가덕도 연안 해수에서 분리된 Vibrio vulnificus의 검출 특성 및 항생제 내성
Detection Characteristics and Antimicrobial Resistance of Vibrio vulnificus Isolated from Sea Water Along the Gadeok Island Coast
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We investigated the distribution, molecular characteristics, and antimicrobial resistance of Vibrio vulnificus isolated from seawater at Gadeok Island, the Republic of Korea between June to October. Interestingly no isolates were detected between December to February. The detection rate of V. vulnificus was high (80-100%) from July to September 2019 and from June to September 2020. This coincided with the relatively low salinity of the seawater, which ranged from 7.8-29.9 practical salinity units for that period. Additionally, V. vulnificus had a high detection rate at sampling stations near the Nakdong river. The detection rates of virulence genes, such as vvhA, viuB, and vcgC, among the isolates were 97.1%, 44.1%, and 57.4% in 2019 and 100%, 43.0%, and 50.0% in 2020, respectively. Notably, viuB and vcgC were detected in V. vulnificus isolated between June to October when water temperature was above 20°C. The antimicrobial susceptibility analysis of 80 isolates revealed that most of the strains were susceptible to most antimicrobial agents. However, some isolates showed intermediate resistance to cefepime (18.8%), cefoxitin (58.8%), and erythromycin (22.5%). Of note, 3.8% of the tested strains were resistant to cefoxitin. The minimum inhibitory concentration of highly cefoxitin-resistant strains was determined to be less than 32 μg/mL.
Abstract
We investigated the distribution, molecular characteristics, and antimicrobial resistance of Vibrio vulnificus isolated from seawater at Gadeok Island, the Republic of Korea between June to October. Interestingly no isolates were detected between December to February. The detection rate of V. vulnificus was high (80-100%) from July to September 2019 and from June to September 2020. This coincided with the relatively low salinity of the seawater, which ranged from 7.8-29.9 practical salinity units for that period. Additionally, V. vulnificus had a high detection rate at sampling stations near the Nakdong river. The detection rates of virulence genes, such as vvhA, viuB, and vcgC, among the isolates were 97.1%, 44.1%, and 57.4% in 2019 and 100%, 43.0%, and 50.0% in 2020, respectively. Notably, viuB and vcgC were detected in V. vulnificus isolated between June to October when water temperature was above 20°C. The antimicrobial susceptibility analysis of 80 isolates revealed that most of the strains were susceptible to most antimicrobial agents. However, some isolates showed intermediate resistance to cefepime (18.8%), cefoxitin (58.8%), and erythromycin (22.5%). Of note, 3.8% of the tested strains were resistant to cefoxitin. The minimum inhibitory concentration of highly cefoxitin-resistant strains was determined to be less than 32 μg/mL.
참고문헌 (32)
- Acar JF, "Antibiotics in laboratory medicine", pp.17~52 (1991), Williams & Wilkins
- Craig Baker-Austin, "Emerging Vibrio risk at high latitudes in response to ocean warming", Nature Climate Change, 3(1), pp.73~77 (2013), Springer Science and Business Media LLC[DOI]
- Sedat Çam, "Quantitative PCR enumeration of vcgC and 16S rRNA type A and B genes as virulence indicators for environmental and clinical strains of Vibrio vulnificus in Galveston Bay oysters", Canadian Journal of Microbiology, 65(8), pp.613~621 (2019), Canadian Science Publishing[DOI]
- 조의동, "곰소만 및 변산 해역 해수에 병원성 비브리오균(Vibrios spp.)의 분포", 한국수산과학회지, 52(6), pp.625~630 (2019), 한국수산과학회[DOI]
- Han Young Chung, "Complete genome sequence of Vibrio vulnificus FORC_017 isolated from a patient with a hemorrhagic rash after consuming raw dotted gizzard shad", Gut Pathogens, 8(1), pp.1~6 (2016), Springer Science and Business Media LLC[DOI]
- CLSI (Clinical and Laboratory Standards Institute), "Methods for antimicrobial dilution and disk susceptibility testing of infrequently isolated or fastidious bacteria. 3rd ed. CLSI guideline M45", 56~58 (2015)
- CLSI (Clinical and Laboratory Standards Institute), "Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. 11th ed. CLSI standard M07", 27~31 (2018)
- CLSI (Clinical and Laboratory Standards Institute), "Performance standards for antimicrobial susceptibility testing. 28th ed. CLSI supplement M100", 60~ (2018)
- Sara Elmahdi, "Antibiotic resistance of Vibrio parahaemolyticus and Vibrio vulnificus in various countries: A review", Food Microbiology, 57, pp.128~134 (2016), Elsevier BV[DOI]
- Feifei Han, "Characterization of Clinical and Environmental Types ofVibrio vulnificusIsolates from Louisiana Oysters", Foodborne Pathogens and Disease, 6(10), pp.1251~1258 (2009), Mary Ann Liebert Inc[DOI]
- W. G. Hlady, "The Epidemiology of Vibrio Infections in Florida, 1981-1993", Journal of Infectious Diseases, 173(5), pp.1176~1183 (1996), Oxford University Press (OUP)[DOI]
- Hwang KW, "Distribution and antibiotic resistance of Vibrio vulnificus isolated in Incheon Coastal Area", Korean J Sanitation, 22, pp.55~63 (2007)
- Monitoring of antibiotics to residue in water environment of Busan, "Jeong JE", Busan Metropolitan City Institute of Health & Environment, 110~122, pp.The Annual Rpoert of Busan Metropolitan City Institute of Health & Environment 24 (2014)
- "Reported cases of Vibrio vulnificus sepsis by year and month. Infectious disease surveillance yearbook" (2019)[URL]
- "Epidemiological characteristics of cases with V. vulnificus infection in Korea. Public Health Weekly Report 12" (2019)[URL]
- "Infectious disease portal. In Vibrio vulnificus sepsis" (2021)[URL]
- "One health AMR info" (2021)[URL]
- Klaus Kümmerer, "Antibiotics in the aquatic environment – A review – Part I", Chemosphere, 75(4), pp.417~434 (2009), Elsevier BV[DOI]
- Jaime Martinez-Urtaza, "Climate anomalies and the increasing risk of Vibrio parahaemolyticus and Vibrio vulnificus illnesses", Food Research International, 43(7), pp.1780~1790 (2010), Elsevier BV[DOI]
- M. L. Motes, "Influence of Water Temperature and Salinity on Vibrio vulnificus in Northern Gulf and Atlantic Coast Oysters ( Crassostrea virginica )", Applied and Environmental Microbiology, 64(4), pp.1459~1465 (1998), American Society for Microbiology[DOI]
- 오희경, "가덕도 연안 해수에서 Vibrio vulnificus의 분포 및 분리균주의 병원성 유전자 특성", 한국수산과학회지, 53(5), pp.688~693 (2020), 한국수산과학회[DOI]
- 오향균, "도시하수 및 그 주변 하천 환경 중 항생제 내성 세균 노출 특성", 대한환경공학회지, 31(3), pp.232~239 (2009), 대한환경공학회
- Anthony I Okoh, "Antibiotic susceptibility profiles of some Vibrio strains isolated from wastewater final effluents in a rural community of the Eastern Cape Province of South Africa", BMC Microbiology, 10(1), pp.143~ (2010), Springer Science and Business Media LLC[DOI]
- J. D. OLIVER, "Wound infections caused by Vibrio vulnificus and other marine bacteria", Epidemiology and Infection, 133(3), pp.383~391 (2005), Cambridge University Press (CUP)[DOI]
- 박큰바위, "2013-2016년 경남 연안 해수 및 패류에서 병원성 비브리오균의 계절적 및 지역적 변동", 한국수산과학회지, 52(1), pp.27~34 (2019), 한국수산과학회[DOI]
- 성진욱, "낙동강에서 엔테로코커스의 분포특성 및 항생제별 내성률", 생태와 환경, 46(3), pp.360~366 (2013), 한국수생태학회
- R. L. Shapiro, "The Role of Gulf Coast Oysters Harvested in Warmer Months inVibrio vulnificusInfections in the United States, 1988–1996", The Journal of Infectious Diseases, 178(3), pp.752~759 (1998), Oxford University Press (OUP)[DOI]
- 신성희, "Multiple High-affinity Iron-uptake Systems of Vibrio vulnificus", Journal of Bacteriology and Virology, 43(3), pp.168~176 (2013), 대한미생물학회[DOI]
- Mark S. Strom, "Epidemiology and pathogenesis of Vibrio vulnificus", Microbes and Infection, 2(2), pp.177~188 (2000), Elsevier BV[DOI]
- "BAM chapter 9: Vibrio" (2018)[URL]
- Yang JH, "Distribution and antimicrobial susceptibility of Vibrio species associated with zooplankton in coastal area of Korea", Mar Pollut Bull, 125, pp.39~44 (2017)[DOI]
- 윤연희, "어류, 수족관수 및 환자에서 분리된 Vibrio vulnificus의 독소유전자 분포 및 항생제 내성", 한국식품위생안전성학회지, 35(1), pp.6~12 (2020), 한국식품위생안전성학회[DOI]