Evaluation of reparative dentin formation of ProRoot MTA, Biodentine and BioAggregate using micro-CT and immunohistochemistry
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Objectives: The purpose of this study was to assess the ability of two new calcium silicate–based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA). Materials and Methods: Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP). Results: On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP. Conclusions: Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.
Abstract
Objectives: The purpose of this study was to assess the ability of two new calcium silicate–based pulp-capping materials (Biodentine and BioAggregate) to induce healing in a rat pulp injury model and to compare them with mineral trioxide aggregate (MTA). Materials and Methods: Eighteen rats were anesthetized, cavities were prepared and the pulp was capped with either of ProRoot MTA, Biodentine, or BioAggregate. The specimens were scanned using a high-resolution micro-computed tomography (micro-CT) system and were prepared and evaluated histologically and immunohistochemically using dentin sialoprotein (DSP). Results: On micro-CT analysis, the ProRoot MTA and Biodentine groups showed significantly thicker hard tissue formation (p < 0.05). On H&E staining, ProRoot MTA showed complete dentin bridge formation with normal pulpal histology. In the Biodentine and BioAggregate groups, a thick, homogeneous hard tissue barrier was observed. The ProRoot MTA specimens showed strong immunopositive reaction for DSP. Conclusions: Our results suggest that calcium silicate-based pulp-capping materials induce favorable effects on reparative processes during vital pulp therapy and that both Biodentine and BioAggregate could be considered as alternatives to ProRoot MTA.
참고문헌 (42)
- Dominquez MS, "Histological and scanning electron microscopy assessment of various vital pulp-therapy materials", J Endod, 29, pp.324~333 (2003)
- Torabinejad M, "Clinical applications of mineral trioxide aggregate", J Endod, 25, pp.197~205 (1999)
- de Souza V, "Treatment of the inflamed dental pulp", Aust Dent J, 19, pp.191~196 (1974)
- Eskandar i zadeh A, "A comparative study on dental pulp response to calcium hydroxide, white and grey mineral trioxide aggregate as pulp capping agents", J Conserv Dent, 14, pp.351~355 (2011)
- Faraco IM Jr, "Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement", Dent Traumatol, 17, pp.163~166 (2001)
- Accorinte ML, "Response of human dental pulp capped with MTA and calcium hydroxide powder", Oper Dent, 33, pp.488~495 (2008)
- Zarrabi MH, "Histologic assessment of human pulp response to capping with mineral trioxide aggregate and a novel endodontic cement", J Endod, 36, pp.1778~1781 (2010)
- Torabinejad M, "Tissue reaction to implanted super-EBA and mineral trioxide aggregate in the mandible of guinea pigs: a preliminary report", J Endod, 21, pp.569~571 (1995)
- Moretton TR, "Tissue reactions after subcutaneous and intraosseous implantation of mineral trioxide aggregate and ethoxybenzoic acid cement", J Biomed Mater Res, 52, pp.528~533 (2000)
- Kettering JD, "Investigation of mutagenicity of mineral trioxide aggregate and other commonly used root-end filling materials", J Endod, 21, pp.537~542 (1995)
- Torabinejad M, "Sealing ability of a mineral trioxide aggregate when used as a root end filling material", J Endod, 19, pp.591~595 (1993)
- Lee SJ, "Sealing ability of a mineral trioxide aggregate for repair of lateral root perforations", J Endod, 19, pp.541~544 (1993)
- Schwartz RS, "Mineral trioxide aggregate: a new material for endodontics", J Am Dent Assoc, 130, pp.967~975 (1999)
- Holland R, "Reaction of rat connective tissue to implanted dentin tube filled with mineral trioxide aggregate, Portland cement or calcium hydroxide", Braz Dent J, 12, pp.3~8 (2001)
- Parirokh M, "Mineral trioxide aggregate:a comprehensive literature review-part III: clinical applications, drawbacks, and mechanism of action", J Endod, 36, pp.400~413 (2010)
- 장영은, "Cytotoxicity and physical properties of tricalcium silicate-based endodontic materials", Restorative Dentistry and Endodontics, 39(2), pp.89~94 (2014), 대한치과보존학회[DOI]
- Zanini M, "Biodentine induces immortalized murine pulp cell differentiation into odontoblast-like cells and stimulates biomineralization", J Endod, 38, pp.1220~1226 (2012)
- Koubi S, "Quantitative evaluation by glucose diffusion of microleakage in aged calcium silicate-based opensandwich restorations", Int J Dent, 2012, pp.105863~ (2012)
- Nowicka A, "Response of human dental pulp capped with biodentine and mineral trioxide aggregate", J Endod, 39, pp.743~747 (2013)
- Camilleri J, "Investigation of the hydration and bioactivity of radiopacified tricalcium silicate cement, Biodentine and MTA Angelus", Dent Mater, 29, pp.580~593 (2013)
- Lee BN, "Effects of 3 endodontic bioactive cements on osteogenic differentiation in mesenchymal stem cells", J Endod, 40, pp.1217~1222 (2014)
- Jung JY, "Effect of Biodentine and Bioaggregate on odontoblastic differentiation via mitogen-activated protein kinase pathway in human dental pulp cells", Int Endod J, 48, pp.177~184 (2015)
- Zhang H, "Dentin enhances the antibacterial effect of mineral trioxide aggregate and bioaggregate", J Endod, 35, pp.221~224 (2009)
- Hashem AA, "The effect of acidity on dislodgment resistance of mineral trioxide aggregate and bioaggregate in furcation perforations: an in vitro comparative study", J Endod, 38, pp.245~249 (2012)
- Grech L, "Investigation of the physical properties of tricalcium silicate cement-based root-end filling materials", Dent Mater, 29, pp.e20~e28 (2013)
- Batur YB, "The cytotoxic evaluation of mineral trioxide aggregate and bioaggregate in the subcutaneous connective tissue of rats", Med Oral Patol Oral Cir Bucal, 18, pp.e745~e751 (2013)
- Nielsen RB, "Microcomputed tomography: an advanced system for detailed endodontic research", J Endod, 21, pp.561~568 (1995)
- Verma P, "A Micro CT study of the mesiobuccal root canal morphology of the maxillary first molar tooth", Int Endod J, 44, pp.210~217 (2011)
- Iwamoto CE, "Clinical and histological evaluation of white ProRoot MTA in direct pulp capping", Am J Dent, 19, pp.85~90 (2006)
- Aeinehchi M, "Mineral trioxide aggregate (MTA) and calcium hydroxide as pulp‐capping agents in human teeth: a preliminary report", Int Endod J, 36, pp.225~231 (2003)
- Nair PN, "Histological, ultrastructural and quantitative investigations on the response of healthy human pulps to experimental capping with mineral trioxide aggregate: a randomized controlled trial", Int Endod J, 41, pp.128~150 (2008)
- Koh ET, "Cellular response to mineral trioxide aggregate", J Endod, 24, pp.543~547 (1998)
- Seux D, "Odontoblast-like cytodifferentiation of human dental pulp cells in vitro in the presence of a calcium hydroxide-containing cement", Arch Oral Biol, 36, pp.117~128 (1991)
- Laurent P, "Biodentine(TM) induces TGF-β1 release from human pulp cells and early dental pulp mineralization", Int Endod J, 45, pp.439~448 (2012)
- Laurent P, "Induction of specific cell responses to a Ca3SiO5-based posterior restorative material", Dent Mater, 24, pp.1486~1494 (2008)
- Tran XV, "Effect of a calcium-silicate-based restorative cement on pulp repair", J Dent Res, 91, pp.1166~1171 (2012)
- Yan P, "Effect of bioaggregate on differentiation of human periodontal ligament fibroblasts", Int Endod J, 43, pp.1116~1121 (2010)
- Yuan Z, "Effect of bioaggregate on mineral-associated gene expression in osteoblast cells", J Endod, 36, pp.1145~1148 (2010)
- Zhang S, "BioAggregate and iRoot BP Plus optimize the proliferation and mineralization ability of human dental pulp cells", Int Endod J, 46, pp.923~929 (2013)
- Saghiri MA, "Subcutaneous connective tissue reactions to various endodontic biomaterials: an animal study", J Dent Res Dent Clin Dent Prospects, 7, pp.15~21 (2013)
- Tziafras D, "Dentin regeneration in vital pulp therapy: design Kim J et al. principles", Adv Dent Res, 15, pp.96~100 (2001)
- Min KS, "Effect of mineral trioxide aggregate on dentin bridge formation and expression of dentin sialoprotein and heme oxygenase-1 in human dental pulp", J Endod, 34, pp.666~670 (2008)