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Nicotinamide의 투여가 일시적인 국소허혈로 유발된 흰쥐의 뇌경색에 미치는 영향

Nicotinamide Reduces the Infarct Volume in a Rat Model of Transient Middle Cerebral Artery Occlusion

강구|정일영|안영준|김근우|강성식|이건재|최기용|이민섭
대한병리학회|2006.04|Journal of Pathology and Translational Medicine|제40권 제2호|pp. 93-102

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참고문헌

주제분류병리학

초록

Abstract

Background : Cerebral ischemia depletes ATP and causes irreversible tissue injury. Nicotinamide is a precursor of NAD+ and it is also a poly (ADP-ribose) polymerase (PARP) inhibitor that increases the neuronal ATP concentration and so protects against stroke. Therefore we examined whether nicotinamide could protect against cerebral ischemia by using a model of transient middle cerebral artery occlusion (MCAO) (reperfusion 2 h post ischemia) in Sprague- Dawley rats. Methods : Nicotinamide (500 mg/kg) or normal saline was administered intraperitoneally 24 and 0 h before and after MCAO, respectively. The infarction volumes were determined with triphenyltetrazolium chloride staining 24 h after reperfusion. The nitrotyrosine, PAR polymer and PARP-1 expressions were examined by immunohistochemistry with using brain slices obtained from the rats that were sacrificed at 0, 15, 30, 60 and 120 min after reperfusion. Results : The infarction volumes were significantly attenuated (21.8%, p<0.05). The nitrotyrosine expressions were increased at 0, 15 and 30 min, and those expressions for PARP polymer and PARP-1 were increased at 60 and 120 min, respectively. Nicotinamide partly reduced the expressions for nitrotyrosine and PAR polymer except for PARP-1. Conclusions : These results suggest that nicotinamide may attenuate ischemic brain injury through its antioxidant activity and the inhibition of PARP-1.

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관련 논문AI추천

허혈성 뇌졸중 모델에서 허혈 및 재관류 손상에 대한 Poly (ADP-ribose) polymerase 억제제의 효과정부, 고성호, 권혁성, 김기석, 김명호, 김선정, 김승현, 김용순, 김주한, 박윤주, 송치원, 유현정, 정해관대한신경과학회 · 대한신경과학회지2003 · 제21권 제6호일시적 국소허혈에 의해 유발된 흰쥐 대뇌 신경세포 손상에 대한 EGCG (epigallocatechin gallate)의신경보호효과김범생, 박정욱, 이광수, 최영빈, 한시령대한신경과학회 · 대한신경과학회지2003 · 제21권 제4호흰쥐 대뇌의 국소허혈로 유발되는 신경세포의 세포사멸사(apoptosis)와Apoptosis Inducing Factor의 관련성에 관한 연구이영일대한체질인류학회 · 해부·생물인류학2003 · 제16권 제4호성향정기산이 흰쥐의 MCAO에 의한 국소뇌허혈에 미치는 영향김연섭, 김효선한의병리학회 · 동의생리병리학회지2006 · 제20권 제3호일시적 국소 뇌허혈손상에서 신경세포의 세포자멸사와 Inducible Nitric Oxide Synthase 발현강구성, 곽은경, 김정완, 박지영, 손윤경, 이병육, 이영미, 전홍화, 황성규대한병리학회 · Journal of Pathology and Translational Medicine2004 · 제38권 제6호허혈이 유도된 대뇌신경세포에 대한 항산화제 및 Ampa/kainate 수용체 길항제의 영향오연균한의병리학회 · 동의생리병리학회지2005 · 제19권 제4호

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