Extracts of abalone intestine regulates fat metabolism in 3T3-L1 adipocytes and high fat dietinduced zebrafish larvae
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Obesity is a disease involving mechanisms of fat accumulation, low-grade inflammatory cytokine release, and mitochondrial dysfunction. The aim of the current study was to investigate the effects of abalone intestine extract on fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae. The total phenol content was highest in subcritical water extract at 210℃ (SW210) among hot water, ethanol, and subcritical water extracts of abalone intestine. In addition, SW210 of male abalone intestine (MASW210) most effectively controlled the lipid accumulation and expression of adipogenic or lipogenic regulators (PPAR-γ, C/EBPα, SREBP-1c, and FAS) in 3T3-L1 adipocytes. Likewise, in zebrafish larvae fed high fat, MASW210 significantly suppressed body weight, glucose levels, and lipid accumulation. The mRNA expression related to adipogenesis (PPAR-γ and C/EBPα), lipogenesis (SREBP-1c and FAS), inflammatory cytokines (TNF-α and IL-6), energy m/;.etabolism (AMPK, lepr, SIRT1, and adiponectin), and mitochondrial biogenesis (PGC-1α and CPT-1) were significantly regulated by treatment with MASW210. These results suggest that abalone intestine extract such as MASW210, are useful biomaterials for improving obesity and metabolic diseases.
Abstract
Obesity is a disease involving mechanisms of fat accumulation, low-grade inflammatory cytokine release, and mitochondrial dysfunction. The aim of the current study was to investigate the effects of abalone intestine extract on fat metabolism in 3T3-L1 adipocytes and high fat diet-induced zebrafish larvae. The total phenol content was highest in subcritical water extract at 210℃ (SW210) among hot water, ethanol, and subcritical water extracts of abalone intestine. In addition, SW210 of male abalone intestine (MASW210) most effectively controlled the lipid accumulation and expression of adipogenic or lipogenic regulators (PPAR-γ, C/EBPα, SREBP-1c, and FAS) in 3T3-L1 adipocytes. Likewise, in zebrafish larvae fed high fat, MASW210 significantly suppressed body weight, glucose levels, and lipid accumulation. The mRNA expression related to adipogenesis (PPAR-γ and C/EBPα), lipogenesis (SREBP-1c and FAS), inflammatory cytokines (TNF-α and IL-6), energy m/;.etabolism (AMPK, lepr, SIRT1, and adiponectin), and mitochondrial biogenesis (PGC-1α and CPT-1) were significantly regulated by treatment with MASW210. These results suggest that abalone intestine extract such as MASW210, are useful biomaterials for improving obesity and metabolic diseases.
참고문헌 (50)
- Achari AE, "Adiponectin, a therapeutic target for obesity, diabetes, and endothelial dysfunction", Int J Mol Sci, 18, pp.1321~ (2017)
- Alkhatib A, "Functional foods and lifestyle approaches for diabetes prevention and management", Nutrients, 9, pp.1310~ (2017)
- Bullon N, "The role of aquafeeds in abalone nutrition and health : a comprehensive review", J World Aquac Soc, 54, pp.7~31 (2022)
- Cheng M, "Overview of structure, function and integrated utilization of marine shell", Sci Total Environ, 870, pp.161950~ (2023)
- Cheng Y, "Subcritical water extraction of natural products", Molecules, 26, pp.4004~ (2021)
- Cook PA, "The international abalone market", Dev Aquac Fisher Sci, 42, pp.373~382 (2023)
- De Zoysa M., "Marine nutraceuticals: prospects and perspectives", pp.57~70 (2013), CRC Press
- Den Broeder MJ, "Altered adipogenesis in zebrafish larvae following high fat diet and chemical exposure is visualised by stimulated Raman scattering microscopy", Int J Mol Sci, 18, pp.894~ (2017)
- Du J, "An optimized method for Oil Red O staining with the salicylic acid ethanol solution", Adipocyte, 12, pp.2179334~ (2023)
- Fontecha-Barriuso M, "The role of PGC-1α and mitochondrial biogenesis in kidney diseases", Biomolecules, 10, pp.347~ (2020)
- Göransson O, "Metabolic control by AMPK in white adipose tissue", Trends Endocrinol Metab, 34(11), pp.704~717 (2023)
- Grahame Hardie D, "AMPK : a nutrient and energy sensor that maintains energy homeostasis", Nat Rev Mol Cell Biol, 13, pp.251~262 (2012)
- Green M, "Microbial medicine : prebiotic and probiotic functional foods to target obesity and metabolic syndrome", Int J Mol Sci, 21, pp.2890~ (2020)
- Jakab J, "Adipogenesis as a potential anti-obesity target : a review of pharmacological treatment and natural products", Diabetes Metab Syndr Obes, 14, pp.67~83 (2021)
- Kang NH, "Trans-anethole ameliorates obesity via induction of browning in white adipocytes and activation of brown adipocytes", Biochimie, 151, pp.1~13 (2018)
- Khan BM, "Marine mollusks : food with benefits", Compr Rev Food Sci Food Saf, 18, pp.548~564 (2019)
- Kojta I, "Obesity, bioactive lipids, and adipose tissue inflammation in insulin resistance", Nutrients, 12, pp.1305~ (2020)
- Kwon J, "Comprehensive amelioration of high-fat diet-induced metabolic dysfunctions through activation of the PGC-1α pathway by probiotics treatment in mice", PLOS ONE, 15, pp.e0228932~ (2020)
- Lee H, "Energy-stress-mediated AMPK activation inhibits ferroptosis", Nat Cell Biol, 22, pp.225~234 (2020)
- Lee HB, "Analysis of the physiological responses of Pacific abalone(Haliotis discus hannai)to various stressors to identify a suitable stress indicator", J World Aquac Soc, 54, pp.1407~1426 (2023)
- Li H, "Oxidative stress : the nexus of obesity and cognitive dysfunction in diabetes", Front Endocrinol, 14, pp.1134025~ (2023)
- Lin D, "An overview of plant phenolic compounds and their importance in human nutrition and management of type 2 diabetes", Molecules, 21, pp.1374~ (2016)
- Long YC, "AMP-activated protein kinase signaling in metabolic regulation", J Clin Invest, 116, pp.1776~1783 (2006)
- Mariani S, "Blood SIRT1 shows a coherent association with leptin and adiponectin in relation to the degree and distribution of adiposity : a study in obesity, normal weight and anorexia nervosa", Nutrients, 12, pp.3506~ (2020)
- Nguyen MHT, "Beneficial effect of abalone intestine gastro-intestinal digests on osteoblastic MG-63 cell differentiation", J Aquat Food Prod Technol, 23, pp.436~446 (2014)
- Nguyen PH, "Protective effects of in vitro gastrointestinal digests of abalone (Haliotis discus hannai) intestines against oxidative stress in RAW264.7 macrophage cells", Fish Aquat Sci, 13, pp.216~223 (2010)
- Obradovic M, "Leptin and obesity : role and clinical implication", Front Endocrinol, 12, pp.585887~ (2021)
- Park CJ, "Abalone aquaculture in Korea", J Shellfish Res, 32, pp.17~19 (2013)
- Qian ZJ, "The antioxidant and anti-inflammatory effects of abalone intestine digest, Haliotis discus hannai in RAW 264.7 macrophages", Biotechnol Bioprocess Eng, 17, pp.475~484 (2012)
- Rahman MM, "Role of phenolic compounds in human disease : current knowledge and future prospects", Molecules, 27, pp.233~ (2021)
- Romulo A, "Extraction of phenolic compounds using subcritical hot water extraction : a review", IOP Conf Ser Earth Environ Sci, 426, pp.012173~ (2020)
- Roy B, "Tissue-specific role and associated downstream signaling pathways of adiponectin", Cell Biosci, 11, pp.77~ (2021)
- Ruiz-Ojeda FJ, "Obesity: oxidative stress and dietary antioxidants", pp.1~15 (2018), Academic Press
- Rumbaoa RGO, "Phenolic content and antioxidant capacity of Philippine sweet potato(Ipomoea batatas)varieties", Food Chem, 113, pp.1133~1138 (2009)
- Salminen A, "AMP-activated protein kinase inhibits NF-κB signaling and inflammation : impact on healthspan and lifespan", J Mol Med, 89, pp.667~676 (2011)
- Shin TS, "Anti-inflammatory effects of abalone (Haliotis discus hannai) viscera via inhibition of ROS production in LPS stimulated RAW 264.7 cells", Microbiol Biotechnol Lett, 50, pp.22~30 (2022)
- Singh A, "StatPearls" (2023), StatPearls
- Thakuri LS, "Protective effect of Capsosiphon fulvescens on oxidative stress-stimulated neurodegenerative dysfunction of PC12 cells and zebrafish larva models", Fish Aquat Sci, 26, pp.24~34 (2023)
- Thakuri LS, "Subcritical water extraction of Gracilaria chorda abbreviates lipid accumulation and obesity-induced inflammation", Algae, 38, pp.81~92 (2023)
- Thakuri LS, "Gracilaria chorda subcritical-water extracts as ameliorant of insulin resistance induced by high-glucose in zebrafish and dexamethasone in L6 myotubes", J Tradit Complement Med, 14, pp.82~90 (2023)
- Troell M, "Abalone farming in South Africa : an overview with perspectives on kelp resources, abalone feed, potential for on-farm seaweed production and socio-economic importance", Aquaculture, 257, pp.266~281 (2006)
- Wang L, "Effect of dietary protein, vitamin E, and vitamin C levels on the growth and gonad development of hybrid male abalone (Haliotis fulgens ♂ × H. discus hannai ♀) by the orthogonal array design", Aquac Int, pp.1~23 (2023)
- Wang S, "Neohesperidin enhances PGC-1α-mediated mitochondrial biogenesis and alleviates hepatic steatosis in high fat diet fed mice", Nutr Diabetes, 10, pp.27~ (2020)
- Wang Q, "The new role of AMP-activated protein kinase in regulating fat metabolism and energy expenditure in adipose tissue", Biomolecules, 11, pp.1757~ (2021)
- Wen X, "Signaling pathways in obesity : mechanisms and therapeutic interventions", Signal Transduct Target Ther, 7, pp.298~ (2022)
- Yen GC, "The potential role of phenolic compounds on modulating gut microbiota in obesity", J Food Drug Anal, 28, pp.195~205 (2020)
- "Endocrine changes in obesity" (2022)[URL]
- Yu W, "Exploring the intestinal microbiota and metabolome profiles associated with feed efficiency in Pacific abalone(Haliotis discus hannai)", Front Microbiol, 13, pp.852460~ (2022)
- Zhang L, "Association of polymorphisms in LEPR with type 2 diabetes and related metabolic traits in a Chinese population", Lipids Health Dis, 17, pp.2~ (2018)
- Zheng P, "Antioxidant activities of hydrolysates from abalone viscera using subcritical water-assisted enzymatic hydrolysis", Food Bioprocess Technol, 12, pp.910~918 (2019)
