Association between immunoglobulin G1 against Tannerella forsythia and reduction in the loss of attachment tissue
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Purpose: To evaluate whether the levels of immunoglobulin G (IgG) antibody to Tanerellaforsythia are associated with periodontal status. Methods: Patients with a diagnosis of chronic periodontitis were considered candidates forthe study; thus 80 chronic periodontitis patients and 28 healthy persons (control group)were invited to participate in this investigation. The presence of T. forsythia was detectedby polymerase chain reaction (PCR) analysis using primers designed to target the respective16S rRNA gene sequences. Peripheral blood was collected from each subject to identify theIgG1 and IgG2 serum antibodies against T. forsythia. All microbiological and immunologicallaboratory processes were completed blindly, without awareness of the clinical status ofthe study patients or of the periodontal sites tested. Results: The bivariate analysis showed that lower mean levels of clinical attachment level(CAL) and probing depth were found in the presence of the IgG1 antibody titers againstwhole-cell T. forsythia; however, only the difference in CAL was statistically significant. Inthe presence of the IgG2 antibody titers against whole-cell T. forsythia, the periodontal parametersevaluated were higher but they did not show statistical differences, except forplaque. The unadjusted linear regression model showed that the IgG1 antibody againstwhole-cell T. forsythia in periodontitis patients was associated with a lower mean CAL(β=–0.654; 95% confidence interval [CI], –1.27 to –0.28; P<0.05). This statistically significantassociation remained after adjusting for possible confounders (β=–0.655; 95% CI,–1.28 to –0.29; P<0.05). On the other hand, smoking was a statistically significant risk factorin the model (β=0.704; 95% CI, 0.24 to 1.38; P<0.05). Conclusions: Significantly lower mean levels of CAL were shown in the presence of theIgG1 antibody titers against whole-cell T. forsythia in periodontitis patients. Thus, the resultsof this study suggest that IgG1 antibody to T. forsythia may have been a protectivefactor from periodontitis in this sample.
Abstract
Purpose: To evaluate whether the levels of immunoglobulin G (IgG) antibody to Tanerellaforsythia are associated with periodontal status. Methods: Patients with a diagnosis of chronic periodontitis were considered candidates forthe study; thus 80 chronic periodontitis patients and 28 healthy persons (control group)were invited to participate in this investigation. The presence of T. forsythia was detectedby polymerase chain reaction (PCR) analysis using primers designed to target the respective16S rRNA gene sequences. Peripheral blood was collected from each subject to identify theIgG1 and IgG2 serum antibodies against T. forsythia. All microbiological and immunologicallaboratory processes were completed blindly, without awareness of the clinical status ofthe study patients or of the periodontal sites tested. Results: The bivariate analysis showed that lower mean levels of clinical attachment level(CAL) and probing depth were found in the presence of the IgG1 antibody titers againstwhole-cell T. forsythia; however, only the difference in CAL was statistically significant. Inthe presence of the IgG2 antibody titers against whole-cell T. forsythia, the periodontal parametersevaluated were higher but they did not show statistical differences, except forplaque. The unadjusted linear regression model showed that the IgG1 antibody againstwhole-cell T. forsythia in periodontitis patients was associated with a lower mean CAL(β=–0.654; 95% confidence interval [CI], –1.27 to –0.28; P<0.05). This statistically significantassociation remained after adjusting for possible confounders (β=–0.655; 95% CI,–1.28 to –0.29; P<0.05). On the other hand, smoking was a statistically significant risk factorin the model (β=0.704; 95% CI, 0.24 to 1.38; P<0.05). Conclusions: Significantly lower mean levels of CAL were shown in the presence of theIgG1 antibody titers against whole-cell T. forsythia in periodontitis patients. Thus, the resultsof this study suggest that IgG1 antibody to T. forsythia may have been a protectivefactor from periodontitis in this sample.
참고문헌 (28)
- Socransky SS, "Microbial complexes in subgingival plaque", J Clin Periodontol, 25, pp.134~144 (1998)
- Amano A, "Genetic characteristics and pathogenic mechanisms of periodontal pathogens", Adv Dent Res, 26, pp.15~22 (2014)
- Onishi H, "Levels of specific immunoglobulin G to the forsythia detaching factor of Tannerella forsythia in gingival crevicular fluid are related to the periodontal status", J Periodontal Res, 45, pp.672~680 (2010)
- Onishi H, "Modification of forsythia detaching factor by gingival crevicular fluid in periodontitis", Arch Oral Biol, 58, pp.1007~1013 (2013)
- Yoo JY, "Identification of Tannerella forsythia antigens specifically expressed in patients with periodontal disease", FEMS MICROBIOLOGY LETTERS, 275(2), pp.344~352 (2007), BLACKWELL PUBLISHING
- Hall LM, "Levels of serum immunoglobulin G specific to bacterial surface protein A of Tannerella forsythia are related to periodontal status", J Periodontol, 83, pp.228~234 (2012)
- Kinane DF, "Humoral immune response to Actinobacillus actinomycetemcomitans and Porphyromonas gingivalis in periodontal disease", Periodontol 2000, 20, pp.289~340 (1999)
- Haffajee AD, "Clinical, microbiological and immunological features of subjects with refractory periodontal diseases", J Clin Periodontol, 15, pp.390~398 (1988)
- Persson GR, "Serum antibody titers to Bacteroides forsythus in elderly subjects with gingivitis or periodontitis", J Clin Periodontol, 27, pp.839~845 (2000)
- Yoneda M, "Humoral immune responses to S-layer-like proteins of Bacteroides forsythus", Clin Diagn Lab Immunol, 10, pp.383~387 (2003)
- Vlachojannis C, "Determinants of serum IgG responses to periodontal bacteria in a nationally representative sample of US adults", J Clin Periodontol, 37, pp.685~696 (2010)
- Craig RG, "Serum IgG antibody response to periodontal pathogens in minority populations: relationship to periodontal disease status and progression", J Periodontal Res, 37, pp.132~146 (2002)
- Eke PI, "Update of the case definitions for population-based surveillance of periodontitis", J Periodontol, 83, pp.1449~1454 (2012)
- Ashimoto A, "Polymerase chain reaction detection of 8 putative periodontal pathogens in subgingival plaque of gingivitis and advanced periodontitis lesions", Oral Microbiol Immunol, 11, pp.266~273 (1996)
- Bishop RF, "Estimation of rotavirus immunoglobulin G antibodies in human serum samples by enzyme-linked immunosorbent assay: expression of results as units derived from a standard curve", J Clin Microbiol, 19, pp.447~452 (1984)
- Walker C, "An in vitro biofilm model of subgingival plaque", Oral Microbiol Immunol, 22, pp.152~161 (2007)
- Murayama Y, "Serum immunoglobulin G antibody to periodontal bacteria", Adv Dent Res, 2, pp.339~345 (1988)
- Aliprantis AO, "Cell activation and apoptosis by bacterial lipoproteins through toll-like receptor-2", Science, 285, pp.736~739 (1999)
- Raetz CR, "Lipopolysaccharide endotoxins", Annu Rev Biochem, 71, pp.635~700 (2002)
- Lee HR, "Tannerella forsythia BspA increases the risk factors for atherosclerosis in ApoE(-", -) mice, 2014, pp.20, 803~808
- Gemmell E, "The role of T cells in periodontal disease: homeostasis and autoimmunity", Periodontol 2000, 43, pp.14~40 (2007)
- Califano JV, "A comparison of IgG antibody reactive with Bacteroides forsythus and Porphyromonas gingivalis in adult and early-onset periodontitis", J Periodontol, 68, pp.734~738 (1997)
- Dye BA, "Serum antibodies to periodontal bacteria as diagnostic markers of periodontitis", J Periodontol, 80, pp.634~647 (2009)
- Guglielmetti MR, "Detection and quantification of periodontal pathogens in smokers and never-smokers with chronic periodontitis by real-time polymerase chain reaction", J Periodontol, 85, pp.1450~1457 (2014)
- Quinn SM, "The influence of smoking and race on adult periodontitis and serum IgG2 levels", J Periodontol, 69, pp.171~177 (1998)
- Chung HY, "Immunoglobulin G profiles in different forms of periodontitis", J Periodontal Res, 38, pp.471~476 (2003)
- Chen HA, "Humoral immune responses to Porphyromonas gingivalis before and following therapy in rapidly progressive periodontitis patients", J Periodontol, 62, pp.781~791 (1991)
- Whitney C, "Serum immunoglobulin G antibody to Porphyromonas gingivalis in rapidly progressive periodontitis: titer, avidity, and subclass distribution", Infect Immun, 60, pp.2194~2200 (1992)