Estradiol Valerate에 의해 유도된 다낭성난소와 CHO세포에서 NGF발현
Expression of NGF in Estradiol Valerate-Induced Polycystic Ovary and CHO Cells
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Polycystic ovary syndrome (PCOS) is hormonal imbalance condition as the endocrine and metabolic disorder that induces the infertility and various complications in reproductive age women. Estradiol valerate (EV) is used hormone replacement therapy in menopausal women and is reported that excessive administration of EV induces the PCOS. Nerve growth factor (NGF) is the factor to regulate the survival and maturation of developing neuronal cell and is also synthesized in ovary. And NGF is overexpressed in EV-induced polycystic ovary (PCO) as previously reported. Therefore, this study examined the possibility of NGF as can be used the biological marker in diagnosis of PCOS, the hormonal imbalance condition,using PCO and CHO (chinese hamster ovarian) cell lines. The concentration of EV treatment is optimized a 1 mg as not influence on the proliferation of CHO cell but 2 mg and 3 mg of EV treatment have the inhibition effect at initial stage. The morphological change was not observed in CHO cell after dose dependent manner treatment of EV. Expression of NGF mRNA and protein is significantly increased at 30 min after EV treatment in CHO cells compared to that of control. And NGF protein expression is strongly increased in PCO tissue, which observed many follicular cysts compared to normal ovary tissue. Taken together, overexpression of NGF may be act as a molecule to induce an abnormal development of follicle, suggesting that NGF can be used as a biological marker in diagnosis of PCOS.
Abstract
Polycystic ovary syndrome (PCOS) is hormonal imbalance condition as the endocrine and metabolic disorder that induces the infertility and various complications in reproductive age women. Estradiol valerate (EV) is used hormone replacement therapy in menopausal women and is reported that excessive administration of EV induces the PCOS. Nerve growth factor (NGF) is the factor to regulate the survival and maturation of developing neuronal cell and is also synthesized in ovary. And NGF is overexpressed in EV-induced polycystic ovary (PCO) as previously reported. Therefore, this study examined the possibility of NGF as can be used the biological marker in diagnosis of PCOS, the hormonal imbalance condition,using PCO and CHO (chinese hamster ovarian) cell lines. The concentration of EV treatment is optimized a 1 mg as not influence on the proliferation of CHO cell but 2 mg and 3 mg of EV treatment have the inhibition effect at initial stage. The morphological change was not observed in CHO cell after dose dependent manner treatment of EV. Expression of NGF mRNA and protein is significantly increased at 30 min after EV treatment in CHO cells compared to that of control. And NGF protein expression is strongly increased in PCO tissue, which observed many follicular cysts compared to normal ovary tissue. Taken together, overexpression of NGF may be act as a molecule to induce an abnormal development of follicle, suggesting that NGF can be used as a biological marker in diagnosis of PCOS.
참고문헌 (32)
- Amato G, "Serum and follicular fluid cytokines in polycystic ovary syndrome during stimulated cycles", Obstet Gynecol, 101, pp.1177~1182 (2003)
- Artini PG, "Growth factors and folliculogenesis in polycystic ovary patients", Expert Rev Endocrinol Metab, 2, pp.215~223 (2007)
- Balen AH, "The pathogenesis of polycystic ovary syndrome: the enigma unravels", Lancet, 354, pp.966~967 (1999)
- Barria A, "Ovarian steroidal response to gonadotropins and beta-adrenergic stimulation is enhanced in polycystic ovary syndrome: role of sympathetic innervation", Endocrinology, 133, pp.2696~2703 (1993)
- Conner P, "Expression of p53 and markers for apoptosis in breast tissue during long-term hormone therapy in cynomolgus monkeys", Am J Obstet Gynecol, 193(1), pp.58~63 (2005)
- Creasy GW, "Review of the endometrial effects of estrogens and progestins", Obstet Gynecol Surv, 47(9), pp.654~678 (1992)
- Dissen GA, "Excessive ovarian production of nerve growth factor facilitates development of cystic ovarian morphology in mice and is a feature of polycystic ovarian syndrome in humans", Endocrinology, 150(6), pp.2906~2914 (2009)
- Dissen GA, "A role for trkA nerve growth factor receptors in mammalian ovulation", Endocrinology, 137, pp.198~209 (1996)
- Dissen GA, "Intraovarian excess of nerve growth factor increases androgen secretion and disrupts estrous cyclicity in the rat", Endocrinology, 141(3), pp.1073~1082 (2000)
- Do Y, "Role of death receptor pathway in estradiol-induced T-cell apoptosis in vivo", Toxicol Sci, 70(1), pp.63~72 (2002)
- Dören M, "Basic principles of hormone replacement therapy in the postmenopause", Ther Umsch, 57(10), pp.628~634 (2000)
- Ferin M, "Menstruaˇcní cyklus", pp.80~83 (1997), Grada Publishing
- Franks S, "Polycystic ovary syndrome", N Engl J Med, 333, pp.853~861 (1995)
- Lara HE, "An increased intraovarian synthesis of nerve growth factor and its low affinity receptor is a principal component of steroid-induced polycystic ovary in the rat", Endocrinology, 141, pp.1059~1072 (2000)
- Lara HE, "Activation of ovarian sympathetic nerves in polycystic ovary syndrome", Endocrinology, 133(6), pp.2090~2695 (1993)
- Lara HE, "Involvement of nerve growth factor in female sexual development", Endocrinology, 126, pp.364~375 (1990)
- Li HJ, "P2Y(2) receptor-mediated modulation of estrogen-induced proliferation of breast cancer cells", Mol Cell Endocrinol, 338(1), pp.28~37 (2011)
- Sok Cheon Pak, "Effect of Korean Red Ginseng Extract in a Steroid-induced Polycystic Ovary Murine Model", Archives of Pharmacal Research, 32(3), pp.347~352 (2009), 대한약학회
- Proto MC, "Interaction of endocannabinoid system and steroid hormones in the control of colon cancer cell growth", J Cell Physiol (2011)[DOI]
- Seidel MF, "Nerve growth factor in rheumatic diseases", Semin Arthritis Rheum, 40(2), pp.109~126 (2010)
- Shirwalkar H, "Exposure of adult rats to estradiol valerate induces ovarian cyst with early senescence of follicles", Mol Cell Endocrinol, 272(1), pp.22~37 (2007)
- Snider WD, "Functions of the neurotrophins during nervous system development: what the knockouts are teaching us", Cell, 77(5), pp.627~638 (1994)
- Sofroniew MV, "Nerve growth factor signaling, neuroprotection, and neural repair", Annu Rev Neurosci, 24, pp.1217~1281 (2001)
- Stener-Victorin E, "Steroid-induced polyystic ovaries in rats: effect of electro-acupuncture on concentrations of endothelin-1 and nerve growth factor (NGF), and expression of NGF mRNA in the ovaries, the adrenal glands, and the centralnervous system", Reprod Biol Endocrinol, 1, pp.33~40 (2003)
- Taishi P, "Cytokine mRNA induction by interleukin-1beta or tumor necrosis factor alpha in vitro and in vivo", Brain Res, 1226, pp.89~98 (2008)
- Takaoka K, "Inflammatory cytokine tumor necrosis factor-alpha enhances nerve growth factor production in human keratinocytes, HaCaT cells", J Pharmacol Sci, 111(4), pp.381~391 (2009)
- Taylor AE, "Determinants of abnormal gonadotropin secretion in clinically defined women with polycystic ovary syndrome", J Clin Endocrinol Metab, 82, pp.2248~2256 (1997)
- Tokusashi Y, "Expression of NGF in hepatocellular carcinoma cells with its receptors in non-tumor cell components", Int J Cancer, 114, pp.39~45 (2005)
- Tokushige N, "High density of small nerve fibres in the functional layer of the endometrium in women with endometriosis", Hum Reprod, 21, pp.782~787 (2006)
- Toyoda M, "Nerve growth factor and substance P are useful plasma markers of disease activity in atopic dermatitis", Br J Dermatol, 147(1), pp.71~79 (2002)
- Vaitukaitis JL, "Polycystic-ovary syndrome-what is it", N Engl J Med, 309, pp.1245~1246 (1983)
- Zhuang YH, "Cell type-specific induction of cyclin D and cyclin-dependent kinase inhibitor p27(kip1) expression by estrogen in rat endometrium", J Steroid Biochem Mol Biol, 78(2), pp.193~199 (2001)