Effect of Astragalus Membranaceus Extract against Improvement of Myelosuppression and Quality of Life in 5-Fluorouracil Treated Mice
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목 적 : 황기추출물이 생쥐의 5-FU로 유발된 골수억제와 삶의 질 저하의 개선에 미치는 효과를 연구하고자한다.방 법 : 골수억제에 미치는 영향의 평가를 위해 Complete Blood Count, Histological Analysis of BM, Cell Colony Forming Assay for Hematopoietic Progenitor를 시행하고, 삶의 질에 미치는 영향을 평가하기 위해 Swimming Test, Survival Rate, Nitric Oxide (NO) Assay, 51Cr Release Assay in NK Cell, mRNA Expressions of IL-1β, IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, TGF-β and GM-CSF in Spleen Cells를 시행하였다.결 과 : 황기추출물은 골수억제를 호전시켜 말초혈액수치를 회복시키고, 골수파괴를 보호하는 효과를 보이며, 대조군에 비해 혈구생성을 촉진시키며, 대조군에 비해 생존율과 수영시간을 증가시키며, 대식세포와 자연살상세포의 활동성을 증가시키며, 종양면역과 관련된 싸이토카인(IL-2, IL-6, IFN-γ)의 발현을 증가시켰다.결 론 : 5-FU로 유발된 골수억제와 삶의 질 저하의 개선에 미치는 황기추출물의 유용성이 기대되며 향후 이에 대한 지속적인 연구가 필요할 것으로 사료된다.
Abstract
Objective : To evaluate the effect of Astragalus Membranaceus Extract (AME) on myelosuppression, activity and immune modulation in 5-fluorouracil (5-FU) treated mice. Method : We carried out complete blood count, histological analysis of bone marrow, and cell colony forming assay for hematopoietic progenitor to evaluate the effect of AME on myelosuppression and conducted swimming test, survival rate, nitric oxide (NO) assay , 51Cr release assay in natural killer cell, mRNA expressions of IL-1β, IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, TGF-β and GM-CSF in spleen cells to evaluate the effect of AME on quality of life (QOL). Results : AME improved 5-FU induced myelosuppression and peripheral blood count was recovered effectively, had significant efficacy to protect against chemotherapy induced marrow-destruction and on hematopoiesis compared with the control group, improved increase survival rate and the swimming time, had a stimulatory effect on macrophage activation and NK cell activity, and up-regulated cytokine gene transcription (IL-2, IL-6, IFN-γ) in murine immunologic system. Conclusion : We can conclude that AM is an effective herbal agent for improvement of myelosuppression and QOL in 5-FU treated mice.
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