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허혈이 유도된 대뇌신경세포에 대한 항산화제 및 Ampa/kainate 수용체 길항제의 영향

Effect of Antioxidant and Ampa/kainate Receptor Antagonist on Cerebral Neurons Damaged by Ischemia

오연균
한의병리학회 (The Society of Pathology in Korean Medicine)|2005.08|동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)|제19권 제4호|pp. 1022-1026

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초록

Abstract

To clarify the toxic effect on cultured neonatal mouse cerebral neurons damaged by ischemia, we examined the cytotoxicity induced by ischemia and the protective effect of antioxidant and AMPA/kainate receptor antagonist against ischemia-induced cytotoxicity on cultured cerebral neurons. For this study, mice were administrated with 20ug/kg cyclothiazide or 50U/kg vitamin E via intraperitoneal injection for 2 hours before ischemic induction. After cell culture for 7 days, cell viability, amount of neurofilament and protein kinase C activity were examined. Ischemia decreased significantly cell viability, amount of neurofilament and the increase of protein kinase C activity in these cultures. In the protective effect, vitamin E showed remarkably the increase of cell viability and amount of neurofilament, and the decrease of protein kinase C activity but, cyclothiazide did not showed any protective effect on ischemia-induced cytotoxicity. From these results, it is suggested that vitamin E is effective in blocking the neurotoxicity induced by ischemia, but cyclothiazide as a AMPA/kainate receptor antagonist is not.

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