본문 바로가기
KERD
논문KCI 등재

Protective effects of Hizikia fusiforme and Chlorella sp. extracts against lead acetateinduced hepatotoxicity in rats

2

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류수산학

초록

In the present study, the protective effects of Hizikia fusiforme and Chlorella sp. extracts on lead acetate-induced hepatotoxicity were investigated. Hepatic damage was induced in rats by intraperitoneal (i.p.) injection of lead acetate and the protective effects of H. fusiforme (HZK) and Chlorella sp. (CHL) extracts on lead acetate-induced hepatic damage in rat liver were examined. The results revealed significantly increased glutamic oxaloacetate and glutamic pyruvic transaminase levels in the group treated with lead acetate only (Pb group); oral administration of HZK and CHL extracts tended to decrease the enzyme levels similar to those observed in the control group. Regarding antioxidant enzymes, superoxide dismutase activity was increased in the Pb group and decreased in a concentration-dependent manner in the HZK- and CHL-treated groups. Glutathione levels were increased in a concentration-dependent manner in the HZK- and CHL-treated groups. There was no significant difference in catalase activity. Western blot analysis showed inflammation-related protein expression in mitogen-activated protein kinase and Nrf2 pathways was affected in the HZK- and CHL-treated groups. Therefore, HZK and CHL extracts exerted antioxidant and anti-inflammatory effects against lead acetate-induced hepatotoxicity. Development of functional health foods containing HZK and CHL extracts, which have hepatoprotective effects against inhaled lead acetate, should be considered.

Abstract

In the present study, the protective effects of Hizikia fusiforme and Chlorella sp. extracts on lead acetate-induced hepatotoxicity were investigated. Hepatic damage was induced in rats by intraperitoneal (i.p.) injection of lead acetate and the protective effects of H. fusiforme (HZK) and Chlorella sp. (CHL) extracts on lead acetate-induced hepatic damage in rat liver were examined. The results revealed significantly increased glutamic oxaloacetate and glutamic pyruvic transaminase levels in the group treated with lead acetate only (Pb group); oral administration of HZK and CHL extracts tended to decrease the enzyme levels similar to those observed in the control group. Regarding antioxidant enzymes, superoxide dismutase activity was increased in the Pb group and decreased in a concentration-dependent manner in the HZK- and CHL-treated groups. Glutathione levels were increased in a concentration-dependent manner in the HZK- and CHL-treated groups. There was no significant difference in catalase activity. Western blot analysis showed inflammation-related protein expression in mitogen-activated protein kinase and Nrf2 pathways was affected in the HZK- and CHL-treated groups. Therefore, HZK and CHL extracts exerted antioxidant and anti-inflammatory effects against lead acetate-induced hepatotoxicity. Development of functional health foods containing HZK and CHL extracts, which have hepatoprotective effects against inhaled lead acetate, should be considered.

참고문헌 (34)

  1. Aktan F, "iNOS-mediated nitric oxide production and its regulation", Life Sci, 75, pp.639~653 (2004)
  2. Aránzazu Bocanegra, "Characteristics and Nutritional and Cardiovascular-Health Properties of Seaweeds", Journal of Medicinal Food, 12(2), pp.236~258 (2009), 한국식품영양과학회
  3. Bozena K, "MAPK signalling pathways as molecular targets for anti-inflammatory therapy from molecular mechanisms to therapeutic benefits", Biochim Biophys Acta, 1754, pp.253~262 (2005)
  4. Cargnello M, "Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases", Microbiol Mol Biol Rev, 75, pp.50~83 (2011)
  5. Carla SC, "Data on the effects of losartan on protein expression vascular reactivity and antioxidant capacity in the aorta of ethanol-treated rats", Data Brief, 11, pp.111~116 (2017)
  6. Dao VT, "Catalase activity prevents exercise-induced up-regulation of vasoprotective proteins in venous tissue", J Cell Mol Med, 15, pp.2326~2334 (2001)
  7. Didonato JA, "NF-κB and the link between inflammation and cancer", Immunol Rev, 246, pp.379~400 (2012)
  8. Donaldson K, "Potential mechanisms of adverse pulmonary and cardiovascular effects of particulate air pollution(PM10)", Int J Hyg Environ Health, 203, pp.411~415 (2001)
  9. Fukai T, "Superoxide dismutases : role in redox signaling, vascular function, and diseases", Antioxid Redox Signal, 15, pp.1583~1606 (2011)
  10. Hidalgo S, "Benefits of oral and topical administration of ROQUETTE Chlorella sp. on skin inflammation and wound healing in mice", Antiinflamm Antiallergy Agents Med Chem, 13, pp.93~102 (2014)
  11. Hsiao W, "Cytotoxicity of PM2.5 and PM2.5-10 ambient air pollutants assessed by the MTT and the comet assays", Mutat Res, 471, pp.45~55 (2000)
  12. Hu X, "Lead contamination and transfer in urban environmental compartments analyzed by lead levels and isotopic compositions", Environ Pollut, 187, pp.42~48 (2014)
  13. Johny CP, "Protective effects of Spirulina maxima on hyperlipidemia and oxidative-stress induced by lead acetate in the liver and kidney", Lipids Health Dis, 9, pp.35~ (2010)
  14. Justo GZ, "Effects of the green algae Chlorella vulgaris on the response of the host hematopoietic system to intraperitoneal ehrlich ascites tumor transplantation in mice", Immunopharmacol Immunotoxicol, 23, pp.119~132 (2001)
  15. 김향숙, "톳 추출물의 경구투여가 흰쥐의 항산화효소 활성과 비타민 E 농도에 미치는 영향", 한국식품영양과학회지, 40(11), pp.1556~1561 (2011), 한국식품영양과학회
  16. Kim HS, "Effects of the feeding Hizikia fusiforme(Harvey)Okamura on lipid composition of serum in dietary hyperlipidemic rats", J Korean Soc Food Sci Nutr, 27, pp.718~723 (1998)
  17. Kim YC, "The effect of UVB irradiation on the generating and scavenging enzymes of oxygen free radical in mouse skin", J NERI, 10, pp.49~55 (2005)
  18. 이영애, "다시마 추출물이 난소를 절제한 흰 쥐의 혈 중 지질 함량에 미치는 영향", 생명과학회지, 18(2), pp.249~254 (2008), 한국생명과학회
  19. Li P, "Remote limb ischemic postconditioning protects mouse brain against cerebral ischemia", reperfusion injury via upregulating expression of Nrf2, HO-1 and NQO-1 in mice, 2015, pp.17, 1~8
  20. Li XD, "Effects of high intensity exhaustive exercise on SOD, MDA, and NO levels in rats with knee osteoarthritis", Genet Mol Res, 14, pp.12367~12376 (2015)
  21. Liu CM, "Proanthocyanidins improves lead-induced cognitive impairments by blocking endoplasmic reticulum stress and nuclear factor-κB-mediated inflammatory pathways in rats", Food Chem Toxicol, 72, pp.295~302 (2014)
  22. Meo SA, "Effect of environmental air pollution on cardiovascular diseases", Eur Rev Med Pharmacol Sci, 19, pp.4890~4897 (2015)
  23. Moon TC, "Regulation of cyclooxygenase-2 and endogenous cytokine expression by bacterial lipopolysaccharide that acts in synergy with c-kit ligand and Fc epsilon receptor I crosslinking in cultured mast cells", Cell Immunol, 185, pp.146~152 (1998)
  24. Murakami S, "Roles of Nrf2 in cell proliferation and differentiation", Free Radic Biol Med, 88, pp.168~178 (2015)
  25. Newairy AS, "Protective role of flax lignans against lead acetate induced oxidative damage and hyperlipidemia in rats", Food Chem Toxicol, 47, pp.813~818 (2009)
  26. Obata K, "MAPK activation in nociceptive neurons and pain hypersensitivity", Life Sci, 74, pp.2643~2653 (2004)
  27. Pairat K, "Cadmium(II)removal from aqueous solutions by pre-treated biomass of marine alga Padina sp", Environ Pollut, 112, pp.209~213 (2001)
  28. Park EM, "Low GPX activities in RBC predict susceptibility of lung cancer patients to radiation pneumonitis", Free Radic Biol Med, 42, pp.280~287 (2007)
  29. Singh A, "Perinatal influence of Chlorella vulgris(E-25)on hepatic drug metabolizing enzymes and lipid peroxidation", Anticancer Res, 18, pp.1509~1514 (1998)
  30. Wang C, "The role of catalase in the immune response to oxidative stress and pathogen challenge in the clam Meretrix meretrix", Fish Shellfish Immunol, 34, pp.91~99 (2013)
  31. Widmann C, "Mitogen-activated protein kinase : conservation of a three-kinase module from yeast to human", Physiol, 79, pp.143~180 (1999)
  32. Wua M, "Evaluation of antioxidant activities of watersoluble polysaccharides from brown alga Hizikia fusiformis", Int J Biol Macromol, 56, pp.28~33 (2013)
  33. Yang J, "Protective effect of apigenin on mouse acute liver injury induced by acetaminophen is associated with increment of hepatic glutathione reductase activity", Food Funct, 4, pp.939~943 (2013)
  34. Zhou X, "Concentrations, correlations and chemical species of PM2.5", PM10 based on published data in China: potential implications for the revised particulate standard, 2016, pp.144, 518~526

관련 논문AI추천

이 간행물의 다른 논문 (6건)