해수 및 수산물에서 분리한 Vibrio vulnificus의 병원성 유전자 및 항균제 내성
Profiles of Virulence Genes and Antimicrobial Resistance of Vibrio vulnificus Strains Isolated from Seawater and Fisheries
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초록
We isolated 28 Vibrio vulnificus strains from seawater and fisheries and investigated the positive rate of eight virulence genes. Additionally, we evaluated the susceptibility of these strains to 25 antimicrobials. The positive rates of fur, vvhA, tcp, rtxA, vcgC, viuB, vvp, and acfA were 100, 92.9, 92.9, 67.9, 64.3, 25.0, 14.3, and 7.1%, respectively. A disk diffusion susceptibility test revealed that, all the investigated strains had the highest resistance to amoxicillin and oxacillin, followed by that to streptomycin (96.4%), cefoxitin (92.9%), clindamycin (82.1%), amikacin (67.9%), vancomycin (46.4%), nalidixic acid (7.1%), penicillin G (7.1%), and ampicillin (3.6%). Moreover, they were susceptible to 10 other antimicrobials, including cefotaxime, chloramphenicol, erythromycin, gentamicin, and rifampicin. Notably, amoxicillin, oxacillin, and streptomycin had average minimum inhibitory concentrations of 132.6, 603.4, and 23.1 μg/mL against V. vulnificus, respectively. These observations provide new insights regarding the necessity for sanitation of commercial fisheries and can potentially, help reduce the risk posed by fisheries contaminated with bacteria resistant to antimicrobials.
Abstract
We isolated 28 Vibrio vulnificus strains from seawater and fisheries and investigated the positive rate of eight virulence genes. Additionally, we evaluated the susceptibility of these strains to 25 antimicrobials. The positive rates of fur, vvhA, tcp, rtxA, vcgC, viuB, vvp, and acfA were 100, 92.9, 92.9, 67.9, 64.3, 25.0, 14.3, and 7.1%, respectively. A disk diffusion susceptibility test revealed that, all the investigated strains had the highest resistance to amoxicillin and oxacillin, followed by that to streptomycin (96.4%), cefoxitin (92.9%), clindamycin (82.1%), amikacin (67.9%), vancomycin (46.4%), nalidixic acid (7.1%), penicillin G (7.1%), and ampicillin (3.6%). Moreover, they were susceptible to 10 other antimicrobials, including cefotaxime, chloramphenicol, erythromycin, gentamicin, and rifampicin. Notably, amoxicillin, oxacillin, and streptomycin had average minimum inhibitory concentrations of 132.6, 603.4, and 23.1 μg/mL against V. vulnificus, respectively. These observations provide new insights regarding the necessity for sanitation of commercial fisheries and can potentially, help reduce the risk posed by fisheries contaminated with bacteria resistant to antimicrobials.
참고문헌 (29)
- Acar JF, "Antibiotics in laboratory medicine", pp.17~52 (1991), Williams and Wilkins
- Nor Zulkiply Amalina, "Prevalence, antimicrobial susceptibility and plasmid profiling of Vibrio spp. isolated from cultured groupers in Peninsular Malaysia", BMC Microbiology, 19(1), pp.251~ (2019), Springer Science and Business Media LLC[DOI]
- Craig Baker-Austin, "Vibrio vulnificus: new insights into a deadly opportunistic pathogen", Environmental Microbiology, 20(2), pp.423~430 (2018), Wiley[DOI]
- Craig Baker-Austin, "Vibrio spp. infections", Nature Reviews Disease Primers, 4(1), pp.1~19 (2018), Springer Science and Business Media LLC[DOI]
- Balows A, "Manual of clinical microbiology", pp.384~395 (1991), ASM Press
- Ryan W. Bogard, "Role of Iron in Human Serum Resistance of the Clinical and Environmental <i>Vibrio vulnificus<", i> Genotypes, 2007, pp.73(23), 7501~7505[DOI]
- Cynthia W. Brasher, "Detection of Microbial Pathogens in Shellfish with Multiplex PCR", Current Microbiology, 37(2), pp.101~107 (1998), Springer Science and Business Media LLC[DOI]
- L A Brauns, "Use of the polymerase chain reaction in detection of culturable and nonculturable Vibrio vulnificus cells", Applied and Environmental Microbiology, 57(9), pp.2651~2655 (1991), American Society for Microbiology[DOI]
- 조의동, "곰소만 및 변산 해역 해수에 병원성 비브리오균(Vibrios spp.)의 분포", 한국수산과학회지, 52(6), pp.625~630 (2019), 한국수산과학회[DOI]
- CLSI(Clinical and Laboratory Standards Institute), "Performance standards for antimicrobial susceptibility testing:20th informational supplement. M100-S20-U", CLSI, ~ (2010)
- Melissa K. Jones, "Vibrio vulnificus: disease and pathogenesis", Infection and Immunity, 77(5), pp.1723~1733 (2009), American Society for Microbiology[DOI]
- Kaspar CW, "Effects of temperature and salinity on the survival of Vibrio vulnificus in seawater and shellfish", Appl Environ Microbiol, 59, pp.2426~2429 (1993)
- "Infectious disease portal" (2021)[URL]
- 김동민, "비브리오 패혈증", 대한내과학회지, 82(6), pp.671~679 (2012), 대한내과학회[DOI]
- Hyun-Joong Kim, "Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics", BMC Microbiology, 15(1), pp.239~ (2015), Springer Science and Business Media LLC[DOI]
- Seung Hun Lee, "Distribution of Pathogenic Vibrio Species in the Coastal Seawater of South Korea (2017–2018)", Osong Public Health and Research Perspectives, 10(6), pp.337~342 (2019), 질병관리청[DOI]
- Xixi Li, "Virulence properties of Vibrio vulnificus isolated from diseased zoea of freshness shrimp Macrobrachium rosenbergii", Microbial Pathogenesis, 127, pp.166~171 (2019), Elsevier BV[DOI]
- Kouichi Matsumoto, "Clinical features of Vibrio vulnificus infections in the coastal areas of the Ariake Sea, Japan", Journal of Infection and Chemotherapy, 16(4), pp.272~279 (2010), Elsevier BV[DOI]
- "Food poisoning statistics" (2021)[URL]
- Motes ML, "Influence of water temperature and salinity on Vibrio vulnificus in Northern gulf and Atlantic coast oysters(Crassostrea virginica)", Appl Environ Microbiol, 64, pp.1459~1465 (1998)
- 오보영, "인천지역에서 분리된 비브리오 패혈증균의 특성", 미생물학회지, 43(4), pp.256~263 (2007), 한국미생물학회
- James D. Oliver, "The biology of Vibrio vulnificus", Microbiology Spectrum, 3(3), pp.1~10 (2015), American Society for Microbiology[DOI]
- Gitika Panicker, "Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays", Applied and Environmental Microbiology, 70(12), pp.7436~7444 (2004), American Society for Microbiology[DOI]
- Gregg S. Pettis, "Structure, Function, and Regulation of the Essential Virulence Factor Capsular Polysaccharide of Vibrio vulnificus", International Journal of Molecular Sciences, 21(9), pp.3259~ (2020), MDPI AG[DOI]
- Thomas M. Rosche, "A Rapid and Simple PCR Analysis Indicates There Are Two Subgroups ofVibrio vulnificusWhich Correlate with Clinical or Environmental Isolation", Microbiology and Immunology, 49(4), pp.381~389 (2005), Wiley[DOI]
- Fabiano L. Thompson, "Biodiversity of Vibrios", Microbiology and Molecular Biology Reviews, 68(3), pp.403~431 (2004), American Society for Microbiology[DOI]
- Ji Hye Yang, "Distribution and antimicrobial susceptibility of Vibrio species associated with zooplankton in coastal area of Korea", Marine Pollution Bulletin, 125(1-2), pp.39~44 (2017), Elsevier BV[DOI]
- 윤연희, "어류, 수족관수 및 환자에서 분리된 Vibrio vulnificus의 독소유전자 분포 및 항생제 내성", 한국식품위생안전성학회지, 35(1), pp.6~12 (2020), 한국식품위생안전성학회[DOI]
- Yuan Yuan, "Vibrio vulnificus Hemolysin: Biological Activity, Regulation of vvhA Expression, and Role in Pathogenesis", Frontiers in Immunology, 11, pp.599439~ (2020), Frontiers Media SA[DOI]