Effects of the exposure site on histological pulpal responses after direct capping with 2 calcium-silicate based cements in a rat model
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Objectives: Direct pulp capping is a treatment for mechanically exposed pulp in which a biocompatible capping material is used to preserve pulpal vitality. Biocompatibility tests in animal studies have used a variety of experimental protocols, particularly with regard to the exposure site. In this study, pulp exposure on the occlusal and mesial surfaces of molar teeth was investigated in a rat model. Materials and Methods: A total of 58 maxillary first molars of Wistar rats were used. Forty molars were mechanically exposed and randomly assigned according to 3 factors: 1) the exposure site (occlusal or mesial), 2) the pulp-capping material (ProRoot White MTA or Bio-MA), and 3) 2 follow-up periods (1 day or 7 days) (n = 5 each). The pulp of 6 intact molars served as negative controls. The pulp of 12 molars was exposed without a capping material (n = 3 per exposure site for each period) and served as positive controls. Inflammatory cell infiltration and reparative dentin formation were histologically evaluated at 1 and 7 days using grading scores. Results: At 1 day, localized mild inflammation was detected in most teeth in all experimental groups. At 7 days, continuous/discontinuous calcified bridges were formed at exposure sites with no or few inflammatory cells. No significant differences in pulpal response according to the exposure site or calcium-silicate cement were observed. Conclusions: The location of the exposure site had no effect on rat pulpal healing. However, mesial exposures could be performed easily, with more consistent results. The pulpal responses were not significantly different between the 2 capping materials.
Abstract
Objectives: Direct pulp capping is a treatment for mechanically exposed pulp in which a biocompatible capping material is used to preserve pulpal vitality. Biocompatibility tests in animal studies have used a variety of experimental protocols, particularly with regard to the exposure site. In this study, pulp exposure on the occlusal and mesial surfaces of molar teeth was investigated in a rat model. Materials and Methods: A total of 58 maxillary first molars of Wistar rats were used. Forty molars were mechanically exposed and randomly assigned according to 3 factors: 1) the exposure site (occlusal or mesial), 2) the pulp-capping material (ProRoot White MTA or Bio-MA), and 3) 2 follow-up periods (1 day or 7 days) (n = 5 each). The pulp of 6 intact molars served as negative controls. The pulp of 12 molars was exposed without a capping material (n = 3 per exposure site for each period) and served as positive controls. Inflammatory cell infiltration and reparative dentin formation were histologically evaluated at 1 and 7 days using grading scores. Results: At 1 day, localized mild inflammation was detected in most teeth in all experimental groups. At 7 days, continuous/discontinuous calcified bridges were formed at exposure sites with no or few inflammatory cells. No significant differences in pulpal response according to the exposure site or calcium-silicate cement were observed. Conclusions: The location of the exposure site had no effect on rat pulpal healing. However, mesial exposures could be performed easily, with more consistent results. The pulpal responses were not significantly different between the 2 capping materials.
참고문헌 (33)
- Kakehashi S, "The effects of surgical exposures of dental pulps in germ-free and conventional laboratory rats", Oral Surg Oral Med Oral Pathol, 20, pp.340~349 (1965)
- ISO, "ISO 7405: 2008 Dentistry - Evaluation of biocompatibility of medical devices used in dentistry. Part 6: test procedures specific to dental materials", International Organization for Standardization, 19~27 (2008)
- Dammaschke T, "Rat molar teeth as a study model for direct pulp capping research in dentistry", Lab Anim, 44, pp.1~6 (2010)
- Decup F, "Bone sialoprotein-induced reparative dentinogenesis in the pulp of rat's molar", Clin Oral Investig, 4, pp.110~119 (2000)
- Six N, "Differential repair responses in the coronal and radicular areas of the exposed rat molar pulp induced by recombinant human bone morphogenetic protein 7 (osteogenic protein 1)", Arch Oral Biol, 47, pp.177~187 (2002)
- Louwakul P, "Response of inflamed pulps of rat molars after capping with pulp-capping material containing fluocinolone acetonide", J Endod, 41, pp.508~512 (2015)
- Dammaschke T, "A histologic investigation of direct pulp capping in rodents with dentin adhesives and calcium hydroxide", Quintessence Int, 41, pp.e62~e71 (2010)
- Hayashi K, "The possibility of genistein as a new direct pulp capping agent", Dent Mater J, 32, pp.976~985 (2013)
- Liu S, "Evaluation of a bioceramic as a pulp capping agent in vitro and in vivo", J Endod, 41, pp.652~657 (2015)
- Long Y, "Evaluation of pulp response to novel bioactive glass pulp capping materials", J Endod, 43, pp.1647~1650 (2017)
- Kawashima S, "Effect of an experimental adhesive resin containing multi-ion releasing fillers on direct pulp-capping", Dent Mater J, 35, pp.479~489 (2016)
- Suzuki M, "Histological evaluation of direct pulp capping of rat pulp with experimentally developed low-viscosity adhesives containing reparative dentin-promoting agents", J Dent, 44, pp.27~36 (2016)
- Berman DS, "Experimental pulpotomies in rat molars", J Dent Res, 37, pp.229~242 (1958)
- Paranjpe A, "Effects of mineral trioxide aggregate on human dental pulp cells after pulp-capping procedures", J Endod, 36, pp.1042~1047 (2010)
- Takita T, "Effect of mineral trioxide aggregate on proliferation of cultured human dental pulp cells", Int Endod J, 39, pp.415~422 (2006)
- Ford TR, "Using mineral trioxide aggregate as a pulp-capping material", J Am Dent Assoc, 127, pp.1491~1494 (1996)
- Faraco IM Jr, "Response of the pulp of dogs to capping with mineral trioxide aggregate or a calcium hydroxide cement", Dent Traumatol, 17, pp.163~166 (2001)
- Nair PN, "Histological, ultrastructural and quantitative investigations on the response of healthy human pulps to experimental capping with mineral trioxide aggregate: a randomized controlled trial", Int Endod J, 41, pp.128~150 (2008)
- 윤영란, "Mineral trioxide aggregate, calcium sulfate와 calcium hydroxide의 치수에 대한 반응", Restorative Dentistry and Endodontics, 32(2), pp.95~101 (2007), 대한치과보존학회
- Settawacharawanich S, "The chemical compositions and physicochemical properties of a Thai white Portland cement", Master's, pp.2006
- Pisalchaiyong N, "Evaluating cytotoxicity of Thai white Portland cement in cell culture using MTT assay", Mahidol Dent J, 30, pp.17~26 (2010)
- Chaimanakarn C, "Subcutaneous tissue response to calcium silicate-based cement", Master's, pp.2014
- Warotamawichaya S, "Effect of calcium chloride on setting time of Thai white Portland cement", Master's, pp.2011
- "ProRoot® MTA (Mineral Trioxide Aggregate) Root canal repair material" (2005)[URL]
- D'Souza RN, "Characterization of cellular responses involved in reparative dentinogenesis in rat molars", J Dent Res, 74, pp.702~709 (1995)
- Cotton WR, "Bacterial contamination as a factor in healing of pulp exposures", Oral Surg Oral Med Oral Pathol, 38, pp.441~450 (1974)
- Heys DR, "Healing of primate dental pulps capped with Teflon", Oral Surg Oral Med Oral Pathol, 69, pp.227~237 (1990)
- Cvek M, "Hard tissue barrier formation in pulpotomized monkey teeth capped with cyanoacrylate or calcium hydroxide for 10 and 60 minutes", J Dent Res, 66, pp.1166~1174 (1987)
- Pereira JC, "Pulp capping : influence of the exposure site on pulp healing--histologic and radiographic study in dogs' pulp", J Endod, 7, pp.213~223 (1981)
- Tziafas D, "Short-term dentinogenic response of dog dental pulp tissue after its induction by demineralized or native dentine, or predentine", Arch Oral Biol, 37, pp.119~128 (1992)
- Camilleri J, "Characterization of hydration products of mineral trioxide aggregate", Int Endod J, 41, pp.408~417 (2008)
- 장석우, "Chemical characteristics of mineral trioxide aggregate and its hydration reaction", Restorative Dentistry and Endodontics, 37(4), pp.188~193 (2012), 대한치과보존학회[DOI]
- Yasuda Y, "The effect of mineral trioxide aggregate on the mineralization ability of rat dental pulp cells : an in vitro study", J Endod, 34, pp.1057~1060 (2008)