Hexane fraction from the ethanolic extract of Sargassum serratifolium suppresses cell adhesion molecules via regulation of NF-κB and Nrf2 pathway in human umbilical vein endothelial cells
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Sargassum serratifolium ethanolic extract has been known for strong antioxidant and anti-inflammatory properties. We prepared hexane fraction from the ethanolic extract of S. serratifolium (HSS) to improve biological activities. In this study, we investigated the effects of HSS on the inhibition of tumor necrosis factor (TNF)-α-induced monocyte adhesion to human umbilical vein endothelial cells (HUVECs). We found that HSS suppressed the production of cell adhesion molecules such as intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 in TNF-α-induced HUVECs. Moreover, TNF-α-induced production of monocyte chemoattractant protein 1 and keratinocyte chemoattractant was inhibited by HSS treatment. HSS suppressed TNF-α-induced nuclear factor kappa B (NF-κB) activation via preventing proteolytic degradation of inhibitor κB-α. HSS induced the production of heme oxygenase 1 via translocation of Nrf2 into the nucleus in TNF-α-treated HUVECs. Overall, HSS alleviated vascular inflammation through the downregulation of NF-κB activation and the upregulation of Nrf2 activation in TNF-α-induced HUVECs. These results indicate that HSS may be used as therapeutic agents for vascular inflammatory disorders.
Abstract
Sargassum serratifolium ethanolic extract has been known for strong antioxidant and anti-inflammatory properties. We prepared hexane fraction from the ethanolic extract of S. serratifolium (HSS) to improve biological activities. In this study, we investigated the effects of HSS on the inhibition of tumor necrosis factor (TNF)-α-induced monocyte adhesion to human umbilical vein endothelial cells (HUVECs). We found that HSS suppressed the production of cell adhesion molecules such as intracellular adhesion molecule-1 and vascular cell adhesion molecule-1 in TNF-α-induced HUVECs. Moreover, TNF-α-induced production of monocyte chemoattractant protein 1 and keratinocyte chemoattractant was inhibited by HSS treatment. HSS suppressed TNF-α-induced nuclear factor kappa B (NF-κB) activation via preventing proteolytic degradation of inhibitor κB-α. HSS induced the production of heme oxygenase 1 via translocation of Nrf2 into the nucleus in TNF-α-treated HUVECs. Overall, HSS alleviated vascular inflammation through the downregulation of NF-κB activation and the upregulation of Nrf2 activation in TNF-α-induced HUVECs. These results indicate that HSS may be used as therapeutic agents for vascular inflammatory disorders.
참고문헌 (30)
- Ayer A, "Heme oxygenases in cardiovascular health and disease", Physiol Rev, 96, pp.1449~1508 (2016)
- Azam MS, "Ethanolic extract from Sargassum serratifolium attenuates hyperpigmentation through CREB", ERK signaling pathways in α-MSH-stimulated B16F10 melanoma cells, 2017, pp.29, 2089~2096
- Catalan U, "Protective effect of hydroxytyrosol and its predominant plasmatic human metabolites against endothelial dysfunction in human aortic endothelial cells", Mol Nutri Food Res, 59, pp.2523~2536 (2015)
- Catapano AL, "Vascular inflammation and low-density lipoproteins : is cholesterol the link? A lesson from the clinical trials", Br J Pharmacol, 174, pp.3973~3985 (2017)
- Chen YM, "Dual regulation of tumor necrosis factor-α-induced CCL2", monocyte chemoattractant protein-1 expression in vascular smooth muscle cells by nuclear factor-κB and activator protein-1 : modulation by type III phosphodiesterase inhibition, 2004, pp.309, 978~986
- Choi ES, "Ligustilide attenuates vascular inflammation and activates Nrf2", HO-1 induction and, NO synthesis in HUVECs, 2018, pp.38, 12~23
- De Filippo K, "Neutrophil chemokines KC and macrophage-inflammatory protein-2 are newly synthesized by tissue macrophages using distinct TLR signaling pathways", J Immunol, 180, pp.4308~4315 (2008)
- Gwon WG, "Sargachromenol protects against vascular inflammation by preventing TNF-α-induced monocyte adhesion to primary endothelial cells via inhibition of NF-κB activation", Int Immunopharmacol, 42, pp.81~89 (2017)
- Gwon WG, "Meroterpinoid-rich fraction of the ethanol extract from Sargassum serratifolium suppresses TNF-α-induced monocytes adhesion to vascular endothelium and vascular inflammation in high cholesterol-fed C57BL", 6J mice, 2018, pp.46, 384~393
- Gwon WG, "Sargaquinoic acid inhibits TNF-α-induced NF-κB signaling, thereby contributing to decreased monocyte adhesion to human umbilical vein endothelial cells(HUVECs)", J Agric Food Chem, 63, pp.9053~9061 (2015)
- Huang W, "Oridonin inhibits vascular inflammation by blocking NF-κB and MAPK activation", Eur J Pharmacol, 826, pp.133~139 (2018)
- Huo Y, "The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium", J Clin Invest, 108, pp.1307~1314 (2001)
- Ishikawa K, "Heme oxygenase-1 inhibits atherosclerotic lesion formation in ldl-receptor knockout mice", Circ Res, 88, pp.506~512 (2001)
- Joung EJ, "Anti-inflammatory action of the ethanolic extract from Sargassum serratifolium on lipopolysaccharidestimulated mouse peritoneal macrophages and identification of active components", J Appl Phycol, 29, pp.563~573 (2017)
- Joung EJ, "Sargaquinoic acid attenuates inflammatory responses by regulating NF-κB and Nrf2 pathways in lipopolysaccharide-stimulated RAW 264.7 cells", Int Immunopharmacol, 29, pp.693~700 (2015)
- Kato H, "Heme oxygenase-1 overexpression protects rat livers from ischemia", reperfusion injury with extended cold preservation, 2001, pp.1, 121~128
- Kawamura K, "Bilirubin from heme oxygenase-1 attenuates vascular endothelial activation and dysfunction", Arterioscler Thromb Vasc Biol, 25, pp.155~160 (2005)
- Kim JH, "The non-provitamin A carotenoid, lutein, inhibits NF-κB-dependent gene expression through redox-based regulation of the phosphatidylinositol 3-kinase", PTEN, Free Rad Biol Med, pp.2008, 45[DOI]
- Kleinbongard P, "TNFα in atherosclerosis, myocardial ischemia", reperfusion and heart failure, 2010, pp.127, 295~314
- Kwon M, "Meroterpenoid-rich fraction of an ethanolic extract from Sargassum serratifolium alleviates obesity and nonalcoholic fatty liver disease in high fat-fed C57BL", 6J mice, 2018, pp.47, 288~298
- Lim S, "Evaluation of antioxidant activities of various solvent extract from Sargassum serratifolium and its major antioxidant components", Food Chem, 278, pp.178~184 (2019)
- Lim S, "Meroterpenoid-rich fraction of the ethanolic extract from Sargassum serratifolium suppressed oxidative stress induced by tert-butyl hydroperoxide in HepG2 cells", Mar Drugs, 16, pp.374~ (2018)
- Liu L, "Towards a better understanding of medicinal uses of the brown seaweed Sargassum in traditional Chinese medicine : a phytochemical and pharmacological review", J Ethnopharmacol, 142, pp.591~619 (2012)
- Madge LA, "TNF signaling in vascular endothelial cells", Exp Mol Pathol, 70, pp.317~325 (2001)
- Nallasamy P, "Sulforaphane reduces vascular inflammation in mice and prevents TNF-α-induced monocyte adhesion to primary endothelial cells through interfering with the NF-κB pathway", J Nutr Biochem, 25, pp.824~833 (2014)
- Namiki M, "Local overexpression of monocyte chemoattractant protein-1 at vessel wall induces infiltration of macrophages and formation of atherosclerotic lesion : synergism with hypercholesterolemia", Arterioscler Thromb Vas Biol, 22, pp.115~120 (2002)
- 오선지, "Anti-Inflammatory Effect of Ethanolic Extract of Sargassum serratifolium in Lipopolysaccharide-Stimulated BV2 Microglial Cells", Journal of Medicinal Food, 19(11), pp.1023~1031 (2016), 한국식품영양과학회[DOI]
- Packard RR, "Inflammation in atherosclerosis : from vascular biology to biomarker discovery and risk prediction", Clin Chem, 54, pp.24~38 (2008)
- Rao RM, "Endothelial-dependent mechanisms of leukocyte recruitment to the vascular wall", Circ Res, 101, pp.234~247 (2007)
- Zhang H, "Role of TNF-α in vascular dysfunction", Clin Sci, 116, pp.219~230 (2009)