Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with synthetic cell binding peptide sequences
1
조회
0
다운로드
4
인용
0
참고문헌
키워드
초록
Purpose: The aim of this study was to evaluate the improvement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with synthetic cell-binding peptide sequences in a standardized rabbit sinus model. Methods: Standardized 6-mm diameter defects were created bilaterally on the maxillary sinus of ten male New Zealand white rabbits, receiving BCP bone substitute coated with synthetic cell binding peptide sequences on one side (experimental group) and BCP bone substitute without coating (control group) on the other side. Histologic and histomorphometric analysis of bone formation was carried out after a healing period of 4 or 8 weeks. Results: Histological analysis revealed signs of new bone formation in both experimental groups (4- and 8-week healing groups) with a statistically significant increase in bone formation in the 4-week healing group compared to the control group. However, no statistically significant difference in bone formation was found between the 8-week healing group and the control group. Conclusions: This study found that BCP bone substitute coated with synthetic cell-binding peptide sequences enhanced osteoinductive potential in a standardized rabbit sinus model and its effectiveness was greater in the 4-week healing group than in the 8-week healing group.
Abstract
Purpose: The aim of this study was to evaluate the improvement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with synthetic cell-binding peptide sequences in a standardized rabbit sinus model. Methods: Standardized 6-mm diameter defects were created bilaterally on the maxillary sinus of ten male New Zealand white rabbits, receiving BCP bone substitute coated with synthetic cell binding peptide sequences on one side (experimental group) and BCP bone substitute without coating (control group) on the other side. Histologic and histomorphometric analysis of bone formation was carried out after a healing period of 4 or 8 weeks. Results: Histological analysis revealed signs of new bone formation in both experimental groups (4- and 8-week healing groups) with a statistically significant increase in bone formation in the 4-week healing group compared to the control group. However, no statistically significant difference in bone formation was found between the 8-week healing group and the control group. Conclusions: This study found that BCP bone substitute coated with synthetic cell-binding peptide sequences enhanced osteoinductive potential in a standardized rabbit sinus model and its effectiveness was greater in the 4-week healing group than in the 8-week healing group.
참고문헌 (36)
- Klijn RJ, "A meta-analysis of histomorphometric results and graft healing time of various biomaterials compared to autologous bone used as sinus floor augmentation material in humans", Tissue Eng Part B Rev, 16, pp.493~507 (2010)
- Klijn RJ, "Sinus floor augmentation surgery using autologous bone grafts from various donor sites: a meta-analysis of the total bone volume", Tissue Eng Part B Rev, 16, pp.295~303 (2010)
- Schlegel KA, "Changes of mineralization of free autogenous bone grafts used for sinus floor elevation", Clin Oral Implants Res, 17, pp.673~678 (2006)
- Schmitt CM, "Histological results after maxillary sinus augmentation with Straumann® BoneCeramic, Bio-Oss®, Puros®, and autologous bone. A randomized controlled clinical trial", Clin Oral Implants Res (2012)
- Frenken JW, "The use of Straumann Bone Ceramic in a maxillary sinus floor elevation procedure: a clinical, radiological, histological and histomorphometric evaluation with a 6-month healing period", Clin Oral Implants Res, 21, pp.201~208 (2010)
- Cordaro L, "Maxillary sinus grafting with Bio-Oss or Straumann Bone Ceramic: histomorphometric results from a randomized controlled multicenter clinical trial", Clin Oral Implants Res, 19, pp.796~803 (2008)
- Kim S, "Effects of biphasic calcium phosphate bone substitute on circumferential bone defects around dental implants in dogs", Int J Oral Maxillofac Implants, 26, pp.265~273 (2011)
- Daculsi G, "Current state of the art of biphasic calcium phosphate bioceramics", J Mater Sci Mater Med, 14, pp.195~200 (2003)
- Kim JW, "Bone formation of block and particulated biphasic calcium phosphate lyophilized with Escherichia coli-derived recombinant human bone morphogenetic protein 2 in rat calvarial defects", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 112, pp.298~306 (2011)
- Park JC, "Induction of bone formation by Escherichia coli-expressed recombinant human bone morphogenetic protein-2 using block-type macroporous biphasic calcium phosphate in orthotopic and ectopic rat models", J Periodontal Res, 46, pp.682~690 (2011)
- Wong DA, "Neurologic impairment from ectopic bone in the lumbar canal: a potential complication of off-label PLIF", TLIF use of bone morphogenetic protein-2 (BMP-2), 2008, pp.8, 1011~1018
- Kaneko H, "Direct stimulation of osteoclastic bone resorption by bone morphogenetic protein (BMP)-2 and expression of BMP receptors in mature osteoclasts", Bone, 27, pp.479~486 (2000)
- Smucker JD, "Increased swelling complications associated with off-label usage of rhBMP-2 in the anterior cervical spine", Spine, 31, pp.2813~2819 (2006)
- Park JB, "Osteopromotion with synthetic oligopeptide-coated bovine bone mineral in vivo", J Periodontol, 78, pp.157~163 (2007)
- 박준범, "Enhanced ridge preservation by bone mineral bound with collagen-binding synthetic oligopeptide: a clinical and histologic study in humans.", Journal of periodontology, 82(3), pp.471~480, American Academy of Periodontology
- Kim TI, "Biomimetic approach on human periodontal ligament cells using synthetic oligopeptides", J Periodontol, 75, pp.925~932 (2004)
- Hagel-Bradway S, "Regulation of bone cell metabolism", J Oral Pathol Med, 18, pp.344~351 (1989)
- Seyedin SM, "Osteoinduction: a report on the discovery and research of unique protein growth factors mediating bone development", Oral Surg Oral Med Oral Pathol, 68(4), pp.527~529 (1989)
- Hole BB, "A study of biologically active peptide sequences (P-15) on the surface of an ABM scaffold (PepGen P-15) using AFM and FTIR", J Biomed Mater Res A, 74, pp.712~721 (2005)
- Qian JJ, "Enhanced cell attachment to anorganic bone mineral in the presence of a synthetic peptide related to collagen", J Biomed Mater Res, 31, pp.545~554 (1996)
- Hanks T, "Comparison of cell viability on anorganic bone matrix with or without P-15 cell binding peptide", Biomaterials, 25, pp.4831~4836 (2004)
- Yukna RA, "Multi-center clinical evaluation of combination anorganic bovine-derived hydroxyapatite matrix (ABM)", cell binding peptide (P-15) as a bone replacement graft material in human periodontal osseous defects. 6-month results, 1998, pp.69, 655~663
- Ijiri S, "Effect of sterilization on bone morphogenetic protein", J Orthop Res, 12, pp.628~636 (1994)
- Asai S, "Maxillary sinus augmentation model in rabbits: effect of occluded nasal ostium on new bone formation", Clin Oral Implants Res, 13, pp.405~409 (2002)
- Seol YJ, "Enhanced osteogenic promotion around dental implants with synthetic binding motif mimicking bone morphogenetic protein (BMP)-2", J Biomed Mater Res A, 77, pp.599~607 (2006)
- Chen D, "Bone morphogenetic proteins", Growth Factors, 22, pp.233~241 (2004)
- Hwang CJ, "Immunogenicity of bone morphogenetic proteins", J Neurosurg Spine, 10, pp.443~451 (2009)
- Shimer AL, "Spinal reconstruction and bone morphogenetic proteins: open questions", Injury, 40(3), pp.S32~S38 (2009)
- Saito A, "Repair of 20-mm long rabbit radial bone defects using BMP-derived peptide combined with an alpha-tricalcium phosphate scaffold", J Biomed Mater Res A, 77, pp.700~706 (2006)
- Suzuki Y, "Alginate hydrogel linked with synthetic oligopeptide derived from BMP-2 allows ectopic osteoinduction in vivo", J Biomed Mater Res, 50, pp.405~409 (2000)
- Lee CK, "Biomimetic surface modification using synthetic oligopeptides for enhanced guided bone regeneration in beagles", J Periodontol, 83, pp.101~110 (2012)
- De Souza Nunes LS, "Immunoexpression of Cbfa-1", Runx2 and VEGF in sinus lift procedures using bone substitutes in rabbits, 2010, pp.21, 584~590
- Roberts EG, "Bone Physiology and Metabolism, In Contemporary implant dentistry", pp.557~598 (1994), Mosby Year Book
- Bhatnagar RS, "The role in cell binding of a beta-bend within the triple helical region in collagen alpha 1 (I) chain: structural and biological evidence for conformational tautomerism on fiber surface", J Biomol Struct Dyn, 14, pp.547~560 (1997)
- Valentin AH, "Receptor technology--cell binding to P-15: a new method of regenerating bone quickly and safely-preliminary histomorphometrical and mechanical results in sinus floor augmentations", Keio J Med, 53, pp.166~171 (2004)
- Bhatnagar RS, "Biomimetic habitats for cells: ordered matrix deposition and differentiation in gingival fibroblasts cultured on hydroxyapatitie coated with a collagen analogue", Cell Mater, 9, pp.93~104 (1999)
