본문 바로가기
KERD
논문KCI 등재

Reponses of the Hepatic Microsomal Cytochrome P450 Monooxygenase System in Rock Bream Oplegnathus fasciatus Exposed to Tributyltin (TBT)

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류수산학

초록

The study was conducted to investigate the responses of the hepatic microsomal cytochrome P450 monooxygenase system in the rock bream Oplegnathus fasciatus after chronic exposure to 0, 1, 2, 4, and 8 μg/L tributyltin (TBT) concentrations for 4 weeks. Hepatic cytochrome 450 content and ethoxyresorufin O-deethylation (EROD) activity were found to significantly increase in fish treated with the higher concentration of TBT (≥4 μg/L); however, no significant changes were observed in penthoxyresorufin Odeethylation (PROD) activity in all treated groups compared to the control group. These findings suggest that exposure to a low TBT concentration (≥4 μg/L) has the potential to induce cytochrome 450 content and EROD enzyme activity in hepatic tissue inthe rock bream.

Abstract

The study was conducted to investigate the responses of the hepatic microsomal cytochrome P450 monooxygenase system in the rock bream Oplegnathus fasciatus after chronic exposure to 0, 1, 2, 4, and 8 μg/L tributyltin (TBT) concentrations for 4 weeks. Hepatic cytochrome 450 content and ethoxyresorufin O-deethylation (EROD) activity were found to significantly increase in fish treated with the higher concentration of TBT (≥4 μg/L); however, no significant changes were observed in penthoxyresorufin Odeethylation (PROD) activity in all treated groups compared to the control group. These findings suggest that exposure to a low TBT concentration (≥4 μg/L) has the potential to induce cytochrome 450 content and EROD enzyme activity in hepatic tissue inthe rock bream.

참고문헌 (31)

  1. Bradford M, "A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding", Anal Biochem, 72, pp.248~245 (1976)
  2. Brack W, "Effect-directed fractionation and identification of cytochrome P450A-inducing halogenated aromatic hydrocharbons in a contaminated sediment", Environ Toxicol Chem, 21, pp.2654~2662 (2002)
  3. Brown JF, "Metabolic alteration of PCB residues in aquatic fauna: distributions of cytochrome P4501A1- and P4502B-like activities", Mar Environ Res, 34, pp.261~266 (1992)
  4. Byrne P, "Effects of a cationic detergent on the gills and blood chemistry of rainbow trout Salmo gairdneri", Dis Aquat Org, 6, pp.195~196 (1989)
  5. Collier TK, "A field evaluation of cytochrome P4501A as a biomarker of contaminant exposure in three species of flat fish", Toxicol Chem, 14, pp.143~152 (1995)
  6. Diez S, "Organotin contamination in sediments from the Western Mediterranean enclosures following 10 years of TBT regulation", Water Res, 36, pp.905~918 (2002)
  7. Fent K, "Inhibition of hepatic microsomal monooxygenase system by organotins in vitro in freshwater fish", Aquat Toxicol, 28, pp.107~126 (1994)
  8. Fent K, "Tributyltin-induced effects on early life stages of minnows, Phoxinus phoxinus", Arch Environ contam Toxicol, 22, pp.429~438 (1992)
  9. Gibbs PE, "Sex change in the female dog-whelk, Nucella lapillus around southwest England: Evidence for the effect of tributyltin from antifouling paints", J Mar Biol Assoc UK, 68, pp.715~731 (1988)
  10. Goksøyr A, "Use of cytochrome P450 1A (CYP1A) in fish as a biomarker of aquatic pollution", Arch Toxicol, 17, pp.80~95 (1995)
  11. Goksøyr A, "The cytochrome P450 system in fish, aquatic toxicology and environmental monitoring", Aquat Toxicol, 22, pp.287~312 (1992)
  12. Haasch ML, "Cloned rainbow trout liver P450 complementary DNA as a potential environmental monitor", Toxicol Appl Pharmacol, 98, pp.362~368 (1989)
  13. Horiguchi T, "Organotin compounds and their effects on aquatic organisms, focusing on imposex in gastropods", Main Group Metal Chem, 17(1-4), pp.81~100 (1994)
  14. Kennedy SW, "Simultaneous measurement of cytochrome P4501A catalytic activity and total protein concentration with a fluorescence plate", Anal. Biochem, 222, pp.217~223
  15. Livingstone DR, "Aquatic environmental biomonitoring: Use of cytochrome P450 1A and other molecular biomarkers in fish and mussels, In Environmental Biomonitoring: The Biotechnology Ecotoxicology Interface Vol 6-Biotechnology Reserch Series", pp.101~129 (1998), Cambridge University Press
  16. Lu AYH, "Multiplicity of mammalian microsomal cytochrome P-450", Pharmacological Reviews, 31, pp.277~295 (1980)
  17. Mc Allister B, "Early life exposure to environmental levels of the aromatase inhibitor tributyltin causes masculinisation and irreversible sperm damage in zebrafish (Danio rerio)", Aquat Toxicol, 65, pp.309~316 (2003)
  18. Morcillo Y, "Interaction of tributyltin- and triphenyltin with the microsomal monooxygenase system of mollusks and fish from the NW Mediterranean", Aquat Toxicol, 38, pp.35~46 (1997)
  19. Oehlmann J, "Imposex in Nucella lapillus and intersex in Littorina littorea: interspecific comparison of two TBT-induced effects and their geographical uniformity", Hydrobiologia, 378, pp.199~213 (1998)
  20. Omura T, "The carbon monoxide-binding pigment of liver microsomes. 2. Solubilization, purification and properties", J Biol Chem, 239, pp.2379~2385 (1964)
  21. Ric CD, "Tributyltin modulates 3,3’,4,4’,5-pentachlorobiphenyl (PCB-126)-induced hepatic CYP1A activity in channel catfish, Ictalurus punctatus", Journal of Toxicology and Environmental Health, 55, pp.197~212 (1998)
  22. Schwaiger J, "A prolonged toxicity study on the effects of sublethal concentration of bis (tri-n-butyltin) oxide (TBTO): histopathological and histochemical findings in rainbow trout (Oncorhynchus mykiss)", Aquat Toxicol, 23, pp.31~48 (1992)
  23. Skaare JU, "Response of xenobiotic metabolizing enzymes of rainbow trout (Oncorhynchus mykiss) to the mono-ortho substituted polychlorinated PCB congener 2, 3’, 4, 4’, 5-pentachlorobiphenyl, PCB-118, detected by enzyme activities and immunochemical methods", Arch Environ Contam Toxicol, 20, pp.349~352 (1991)
  24. Stegeman JJ, "Biochemistry and molecular biology of monooxygenases: Current perspectives on forms, functions, and regulation of cytochrome P450 in aquatic species, In Aquatic Toxicology Molecular Biochemical and Cellular Perspectives", pp.87~206 (1994), Lewis
  25. Stegeman JJ, "Structure, function and regulation of cytochrome P450 forms in fish", Biochem Soc Trans, 18, pp.19~21 (1990)
  26. Tsuda T, "Accumulation and excretion of tri-n-butyltin chloride and tributyltin chloride by willow shiner", Comp Biochem Physiol, 101C, pp.67~70 (1992)
  27. Wang DY, "Toxic effects of tributyltin (TBT) on early life stage of thornfish (Terapon jarbua Forsskål)", J Fish Soc Taiwan, 25, pp.15~25 (1998)
  28. Whyte JJ, "Ethoxyresorufin-Odeethylase (EROD) activity in fish as a biomarker of chemical exposure", Crit Rev Toxicol, 30, pp.347~570 (2000)
  29. Wrighton SA, "The human hepatic cytochrome P-450 involved in drug metabolism", Crit Rev Toxicol, 22, pp.1~22 (1992)
  30. Xie J, "Effects of anthraquinone extract from rhubarb Rheum officinal Bail on the crowding stress response and growth of common carp Cyprinus carpio var. Jian", Aquaculture, 281, pp.5~11 (2008)
  31. Yolanda M, "Interaction of tributylyin with hepatic cytovhrome P450 and uridine diphosphate-glucuronosyl tranfetase system of fish", Environ Toxicol and Chem, 23(4), pp.990~996 (2004)

관련 논문AI추천

이 간행물의 다른 논문 (14건)