Increased osteoinductivity and mineralization by minimal concentration of bone morphogenetic protein-2 loaded onto biphasic calcium phosphate in a rabbit sinus
1
조회
0
다운로드
1
인용
0
참고문헌
키워드
초록
Purpose: The purpose of the present study was to evaluate the effectiveness of a minimal concentration of bone morphogenetic protein-2 (BMP-2) in terms of quantitative and qualitative analyses of newly formed bone in a rabbit maxillary sinus model. Methods: In 7 rabbits, sinus windows were prepared bilaterally. Biphasic calcium phosphate (BCP) loaded with 0.05 mg/mL BMP-2 was grafted into one sinus (the BMP group) and saline-soaked BCP was placed into the other (the control group) in each animal. The animals were allowed an 8-week healing period before being sacrificed. Specimens including the augmented area and surrounding tissues were then removed and evaluated both radiographically and histologically. Results: There was a difference in the mineralization of new bone between the groups. In the BMP group, the greater part of the new bone consisted of mature lamellar bone with an evident trabecular pattern, whereas the control group showed mostly woven bone, consisting only partially of lamellar bone. Histometrically, the area of new bone was significantly greater (4.55±1.35 mm2 vs. 2.99±0.86 mm2) in the BMP group than in the control group (P<0.05); however, the total augmentation volumes were not significantly different between the groups. Conclusions: Within the limitations of this study, it can be suggested that a minimal concentration of BMP-2 (0.05 mg/mL) had an osteoinductive effect with accelerated mineralization in a rabbit sinus model using a BCP carrier.
Abstract
Purpose: The purpose of the present study was to evaluate the effectiveness of a minimal concentration of bone morphogenetic protein-2 (BMP-2) in terms of quantitative and qualitative analyses of newly formed bone in a rabbit maxillary sinus model. Methods: In 7 rabbits, sinus windows were prepared bilaterally. Biphasic calcium phosphate (BCP) loaded with 0.05 mg/mL BMP-2 was grafted into one sinus (the BMP group) and saline-soaked BCP was placed into the other (the control group) in each animal. The animals were allowed an 8-week healing period before being sacrificed. Specimens including the augmented area and surrounding tissues were then removed and evaluated both radiographically and histologically. Results: There was a difference in the mineralization of new bone between the groups. In the BMP group, the greater part of the new bone consisted of mature lamellar bone with an evident trabecular pattern, whereas the control group showed mostly woven bone, consisting only partially of lamellar bone. Histometrically, the area of new bone was significantly greater (4.55±1.35 mm2 vs. 2.99±0.86 mm2) in the BMP group than in the control group (P<0.05); however, the total augmentation volumes were not significantly different between the groups. Conclusions: Within the limitations of this study, it can be suggested that a minimal concentration of BMP-2 (0.05 mg/mL) had an osteoinductive effect with accelerated mineralization in a rabbit sinus model using a BCP carrier.
참고문헌 (21)
- Kim JS, "Acceleration of bone regeneration by Bmp-2-loaded collagenated biphasic calcium phosphate in rabbit sinus", Clin Implant Dent Relat Res, 17, pp.1103~1113 (2015)
- LeGeros RZ, "Biphasic calcium phosphate bioceramics: preparation, properties and applications", J Mater Sci Mater Med, 14, pp.201~209 (2003)
- Lim HC, "Effect of different hydroxyapatite:beta-tricalcium phosphate ratios on the osteoconductivity of biphasic calcium phosphate in the rabbit sinus model", Int J Oral Maxillofac Implants, 30, pp.65~72 (2015)
- Mangano C, "A human clinical, histological, histomorphometrical, and radiographical study on biphasic HA-beta-TCP 30", 70 in maxillary sinus augmentation, 2015, pp.17, 610~618
- Choi Y, "Recombinant human bone morphogenetic protein-2 stimulates the osteogenic potential of the Schneiderian membrane: a histometric analysis in rabbits", Tissue Eng Part A, 19, pp.1994~2004 (2013)
- Asai S, "Maxillary sinus augmentation model in rabbits: effect of occluded nasal ostium on new bone formation", Clin Oral Implants Res, 13, pp.405~409 (2002)
- Brunner E, "Nonparametric analysis of ordered categorical data in designs with longitudinal observations and small sample sizes", Biom J, 42, pp.663~675 (2000)
- Torrecillas-Martínez L, "Significance of the immunohistochemical expression of bone morphogenetic protein-4 in bone maturation after maxillary sinus grafting in humans", Clin Implant Dent Relat Res, 18, pp.717~724 (2016)
- Hong JY, "A high concentration of recombinant human bone morphogenetic protein-2 induces low-efficacy bone regeneration in sinus augmentation: a histomorphometric analysis in rabbits", Clin Oral Implants Res (2015)
- Boden SD, "Use of recombinant human bone morphogenetic protein-2 to achieve posterolateral lumbar spine fusion in humans: a prospective, randomized clinical pilot trial: 2002 Volvo Award in clinical studies", Spine (Phila Pa 1976), 27, pp.2662~2673 (2002)
- Yon J, "Pre-clinical evaluation of the osteogenic potential of bone morphogenetic protein-2 loaded onto a particulate porcine bone biomaterial", J Clin Periodontol, 42, pp.81~88 (2015)
- Kim MS, "Low-dose recombinant human bone morphogenetic protein-2 to enhance the osteogenic potential of the Schneiderian membrane in the early healing phase: in vitro and in vivo studies", J Oral Maxillofac Surg, 72, pp.1480~1494 (2014)
- Kenley RA, "Biotechnology and bone graft substitutes", Pharm Res, 10, pp.1393~1401 (1993)
- Haidar ZS, "Delivery of recombinant bone morphogenetic proteins for bone regeneration and repair. Part B: delivery systems for BMPs in orthopaedic and craniofacial tissue engineering", Biotechnol Lett, 31, pp.1825~1835 (2009)
- Jung RE, "Bone morphogenetic protein-2 enhances bone formation when delivered by a synthetic matrix containing hydroxyapatite", tricalciumphosphate, 2008, pp.19, 188~195
- Kim JW, "Volumetric bone regenerative efficacy of biphasic calcium phosphate-collagen composite block loaded with rhBMP-2 in vertical bone augmentation model of a rabbit calvarium", J Biomed Mater Res A, 100, pp.3304~3313 (2012)
- Jang JW, "Osteoinductive activity of biphasic calcium phosphate with different rhBMP-2 doses in rats", Oral Surg Oral Med Oral Pathol Oral Radiol, 113, pp.480~487 (2012)
- 임현창, "Late-term healing in an augmented sinus with different ratios of biphasic calcium phosphate: a pilot study using a rabbit sinus model", Journal of Periodontal & Implant Science, 46(1), pp.57~69 (2016), 대한치주과학회
- Kim MS, "Prospective randomized, controlled trial of sinus grafting using Escherichia-coli-produced rhBMP-2 with a biphasic calcium phosphate carrier compared to deproteinized bovine bone", Clin Oral Implants Res, 26, pp.1361~1368 (2015)
- Park JC, "Dose- and time-dependent effects of recombinant human bone morphogenetic protein-2 on the osteogenic and adipogenic potentials of alveolar bone-derived stromal cells", J Periodontal Res, 47, pp.645~654 (2012)
- Choi Y, "Sinus augmentation using absorbable collagen sponge loaded with Escherichia coli-expressed recombinant human bone morphogenetic protein 2 in a standardized rabbit sinus model: a radiographic and histologic analysis", Clin Oral Implants Res, 23, pp.682~689 (2012)