본문 바로가기
KERD
논문KCI 등재

Removal efficacy and cytotoxicity of a calcium hydroxide paste using N-2-methyl-pyrrolidone as a vehicle

2

조회

0

다운로드

1

인용

0

참고문헌

키워드

주제분류치의학

초록

Objectives: This study investigated the removal efficacy and cytotoxicity of a newly developed calcium hydroxide paste (cleaniCal, Maruchi) using N-2-methyl-pyrrolidone (NMP) as a vehicle in comparison with ApexCal (Ivoclar Vivadent) and Calcipex II (Nishika), which use different vehicles such as polyethylene glycol and propylene glycol, respectively. Materials and Methods: Thirty maxillary premolars with oval-shaped canals were divided into 3 groups and the teeth were filled with one of the pastes. After removal of the paste, micro-computed tomographic (μ-CT) imaging was obtained to assess the volume of residual paste in the root canal of each tooth. The teeth were then split longitudinally and the area of the paste-coated surface was evaluated by stereomicroscopy. The cytotoxicity of each product was assessed using an agar overlay assay. The effect of each vehicle on cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed using one-way analysis of variance and Tukey's tests to detect any significance (p < 0.05). Results: In the μ-CT and stereomicroscopic analysis, cleaniCal exhibited less remnants of medicament than ApexCal and Calcipex. cleaniCal showed a higher cytotoxicity than the other pastes in the agar overlay assay. Furthermore, NMP exhibited lower cell viability compared to the other vehicles. Conclusions: cleaniCal showed better removal efficacy compared to the other products. However, clinicians should be aware of the higher cytotoxicity of the NMP-based material and consider its possible adverse effects on periradicular tissue when it is overfilled.

Abstract

Objectives: This study investigated the removal efficacy and cytotoxicity of a newly developed calcium hydroxide paste (cleaniCal, Maruchi) using N-2-methyl-pyrrolidone (NMP) as a vehicle in comparison with ApexCal (Ivoclar Vivadent) and Calcipex II (Nishika), which use different vehicles such as polyethylene glycol and propylene glycol, respectively. Materials and Methods: Thirty maxillary premolars with oval-shaped canals were divided into 3 groups and the teeth were filled with one of the pastes. After removal of the paste, micro-computed tomographic (μ-CT) imaging was obtained to assess the volume of residual paste in the root canal of each tooth. The teeth were then split longitudinally and the area of the paste-coated surface was evaluated by stereomicroscopy. The cytotoxicity of each product was assessed using an agar overlay assay. The effect of each vehicle on cell viability was evaluated using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The data were analyzed using one-way analysis of variance and Tukey's tests to detect any significance (p < 0.05). Results: In the μ-CT and stereomicroscopic analysis, cleaniCal exhibited less remnants of medicament than ApexCal and Calcipex. cleaniCal showed a higher cytotoxicity than the other pastes in the agar overlay assay. Furthermore, NMP exhibited lower cell viability compared to the other vehicles. Conclusions: cleaniCal showed better removal efficacy compared to the other products. However, clinicians should be aware of the higher cytotoxicity of the NMP-based material and consider its possible adverse effects on periradicular tissue when it is overfilled.

참고문헌 (33)

  1. Bystrom A, "The antibacterial effect of camphorated paramonochlorophenol, camphorated phenol and calcium hydroxide in the treatment of infected root canals", Endod Dent Traumatol, 1, pp.170~175 (1985)
  2. Calt S, "Dentinal tubule penetration of root canal sealers after root canal dressing with calcium hydroxide", J Endod, 25, pp.431~433 (1999)
  3. Hosoya N, "Effects of calcium hydroxide on physical and sealing properties of canal sealers", Int Endod J, 37, pp.178~184 (2004)
  4. Guiotti FA, "Effect of calcium hydroxide dressing on push-out bond strength of endodontic sealers to root canal dentin", Braz Oral Res, 28, pp.1~6 (2014)
  5. Grover C, "Evaluation of calcium ion release and change in pH on combining calcium hydroxide with different vehicles", Contemp Clin Dent, 5, pp.434~439 (2014)
  6. Gomes BP, "Microbial susceptibility to calcium hydroxide pastes and their vehicles", J Endod, 28, pp.758~761 (2002)
  7. Simi Junior J, "Histological analysis of the biocompatibility of calcium hydroxide associated with a new vehicle", Indian J Dent Res, 26, pp.627~632 (2015)
  8. Simon ST, "Effect of four vehicles on the pH of calcium hydroxide and the release of calcium ion", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 80, pp.459~464 (1995)
  9. Camargo CH, "Vehicle influence on calcium hydroxide pastes diffusion in human and bovine teeth", Dent Traumatol, 22, pp.302~306 (2006)
  10. Deonizio MD, "Efficacy of calcium hydroxide paste prepared with different vehicles against salivary microbial infiltration of root canals", Gen Dent, 62, pp.e22~e25 (2014)
  11. Cruz EV, "Penetration of propylene glycol into dentine", Int Endod J, 35, pp.330~336 (2002)
  12. Camões IC, "Influence on pH of vehicle containing glycerin used with calcium hydroxide", Dent Traumatol, 19, pp.132~138 (2003)
  13. Balvedi RP, "A comparison of two techniques for the removal of calcium hydroxide from root canals", Int Endod J, 43, pp.763~768 (2010)
  14. Lloyd A, "Removal of calcium hydroxide from Weine Type II systems using photon-induced photoacoustic streaming, passive ultrasonic, and needle irrigation: a microcomputed tomography study", J Appl Oral Sci, 24, pp.543~548 (2016)
  15. da Silva JM, "Microscopic cleanliness evaluation of the apical root canal after using calcium hydroxide mixed with chlorhexidine, propylene glycol, or antibiotic paste", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 111, pp.260~264 (2011)
  16. Hamishehkar H, "The relationship between solubility and transdermal absorption of Tadalafil", Adv Pharm Bull, 5, pp.411~417 (2015)
  17. Kenee DM, "A quantitative assessment of efficacy of various calcium hydroxide removal techniques", J Endod, 32, pp.563~565 (2006)
  18. Sanghvi R, "Solubility improvement of drugs using N-methyl pyrrolidone", AAPS PharmSciTech, 9, pp.366~376 (2008)
  19. Jain P, "Solubilization of poorly soluble compounds using 2-pyrrolidone", Int J Pharm, 342, pp.1~5 (2007)
  20. Li Q, "N-methyl pyrrolidone (NMP) ameliorates the hypoxia-reduced osteoblast differentiation via inhibiting the NF-κB signaling", J Toxicol Sci, 41, pp.701~709 (2016)
  21. Aboudzadeh N, "Fabrication and characterization of poly(D,L-lactide-co-glycolide)", hydroxyapatite nanocomposite scaffolds for bone tissue regeneration, 2010, pp.94, 137~145
  22. Sasaki H, "Enhancing effect of pyrrolidone derivatives on transdermal penetration of 5-fluorouracil, triamcinolone acetonide, indomethacin, and flurbiprofen", J Pharm Sci, 80, pp.533~538 (1991)
  23. Agrawal AG, "Toxicity Study of a Self-nanoemulsifying Drug Delivery System Containing N-methyl pyrrolidone", Drug Res (Stuttg), 65, pp.446~448 (2015)
  24. Gjoksi B, "Influence of N-methyl pyrrolidone on the activity of the pulp-dentine complex and bone integrity during osteoporosis", Int Endod J, 50, pp.271~280 (2017)
  25. Karfeld-Sulzer LS, "N-methyl pyrrolidone", bone morphogenetic protein-2 double delivery with in situ forming implants, 2015, pp.203, 181~188
  26. Leira HL, "Irritant cutaneous reactions to N-methyl-2-pyrrolidone (NMP)", Contact Dermatitis, 27, pp.148~150 (1992)
  27. Flick B, "Embryotoxic potential of N-methyl-pyrrolidone (NMP) and three of its metabolites using the rat whole embryo culture system", Toxicol Appl Pharmacol, 237, pp.154~167 (2009)
  28. Kim JW, "Overfilling of calcium hydroxide-based paste Calcipex II produced a foreign body granuloma without acute inflammatory reaction", Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 107, pp.e73~e76 (2009)
  29. 김진우, "Chronic maxillary sinusitis caused by root canal overfilling of Calcipex II", Restorative Dentistry and Endodontics, 39(1), pp.63~67 (2014), 대한치과보존학회[DOI]
  30. Asheibi F, "Micro-CT evaluation of the effectiveness of the combined use of rotary and hand instrumentation in removal of Resilon", Dent Mater J, 33, pp.1~6 (2014)
  31. Wiseman A, "Efficacy of sonic and ultrasonic activation for removal of calcium hydroxide from mesial canals of mandibular molars: a microtomographic study", J Endod, 37, pp.235~238 (2011)
  32. Silva LJ, "Micro-CT evaluation of calcium hydroxide removal through passive ultrasonic irrigation associated with or without an additional instrument", Int Endod J, 48, pp.768~773 (2015)
  33. Ma JZ, "Micro-computed tomography evaluation of the removal of calcium hydroxide medicament from C-shaped root canals of mandibular second molars", Int Endod J, 48, pp.333~341 (2015)

관련 논문AI추천

Calcium-doped zinc oxide nanocrystals as an innovative intracanal medicament: a pilot studyBarbosa Gabriella Lopes de Rezende, de Souza Gabriela Leite, Freitas Gabrielle Alves Nunes, Lemus Nelly Xiomara Alvarado, Magalhães Thamara Eduarda Alves, Moura Camilla Christian Gomes, Silva Anielle Christine Almeida대한치과보존학회 · Restorative Dentistry and Endodontics2022 · 제47권 제4호Antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: a literature review - Part II. in vivo studies김도현, 김의성대한치과보존학회 · Restorative Dentistry and Endodontics2015 · 제40권 제2호Calcium hydroxide dressing residues after different removal techniques affect the accuracy of Root-ZX apex locatorAyhan Eymirli, Emel Uzunoglu, Emre Nagas, Mehmet Özgür Uyanik, Semra Çalt대한치과보존학회 · Restorative Dentistry and Endodontics2015 · 제40권 제1호Efficacy of various irrigation methods for the removal of calcium hydroxide paste in the root canal민정범, 이의중, 조형훈, 황호길치의학연구원 · Oral Biology Research2016 · 제40권 제4호Antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: a literature review - Part I. In vitro studies김도현, 김의성대한치과보존학회 · Restorative Dentistry and Endodontics2014 · 제39권 제4호Physicochemical properties, cytotoxicity and penetration into dentinal tubules of sodium hypochlorite with and without surfactantsBarbieri Isadora, Coaguila-Llerena Hernán, Faria Gisele, Leonardo Renato de Toledo, Ramos Ana Paula, Tanomaru-Filho Mário대한치과보존학회 · Restorative Dentistry and Endodontics2020 · 제45권 제4호

이 간행물의 다른 논문 (10건)