금강 하구 해역의 해수에 병원성 비브리오균(Vibrio spp.)의 분포
Distribution of Pathogenic Vibrio spp. in Seawater of the Geum River Estuary Area, West Coast of Korea
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
The pathogenic Vibrio genus denotes halophilic bacteria that are distributed in aquatic environments, including both sea and freshwater. V. cholerae, V. vulnificus, and V. parahaemolyticus are the main species that can be potent human pathogens and the leading cause of septicemia, wound infections, and seafood borne gastroenteritis. The aim of this study was to investigate the presence of pathogenic Vibrios in seawater. We obtained a total of 80 seawater samples from the Geum River estuary area in the west coast of Korea from April to December 2021. Pathogenic Vibrios was determined using a combination of the most probable number-polymerase chain reaction (MPN-PCR) methods. The detection levels of V. cholerae, V. parahaemolyticus, and V. vulnificus in the seawater samples were 7.5%, 68.8%, and 30.0%, respectively. The quantitative results were as follows: 3.6–3.6 MPN/100 mL in V. cholerae, 3.6–3,400 MPN/100 mL in V. parahaemolyticus, and 3.6–4,300 MPN/100 mL in V. vulnificus. Overall, these results provide novel insight into the necessity for seawater sanitation in the Geum River estuary area, and could help reduce the risk of seafood-borne outbreaks caused by pathogenic Vibrios.
Abstract
The pathogenic Vibrio genus denotes halophilic bacteria that are distributed in aquatic environments, including both sea and freshwater. V. cholerae, V. vulnificus, and V. parahaemolyticus are the main species that can be potent human pathogens and the leading cause of septicemia, wound infections, and seafood borne gastroenteritis. The aim of this study was to investigate the presence of pathogenic Vibrios in seawater. We obtained a total of 80 seawater samples from the Geum River estuary area in the west coast of Korea from April to December 2021. Pathogenic Vibrios was determined using a combination of the most probable number-polymerase chain reaction (MPN-PCR) methods. The detection levels of V. cholerae, V. parahaemolyticus, and V. vulnificus in the seawater samples were 7.5%, 68.8%, and 30.0%, respectively. The quantitative results were as follows: 3.6–3.6 MPN/100 mL in V. cholerae, 3.6–3,400 MPN/100 mL in V. parahaemolyticus, and 3.6–4,300 MPN/100 mL in V. vulnificus. Overall, these results provide novel insight into the necessity for seawater sanitation in the Geum River estuary area, and could help reduce the risk of seafood-borne outbreaks caused by pathogenic Vibrios.
참고문헌 (23)
- Baker-Austin C, "Vibrio spp. infections", Nat Rev Dis Primers, 4, pp.1~19 (2018)[DOI]
- Baker-Austin C, "Vibrio vulnificus: new insights into a deadly opportunistic pathogen", Environ Microbiol, 20, pp.423~430 (2020)[DOI]
- Balows A, "Manual of Clinical Microbiology", pp.384~395 (1991), ASM Press
- 조의동, "곰소만 및 변산 해역 해수에 병원성 비브리오균(Vibrios spp.)의 분포", 한국수산과학회지, 52(6), pp.625~630 (2019), 한국수산과학회[DOI]
- 고연자, "인천 연안지역의 해양환경요인과 병원성 비브리오균의 분포와의 관련성", 한국식품영양학회지, 26(3), pp.414~420 (2013), 한국식품영양학회[DOI]
- 한아름, "경기인천 연안에서 분리된 장염비브리오균의 항생제 내성 및 플라스미드 보유 현황", 미생물학회지, 48(1), pp.22~28 (2012), 한국미생물학회
- Honda T, "The pathogenicity of Vibrio parahaemolyticus and the role of the thermostable direct haemolysin and related haemolysins", Rev Med Microbiol, 4, pp.106~113 (1993)
- 정영일, "2016년도 국내 해양환경내 병원성 비브리오균의 분포 및 해양환경인자간의 상관성 분석", 한국환경보건학회지, 44(2), pp.133~142 (2018), 한국환경보건학회
- "Infectious Disease Portal" (2022)[URL]
- Kim HJ, "Multiplex PCR for detection of the Vibrio genus and five pathogenic Vibrio species with primer sets designed using comparative genomics", BMC Microbiol, 15, pp.239~ (2015)[DOI]
- 김수경, "양식 굴(Crassostrea gigas)에서 분리된 장염비브리오균의독소 유전자 보유 및 항균제 감수성", 한국수산과학회지, 49(2), pp.116~123 (2016), 한국수산과학회
- Lippi D, "Cholera", Microbiol Spectrum, 4, pp.1~6 (2016)[DOI]
- Makino K, "Genome sequence of Vibrio parahaemolyticus: A pathogenic mechanism distinct from that of V. cholerae", Lancet, 361, pp.743~749 (2003)[DOI]
- "Food Poisoning Statistics" (2022)[URL]
- The relationship of pathogenic Vibrio spp. with marine environmental factors, Korea, 2013-2015, Public Health Weekly Report, "Na HY", Korea Centers for Disease Control Prevention, 154~158 (2016)
- Nandi B, "Rapid method for species-specific identification of Vibrio cholerae using primers targeted to the gene of outer membrane protein OmpW", J Clin Microbiol, 38, pp.4145~4151 (2000)[DOI]
- No AR, "Rapid detection of Vibrio parahaemolyticus by PCR targeted to the histone-like nucleoid structure (H-NS) gene and its genetic characterization", Lett Appl Microbiol, 53, pp.127~133 (2011)[DOI]
- 오희경, "가덕도 연안 해수에서 분리된 Vibrio vulnificus의 검출 특성 및 항생제 내성", 한국수산과학회지, 54(6), pp.912~917 (2021), 한국수산과학회[DOI]
- 박큰바위, "2013-2016년 경남 연안 해수 및 패류에서 병원성 비브리오균의 계절적 및 지역적 변동", 한국수산과학회지, 52(1), pp.27~34 (2019), 한국수산과학회[DOI]
- Thompson FL, "The Biology of Vibrios" (2006), ASM Press
- "Bacteriological Analytical Manual" (2019)[URL]
- 유홍식, "한국 연안산 패류 중 Vibrio parahaemolyticus의 분포 및 항생제내성 특성 비교", 한국수산과학회지, 47(5), pp.508~515 (2014), 한국수산과학회
- Zhang L, "Virulence determinants for Vibrio parahaemolyticus infection", Curr Opin Microbiol, 16, pp.70~77 (2013)[DOI]