of Plasma VTG, ALPP, Calcium , Glutamate GPT and HSI in the Estradiol-17β-Administered Immature Rockfish, Sebastes schlegeli

심정민|지정훈|박승윤|황운기|강주찬
수산생명의학회 (Aquatic Life Medical Science)|2004.12|한국어병학회지 (JOURNAL OF FISH PATHOLOGY)|제17권 제3호|pp. 191-198

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초록

Estradiol-17β (E2)에 노출된 미성숙 조피볼락의 혈장 내 vitellogenin (VTG), alkaline-labile protein phosphorus (ALPP), calcium (Ca), glutamate pyruvate transaminase (GPT) 및 hepatosomatic index (HSI)의 일시적인 변화가 조사되었다. 70% 에탄올에 녹여진 E2 (5 ㎎/kg B.W.)를 조피볼락 치어의 복강에 주사한 후 0, 1, 3, 6, 9, 12 및 15일에 혈장이 수집되었다. 전기 영동상에서 주사 후, 3일째 약 170 kDa의 위치에 염색성을 나타내는 VTG 밴드가 검출되었다. 밴드의 염색성은 6일째 더욱 강하게 나타났지만, 시간의 경과와 더불어 흐려져 15일째에는 노출 전 처럼 VTG 밴드가 관찰되지 않았다. 혈장 내 ALPP와 Ca는 1일째부터 급격히 증가했으며 VTG 변동과 유사하게 6일째 가장 높은 농도를 나타낸 후, 시간의 경과와 더불어 점점 감소해 15일째는 노출 전과 유사한 값을 나타내었다. GPT는 투여 1일부터 급격히 증가해 3일에 최고치를 나타낸 이후 시간의 경과와 더불어 감소하는 경향을 나타냈으며 HSI의 경우도 1일째부터 증가해 3일째에 가장 높은 수치를 나타낸 후, 점점 감소해 E2 투여전과 유사한 수치를 나타내었다. 이들 결과로 E2에 노출 된 이후 조피볼락의 혈장 내 ALPP, Ca, GPT 및 HSI는 혈장 VTG와 유사한 증감을 나타내는 것을 알 수 있었다. 따라서, ALPP, Ca, GPT 및 HSI는 VTG와 더불어 연안 생태계 내에서 외인성 E2 노출에 대한 생물학적 지표로서 사용 가능할 것으로 판단되어진다.

Abstract

Temporal changes of plasma vitellogenin (VTG), alkaline-labile protein phosphorus (ALPP), calcium (Ca), glutamate pyruvate transaminase (GPT) and hepatosomatic index (HSI) were examined in the estradiol-17β (E2)-administered immature rockfish, Sebastes schlegeli. Fish were intraperitoneally injected with E2 (5 ㎎/kg B.W.) in 70% ethanol and then plasma were extracted at 0, 1, 3, 6, 9, 12 and 15 days. VTG band was detected at a molecular weight position of about 170 kDa on Day 3 in SDS-PAGE. This band became more distinct at 6 days but its was gradually thinned with time-course, and not detected at 15 days. Plasma ALPP and Ca increased suddenly at 1 day and the highest concentrations were detected at 6 days and then these concentrations decreased gradually with time-course. ALPP and Ca concentrations at 15 days after E2 administration were very similar to that before E2 administration. GPT was increased at 1 day and higher GPT was detected at 3 days. However, GPT was gradually decreased with time-course. GPT and HSI at 15 days after E2 administration were also very similar to that before E2 administration. HSI was also increased at 1 day and the highest value was detected at 3 days and then gradually decreased with time-course. These results suggest that plasma ALPP, Ca, GPT and HSI could be utilized as a biomarker of exogenous E2 exposure in coastal ecosystem, because the changes of ALPP, Ca, GPT and HSI after E2 administration are very similar to that of VTG.

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Aluminum Inhibits Vitellogenin Production via Toxic Effects on Hepatocytes in the Rockfish Sebastes schlegelii이윤, 강한승, 손재경, 황운기한국수산과학회 · Fisheries and Aquatic Sciences2011 · 제14권 제4호Effects of 4-Nonlyphenol Exposure on P. olivaceus and S. schlegeli Vitellogenesis정지현, Chang Hee Han, Dae-Jung Kim, Kyong Lan Baeck, Sun Hae Bae, Won Joon Shim한국발생생물학회 · 발생과 생식2012 · 제16권 제1호외인성 성스테로이드 호르몬에 의한 조피볼락, Sebastes schlegeli의 성분화 교란권준영, 김대중, 김상훈, 김주영, 변순규, 이찬희, 임한규, 한형균한국발생생물학회 · 발생과 생식2006 · 제10권 제4호쏨뱅이(Sebastiscus marmoratus)의 Vitellogenin 분석을 위한 효소면역측정법(ELISA) 및 면역크로마토그래피분석법(ICG) 개발여인규, 임윤규한국수산과학회 · 한국수산과학회지2015 · 제48권 제4호조피볼락(Sebastes schlegeli)의 Retinol-Binding Protein의 유전자 발현에 미치는 4-Nonylphenol의 영향김명희, 이재용, 정지현, 조형구, 한창희한국발생생물학회 · 발생과 생식2006 · 제10권 제3호Bisphenol A의 노출에 따른 조피볼락 (Sebastes schlegeli)의 혈액학적 영향강주찬, 금유화, 박수일, 이옥현, 지정훈수산생명의학회 · 한국어병학회지2005 · 제18권 제3호

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