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가덕도 연안 해수에서 Vibrio vulnificus의 분포 및 분리균주의 병원성 유전자 특성

Distribution and Molecular Characteristics of Vibrio vulnificus Isolated from Seawater Along the Gadeok Island Coast

정희진|김영목|오희경
한국수산과학회 (The Korean Society of Fisheries and Aquatic Science)|2020.10|한국수산과학회지 (Korean Journal of Fisheries and Aquatic Sciences)|제53권 제5호|pp. 688-693|DOI https://doi.org/10.5657/KFAS.2020.0688

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참고문헌

키워드

Virulence geneVibrio vulnificusvvhAviuBvcgC
주제분류수산학

초록

Vibrio vulnificus is a Gram-negative marine bacterium known to cause septicemia. This study was conducted to investigate the distribution of V. vulnificus along the coast of Gadeok Island in Korea and to determine the molecular characteristics of isolated strains sampled between March and November 2019 from seawater. The strains were mostly detected between July and September, when the average water temperature and average salinity were 22.2-26.2°C and 14.2-29.9 psu, respectively. V. vulnificus was not detected in seawater below 15°C. In September, the highest population of V. vulnificus was observed at 2,100 MPN (most probable number)/100 mL, attributable to decreased salinity from heavy rains. In addition, the detection rate of V. vulnificus was higher at the sampling station near the Nakdong River. Virulence-related genes were also identified among the isolates, such as vvhA (97.1%), viuB (44.1%), and vcgC (57.4%). In particular, viuB and vcgC were only observed in V. vulnificus isolated from June to September, when the detection rate was high and water temperature was above 20°C, suggesting the role of seasonal characteristics.

Abstract

Vibrio vulnificus is a Gram-negative marine bacterium known to cause septicemia. This study was conducted to investigate the distribution of V. vulnificus along the coast of Gadeok Island in Korea and to determine the molecular characteristics of isolated strains sampled between March and November 2019 from seawater. The strains were mostly detected between July and September, when the average water temperature and average salinity were 22.2-26.2°C and 14.2-29.9 psu, respectively. V. vulnificus was not detected in seawater below 15°C. In September, the highest population of V. vulnificus was observed at 2,100 MPN (most probable number)/100 mL, attributable to decreased salinity from heavy rains. In addition, the detection rate of V. vulnificus was higher at the sampling station near the Nakdong River. Virulence-related genes were also identified among the isolates, such as vvhA (97.1%), viuB (44.1%), and vcgC (57.4%). In particular, viuB and vcgC were only observed in V. vulnificus isolated from June to September, when the detection rate was high and water temperature was above 20°C, suggesting the role of seasonal characteristics.

참고문헌 (33)

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