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地骨皮가 H₂O₂에 의한 LLC-PK₁ 세포의 Redox Status 및 NF-κB Signaling에 미치는 영향

The Effects of Lycium chinense Milie on The H₂O₂-treated LLC-PK₁ Cell's Redox Status and NF-κB Signaling

신현철|최규호
대한한방내과학회 (The Society Of Korean Oriental Internal Medicine)|2009.03|대한한방내과학회지 (The Journal of Korean Oriental Internal Medicine)|제30권 제1호|pp. 36-50

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참고문헌

키워드

NONF-κBiNOSCOX-2H₂O₂Lycium chinense MilieLLC-PK1 cellO₂⁻ONOO⁻PGE₂
주제분류한의학

초록

Objectives : This study was aimed to verify the cytoprotective function, antioxidative effect and inflammation genes inhibitory effects of Lycium chinense Milie. Therefore the generation of superoxide anion radical (O₂⁻), peroxynitrite (ONOO⁻), nitric oxide (NO) and Prostaglandin E₂ (PGE₂) was investigated in the renal epitheial cells of mouse. And effects of Lycium chinense Milie on the expression of inflammation-related proteins, IKK-α, p-IKK-αβ, p-IκB-α, NF-κB (p50, p65), COX-2 and iNOS, were examined by western blotting. Methods : For this study, the fluorescent probes, namely dihyldrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed using anti-IKK-α, anti-phospho IKK-αβ, anti-phospho IκB-α, anti-NF-κB (p50, p65), anti-COX-2 and anti-iNOS, respectively. Results : Lycium chinense Milie reduced H₂O₂-induced cell death dose-dependently. It inhibited the generation of ․O₂⁻, ONOO⁻, NO and PGE₂ in the H₂O₂-treated renal epitheial cells of mouse in vitro. Lycium chinense Milie inhibited the expression of IKK-α, p-IKK-αβ, p-IκB-α, COX-2 and iNOS genes by means of decreasing activation of NF-κB. Conclusions : According to above results, Lycium chinense Milie was expected to be applied to the treatment for the inflammatory process and inflammation-related diseases.

Abstract

Objectives : This study was aimed to verify the cytoprotective function, antioxidative effect and inflammation genes inhibitory effects of Lycium chinense Milie. Therefore the generation of superoxide anion radical (O₂⁻), peroxynitrite (ONOO⁻), nitric oxide (NO) and Prostaglandin E₂ (PGE₂) was investigated in the renal epitheial cells of mouse. And effects of Lycium chinense Milie on the expression of inflammation-related proteins, IKK-α, p-IKK-αβ, p-IκB-α, NF-κB (p50, p65), COX-2 and iNOS, were examined by western blotting. Methods : For this study, the fluorescent probes, namely dihyldrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2',7'-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed using anti-IKK-α, anti-phospho IKK-αβ, anti-phospho IκB-α, anti-NF-κB (p50, p65), anti-COX-2 and anti-iNOS, respectively. Results : Lycium chinense Milie reduced H₂O₂-induced cell death dose-dependently. It inhibited the generation of ․O₂⁻, ONOO⁻, NO and PGE₂ in the H₂O₂-treated renal epitheial cells of mouse in vitro. Lycium chinense Milie inhibited the expression of IKK-α, p-IKK-αβ, p-IκB-α, COX-2 and iNOS genes by means of decreasing activation of NF-κB. Conclusions : According to above results, Lycium chinense Milie was expected to be applied to the treatment for the inflammatory process and inflammation-related diseases.

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