본문 바로가기
KERD
논문KCI 등재

Improvement of the osteogenic potential of ErhBMP-2-/EGCG-coated biphasic calcium phosphate bone substitute: in vitro and in vivo activity

1

조회

0

다운로드

0

인용

0

참고문헌

키워드

주제분류치의학

초록

Purpose: The aim of this study was to evaluate the enhancement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). Methods: The cell viability, differentiation, and mineralization of osteoblasts was tested with ErhBMP-2-/EGCG solution. Coated BCP surfaces were also investigated. Standardized, 6-mm diameter defects were created bilaterally on the maxillary sinus of 10 male New Zealand white rabbits. After removal of the bony windows and elevation of sinus membranes, ErhBMP-2-/EGCG-coated BCP was applied on one defect in the test group. BCP was applied on the other defect to form the control group. The animals were sacrificed at 4 or 8 weeks after surgery. Histologic and histometric analyses of the augmented graft and surrounding tissue were performed. Results: The 4-week and 8-week test groups showed more new bone (%) than the corresponding control groups (P<0.05). The 8-week test group showed more new bone (%) than the 4-week test group (P<0.05). Conclusions: ErhBMP-2-/EGCG-coated BCP was effective as a bone graft material, showing enhanced osteogenic potential and minimal side effects in a rabbit sinus augmentation model.

Abstract

Purpose: The aim of this study was to evaluate the enhancement of osteogenic potential of biphasic calcium phosphate (BCP) bone substitute coated with Escherichia coli-derived recombinant human bone morphogenetic protein-2 (ErhBMP-2) and epigallocatechin-3-gallate (EGCG). Methods: The cell viability, differentiation, and mineralization of osteoblasts was tested with ErhBMP-2-/EGCG solution. Coated BCP surfaces were also investigated. Standardized, 6-mm diameter defects were created bilaterally on the maxillary sinus of 10 male New Zealand white rabbits. After removal of the bony windows and elevation of sinus membranes, ErhBMP-2-/EGCG-coated BCP was applied on one defect in the test group. BCP was applied on the other defect to form the control group. The animals were sacrificed at 4 or 8 weeks after surgery. Histologic and histometric analyses of the augmented graft and surrounding tissue were performed. Results: The 4-week and 8-week test groups showed more new bone (%) than the corresponding control groups (P<0.05). The 8-week test group showed more new bone (%) than the 4-week test group (P<0.05). Conclusions: ErhBMP-2-/EGCG-coated BCP was effective as a bone graft material, showing enhanced osteogenic potential and minimal side effects in a rabbit sinus augmentation model.

참고문헌 (30)

  1. Bessho K, "Bone induction by Escherichia coli-derived recombinant human bone morphogenetic protein-2 compared with Chinese hamster ovary cell-derived recombinant human bone morphogenetic protein-2", Br J Oral Maxillofac Surg, 38, pp.645~649 (2000)
  2. Lee JH, "The induction of bone formation in rat calvarial defects and subcutaneous tissues by recombinant human BMP-2, produced in Escherichia coli", Biomaterials, 31, pp.3512~3519 (2010)
  3. Long S, "Expression, purification, and renaturation of bone morphogenetic protein-2 from Escherichia coli", Protein Expr Purif, 46, pp.374~378 (2006)
  4. Vallejo LF, "Renaturation and purification of bone morphogenetic protein-2 produced as inclusion bodies in high-cell-density cultures of recombinant Escherichia coli", J Biotechnol, 94, pp.185~194 (2002)
  5. 최경희, "E. coli 발현 시스템에 의해 생산된 recombinant human bone morphogenetic protein-2의 정제와 생물학적 활성", Journal of Periodontal & Implant Science, 38(1), pp.41~50 (2008), 대한치주과학회
  6. Wong DA, "Neurologic impairment from ectopic bone in the lumbar canal: a potential complication of off-label PLIF", TLIF use of bone morphogenetic protein-2 (BMP2), 2008, pp.8, 1011~1018
  7. Kaneko H, "Direct stimulation of osteoclastic bone resorption by bone morphogenetic protein (BMP)-2 and expression of BMP receptors in mature osteoclasts", Bone, 27, pp.479~486 (2000)
  8. Smucker JD, "Increased swelling complications associated with offlabel usage of rhBMP-2 in the anterior cervical spine", Spine(Phila Pa 1976), 31, pp.2813~2819 (2006)
  9. 최현민, "Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with two concentrations of expressed recombinant human bone morphogenetic protein 2", Journal of Periodontal & Implant Science, 42(4), pp.119~126 (2012), 대한치주과학회[DOI]
  10. Yang CS, "Effects of tea consumption on nutrition and health", J Nutr, 130, pp.2409~2412 (2000)
  11. Tosetti F, "Metabolic regulation and redox activity as mechanisms for angioprevention by dietary phytochemicals", Int J Cancer, 125, pp.1997~2003 (2009)
  12. Rahman I, "Regulation of inflammation and redox signaling by dietary polyphenols", Biochem Pharmacol, 72, pp.1439~1452 (2006)
  13. Nakagawa H, "Fenton reaction is primarily involved in a mechanism of (-)-epigallocatechin-3-gallate to induce osteoclastic cell death", Biochem Biophys Res Commun, 292, pp.94~101 (2002)
  14. Yun JH, "Inhibitory effects of green tea polyphenol (-)-epigallocatechin gallate on the expression of matrix metalloproteinase-9 and on the formation of osteoclasts", J Periodontal Res, 39, pp.300~307 (2004)
  15. Vali B, "Epigallocatechin-3-gallate increases the formation of mineralized bone nodules by human osteoblast-like cells", J Nutr Biochem, 18, pp.341~347 (2007)
  16. Tokuda H, "(-)-Epigallocatechin gallate inhibits basic fibroblast growth factor-stimulated interleukin-6 synthesis in osteoblasts", Horm Metab Res, 40, pp.674~678 (2008)
  17. Tokuda H, "(--)-Epigallocatechin gallate enhances prostaglandin F2alpha-induced VEGF synthesis via upregulating SAPK", JNK activation in osteoblasts, 2007, pp.100, 1146~1153
  18. Kato K, "(-)-Epigallocatechin gallate inhibits thyroid hormone-stimulated osteocalcin synthesis in osteoblasts", Mol Med Rep, 4, pp.297~300 (2011)
  19. Kim S, "Effects of biphasic calcium phosphate bone substitute on circumferential bone defects around dental implants in dogs", Int J Oral Maxillofac Implants, 26, pp.265~273 (2011)
  20. Daculsi G, "Current state of the art of biphasic calcium phosphate bioceramics", J Mater Sci Mater Med, 14, pp.195~200 (2003)
  21. 최현민, "Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with synthetic cell binding peptide sequences", Journal of Periodontal & Implant Science, 42(5), pp.166~172 (2012), 대한치주과학회
  22. Shin YS, "The effects of ErhBMP-2-", EGCG-coated BCP bone substitute on dehiscence around dental implants in dogs, 2014, pp.20, 281~287
  23. Xiao SJ, "Covalent attachment of cell-adhesive, (Arg-Gly-Asp)-containing peptides to titanium surfaces", Langmuir, 14, pp.5507~5516 (1998)
  24. Durrieu MC, "Grafting RGD containing peptides onto hydroxyapatite to promote osteoblastic cells adhesion", J Mater Sci Mater Med, 15, pp.779~786 (2004)
  25. Choi Y, "Sinus augmentation using absorbable collagen sponge loaded with Escherichia coli-expressed recombinant human bone morphogenetic protein 2 in a standardized rabbit sinus model: a radiographic and histologic analysis", Clin Oral Implants Res, 23, pp.682~689 (2012)
  26. Misch CE, "Contemporary implant dentistry" (1999), Mosby
  27. Yun JH, "(-)-Epigallocatechin gallate induces apoptosis, via caspase activation, in osteoclasts differentiated from RAW 264.7 cells", J Periodontal Res, 42, pp.212~218 (2007)
  28. Xu H, "Histomorphometric study of the stability of newly formed bone after elevation of the floor of the maxillary sinus", Br J Oral Maxillofac Surg, 43, pp.493~499 (2005)
  29. Asai S, "Maxillary sinus augmentation model in rabbits: effect of occluded nasal ostium on new bone formation", Clin Oral Implants Res, 13, pp.405~409 (2002)
  30. Frenken JW, "The use of Straumann Bone Ceramic in a maxillary sinus floor elevation procedure: a clinical, radiological, histological and histomorphometric evaluation with a 6-month healing period", Clin Oral Implants Res, 21, pp.201~208 (2010)

관련 논문AI추천

Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with two concentrations of expressed recombinant human bone morphogenetic protein 2김성태, 박노재, 박영범, 오승한, 최경희, 최현민, 홍민호, Otgonbold Jamiyandorj대한치주과학회 · Journal of Periodontal & Implant Science2012 · 제42권 제4호Increased osteoinductivity and mineralization by minimal concentration of bone morphogenetic protein-2 loaded onto biphasic calcium phosphate in a rabbit sinus김재신, 이중석, 조규성, 차재국, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2016 · 제46권 제5호Improvement of osteogenic potential of biphasic calcium phosphate bone substitute coated with synthetic cell binding peptide sequences김성태, 박노재, 박영범, 오승한, 최현민, 홍민호, Otgonbold Jamiyandorj대한치주과학회 · Journal of Periodontal & Implant Science2012 · 제42권 제5호Comparative analysis of carrier systems for delivering bone morphogenetic proteins이중석, 임현창, 정의원, 정임희, 최성호대한치주과학회 · Journal of Periodontal & Implant Science2015 · 제45권 제4호Four-week histologic evaluation of grafted calvarial defects with adjunctive hyperbaric oxygen therapy in rats김성태, 오서은, 오승환, 장혜윤, 허경석대한치주과학회 · Journal of Periodontal & Implant Science2016 · 제46권 제4호Effects of Escherichia Coli-derived Recombinant Human Bone Morphogenetic Protein-2 Loaded Porous Hydroxyaptite-based Ceramics on Calvarial Defect in Rabbits김신영, 김용건, 서승준, 이영균, 임재홍대한골대사학회 · 대한골대사학회지2017 · 제24권 제1호

이 간행물의 다른 논문 (6건)