Transcriptional Onset of Lysozyme Genes during Early Development in Olive Flounder (Paralichthys olivaceus)
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The immune system in teleost fish is not completely developed during embryonic and larval stages, thereforeeffective innate mechanisms is very important for survival in such an environment. However, the knowledge of thedevelopment of immune system assumed to be restricted. In many species, lysozymes have been considered as importantgenes of the first line immune defense. The early detection of lysozyme mRNA in previous reports, led to the investigation ofits presence in oocytes. As a result, c-type lysozyme mRNA transcripts were detected in unfertilized oocytes indicatingmaternal transfer. Therefore, we investigated the expression patterns of lysozymes in flounder, including the matured oocyte. In our results, c-type lysozyme mRNA was first detected in unfertilized oocyte stage, observed the significantly decreaseduntil hatching stage, and was significantly increased after hatching stage. On the other hand, g-type lysozyme mRNAtranscripts were first detected at late neurula stage, and the mRNA level was significantly increased after 20 dph. It may besuggest that maternally supplied mRNAs are selectively degraded prior to the activation of embryonic transcription. Thisstudy will be help in understanding the maturation and onset of humoral immunity during development of olive flounderimmune system.
Abstract
The immune system in teleost fish is not completely developed during embryonic and larval stages, thereforeeffective innate mechanisms is very important for survival in such an environment. However, the knowledge of thedevelopment of immune system assumed to be restricted. In many species, lysozymes have been considered as importantgenes of the first line immune defense. The early detection of lysozyme mRNA in previous reports, led to the investigation ofits presence in oocytes. As a result, c-type lysozyme mRNA transcripts were detected in unfertilized oocytes indicatingmaternal transfer. Therefore, we investigated the expression patterns of lysozymes in flounder, including the matured oocyte. In our results, c-type lysozyme mRNA was first detected in unfertilized oocyte stage, observed the significantly decreaseduntil hatching stage, and was significantly increased after hatching stage. On the other hand, g-type lysozyme mRNAtranscripts were first detected at late neurula stage, and the mRNA level was significantly increased after 20 dph. It may besuggest that maternally supplied mRNAs are selectively degraded prior to the activation of embryonic transcription. Thisstudy will be help in understanding the maturation and onset of humoral immunity during development of olive flounderimmune system.
참고문헌 (41)
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