楡根皮 추출물의 활성종 억제 및 염증 촉진 인자 제어 효과
The Inhibitory Effects of Ulmus davidiana on the Reactive Species and Proinflammatory Proteins
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Objectives : This study was to investigate the inhibitory effects of Ulmus davidiana on the generation of peroxynitrite (ONOO-), nitric oxide (NO) and superoxide anion radicals (O2-) in the endothelial cells of rat vessels. The effects of Ulmus davidiana on the expression of inflammation-related proteins, NF-κB (p50, p65), COX-2, and iNOS, were examined by western blotting.Methods : For this study, fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed via using anti-NF-κB (p50, p65), anti-COX-2, and anti-iNOS, respectively.Results : Ulmus davidiana inhibited the generation of ONOO-, NO and O2- in the lipopolysaccharide (LPS)-treated endothelial cells of rat vessels in vitro. Ulmus davidiana inhibited the expression of COX-2 and iNOS genes by means of decreasing the NF-κB activation.Conclusions : These results suggest Ulmus davidiana is effective on inhibiting the generation of ONOO-, NO and O2-, and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related diseases.
Abstract
Objectives : This study was to investigate the inhibitory effects of Ulmus davidiana on the generation of peroxynitrite (ONOO-), nitric oxide (NO) and superoxide anion radicals (O2-) in the endothelial cells of rat vessels. The effects of Ulmus davidiana on the expression of inflammation-related proteins, NF-κB (p50, p65), COX-2, and iNOS, were examined by western blotting.Methods : For this study, fluorescent probes, namely dihydrorhodamine 123 (DHR 123), 4,5-diaminofluorescein (DAF-2) and 2’,7’-dichlorodihydrofluorescein diacetate (DCFDA) were used. Western blotting was performed via using anti-NF-κB (p50, p65), anti-COX-2, and anti-iNOS, respectively.Results : Ulmus davidiana inhibited the generation of ONOO-, NO and O2- in the lipopolysaccharide (LPS)-treated endothelial cells of rat vessels in vitro. Ulmus davidiana inhibited the expression of COX-2 and iNOS genes by means of decreasing the NF-κB activation.Conclusions : These results suggest Ulmus davidiana is effective on inhibiting the generation of ONOO-, NO and O2-, and that therefore it might have a potential role as a treatment for the inflammatory process and inflammation-related diseases.
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