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일시적 국소 뇌허혈손상에서 신경세포의 세포자멸사와 Inducible Nitric Oxide Synthase 발현

Correlation Between Neuronal Apoptosis and Expression of Inducible Nitric Oxide Synthase after Transient Focal Cerebral Ischemia

박지영|황성규|이영미|김정완|곽은경|전홍화|손윤경|강구성|이병육
대한병리학회|2004.12|Journal of Pathology and Translational Medicine|제38권 제6호|pp. 364-371

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주제분류병리학

초록

Abstract

Background : Neuronal death in acute-phase cerebral ischemic injury is caused by necrosis. However, neuronal injury after reperfusion can be associated with apoptosis. Methods : We used Sprague-Dawley rats whose brains were reperfused after middle cerebral artery occlusion for either 30 min or 2 h. We examined a relationship between apoptosis and the expression of inducible nitric oxide synthase (iNOS) in the brain tissue from 3 h to 14 days after reperfusion in both groups. Results : TUNEL and iNOS positivity were closely related in both groups. The 2-h ischemia group exhibited increases in the amount of TUNEL and iNOS-positive cells for up to 3 days after reperfusion, at which the TUNEL and iNOS-positive cells decreased. The 30-min ischemia group exhibited peak positivity 24 h after reperfusion, followed by a similar decrease. iNOS mRNA expression peaked 3 h after reperfusion in the 30-min ischemia group, at which time it decreased. In the 2-h ischemia group, iNOS mRNA increased 3 h after reperfusion, peaked 24 h after reperfusion, and then decreased. Conclusions : These results indicated the occurrence of delayed apoptosis in transient cerebral ischemia. Increased expression of iNOS is closely associated with this apoptosis, and oxygen free radical-producing materials, such as nitric oxide, may play an important role in the induction of this apoptosis.

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관련 논문AI추천

흰쥐 대뇌의 국소허혈로 유발되는 신경세포의 세포사멸사(apoptosis)와Apoptosis Inducing Factor의 관련성에 관한 연구이영일대한체질인류학회 · 해부·생물인류학2003 · 제16권 제4호PC12 세포주에서 Lipopolysaccharide/Tumor Necrosis Factor-α/Interferon-γ로 유도된 Inducible Nitric Oxide Synthase 발현과 세포자멸사강구성, 곽은경, 김지연, 김지영, 박지영, 박태인, 손윤경대한병리학회 · Journal of Pathology and Translational Medicine2002 · 제36권 제4호백서 collagenase 유도 뇌출혈모델에서 Inducible nitric oxide synthase의 발현김성훈대한뇌졸중학회 · 대한뇌졸중영문학회지2006 · 제8권 제1호국소 뇌허혈에서 뇌저온 요법이 허혈 손상 및 Matrix Metalloproteinase 활성도에미치는 영향김경환, 김은희, 이경열, 이병인, 허지회, 선우일남대한신경과학회 · 대한신경과학회지2003 · 제21권 제4호선택적 Cyclooxygenase-2 억제재인 Rofecoxib가 쥐의 해마에서 만성 저혈류로 유발된 아포으토시스에 미치는 효과김범생, 김영인, 박상희, 이광수, 이원선, 이희진, 정성우, 최영빈대한신경과학회 · 대한신경과학회지2002 · 제20권 제6호Nicotinamide의 투여가 일시적인 국소허혈로 유발된 흰쥐의 뇌경색에 미치는 영향강구, 강성식, 김근우, 안영준, 이건재, 이민섭, 정일영, 최기용대한병리학회 · Journal of Pathology and Translational Medicine2006 · 제40권 제2호

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