Protein variation and involvement of insulin-like growth factor during embryonic development in the olive flounder Paralichthys olivaceus
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Insulin-like growth factors (IGFs), along with IGF-binding protein and IGF receptor, are well-known regulators in the growth and survival of vertebrates. In this study, we investigated the involvement of IGFs and protein variation during embryonic development of the olive flounder (Paralichthys olivaceus). Morphological stages were divided into six main developments as blastula, gastrula, cephalization, cranial regionalization, tail lift, and hatch. During embryonic development, protein variation was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrospray ionization quadrupole time-of-flight mass spectrometry/mass spectrometry. In addition, the mechanism of signaling of IGF-I receptor was examined using immuno-blot analysis. We found marked changes in protein expression at four stages of embryonic development and identified proteins as belonging to the vitellogenin 2 family. As development progresses, expression of IGF-II, phosphotyrosine, and phospho-Akt increased, while expression of growth factor receptor-bound protein 2 (GRB2) and one of guanine-nucleotide-binding proteins (Ras) decreased. These results provide basic information on the IGF system in the embryonic development of the olive flounder.
Abstract
Insulin-like growth factors (IGFs), along with IGF-binding protein and IGF receptor, are well-known regulators in the growth and survival of vertebrates. In this study, we investigated the involvement of IGFs and protein variation during embryonic development of the olive flounder (Paralichthys olivaceus). Morphological stages were divided into six main developments as blastula, gastrula, cephalization, cranial regionalization, tail lift, and hatch. During embryonic development, protein variation was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrospray ionization quadrupole time-of-flight mass spectrometry/mass spectrometry. In addition, the mechanism of signaling of IGF-I receptor was examined using immuno-blot analysis. We found marked changes in protein expression at four stages of embryonic development and identified proteins as belonging to the vitellogenin 2 family. As development progresses, expression of IGF-II, phosphotyrosine, and phospho-Akt increased, while expression of growth factor receptor-bound protein 2 (GRB2) and one of guanine-nucleotide-binding proteins (Ras) decreased. These results provide basic information on the IGF system in the embryonic development of the olive flounder.
참고문헌 (24)
- Caelers A, "Establishment of a real-time RT-PCR for the determination of absolute amounts of IGF-I and IGF-II gene expression in liver and extrahepatic sites of the tilapia", Gen Comp Endocrinol, 137, pp.196~204 (2004)[DOI]
- Chandra V, "Temporal expression pattern of insulin-like growth factors (IGF-1 and IGF-2) ligands and their receptors (IGF-1R and IGF-2R) in buffalo (Bubalus bubalis) embryos produced in vitro", Live Sci, 135, pp.225~230 (2011)[DOI]
- Choi YH, "Gametogenic cycle of the transplanted-cultured pearl oyster, Pinctada fucata martensii (Bivalvia: Pteriidae) in Korea", Aquaculture, 220, pp.781~790 (2003)[DOI]
- Choi YH, "Dietary Hizikia fusiformis glycoproteininduced IGF-I and IGFBP-3 associated to somatic growth, polyunsaturated fatty acid metabolism, and immunity in juvenile olive flounder Paralichthys olivaceus", Comp Biochem Physiol A, 167, pp.1~6 (2014)
- Choi YH, "Chemical and mass spectrometry characterization of the red alga Pyropia yezoensis chemoprotective protein (PYP): protective activity of the N-terminal fragment of PYP1 against acetaminophen-induced cell death in Chang liver cells", Int J Mol Med, 35, pp.271~276 (2015)[DOI]
- Duan C, "The insulin-like growth factor system and its biological actions in fish", Amer Zool, 37, pp.491~503 (1997)[DOI]
- Duan C, "Roles of insulin-like growth factor (IGF) binding proteins in regulating IGF actions", Gen Comp Endocrinol, 142, pp.44~52 (2005)[DOI]
- Emmersen BK, "Natural occurrence, and experimental induction by estradiol-<TEX>$17{\beta}$<", TEX>, of a lipophosphoprotein (vitellogenin) in flounder (Platichtys flesus, L.), 1976, pp.54B, 443~446
- Fuentes EN, "IGF-I", PI3K, MAPK, pp.ERK pathways in vivo in skeletal muscle are regulated by nutrition and contribute to somatic growth in the fine flounder, Am J Physiol Regul Integr Comp Physiol[DOI]
- Hardy K, "Growth factor expression and function in the human and mouse preimplantaion embryo", J Endocrinol, 172, pp.221~236 (2002)[DOI]
- Li M, "The actions of in ovo cortisol on egg fertility, embryo development and the expression of growth-related genes in rainbow trout embryos, and the growth performance of juveniles", Mol Reprod Dev, 77, pp.922~931 (2010)[DOI]
- Li Y, "Expression of insulin-like growth factor-1 of orange-spotted grouper (Epinephelus coioides) in yeast Pichia pastoris", Protein Expr Purif, 84, pp.80~85 (2012)[DOI]
- Manning BD, "PKB signaling: navigating downstream", Cell, 129, pp.1261~1274 (2007)[DOI]
- Martinez GM, "Embryonic and larval staging of summer flounder (Paralichthys dentatus)", J Morphol, 255, pp.162~176 (2003)[DOI]
- Matsubara T, "Two forms of vitellogenin, yielding two distinct lipovitellins, play different roles during oocyte maturation and early development of barfin flounder, Verasper moseri, a marine teleost that spawns pelagic eggs", Dev Biol, 213, pp.18~32 (1999)[DOI]
- Ohlsson C, "The relative importance of endocrine versus autocrine", paracrine insulin-like growth factor-I in the regulation of body growth, 2000, pp.14, 541~543
- Riley JK, "The PI3K", Akt pathway is present and functional in the preimplantation mouse embryo, 2005, pp.284, 377~386
- Santos AN, "Cell lineage-specific signaling of insulin and insulin-like growth factor I in rabbit blastocysts", Endocrinol, 149, pp.515~524 (2008)[DOI]
- Schlueter PJ, "Insulin-like growth factor signaling regulates zebrafish embryonic growth and development by promoting cell survival and cell cycle progression", Cell Death Differ, 14, pp.1095~1105 (2007)[DOI]
- Silversand C, "Isolation of turbot (Scophthalmus maximus) vitellogenin by high-performance anion-exchange chromatography", J Chromatogr, 478A, pp.387~397 (1989)
- Vera Cruz EM, "Insulin-like growth factor-I cDNA cloning, gene expression and potential use as a growth rate indicator in Nile tilapia, Oreochromis niloticus", Aquaculture, 251, pp.585~595 (2006)[DOI]
- Xu Y, "Insulin-like growth factors I and II in starry flounder (Platichthys stellatus): molecular cloning and differential expression during embryonic development", Fish Physiol Biochem, 41, pp.139~152 (2015)[DOI]
- Yuan Y, "Medaka insulin-like growth factor-2 supports self-renewal of the embryonic stem cell line and blastomeres in vitro", Sci Rep, 7, pp.78~ (2017)[DOI]
- Zou S, "Zebrafish IGF genes: gene duplication, conservation and divergence, and novel roles in midline and notochord development", PLoS One, 4, pp.e7026~ (2009)[DOI]