Biologic Response to Carbonated Hydroxyapatite Associated with Orthopedic Device: Experimental Study in a Rabbit Model
2
조회
0
다운로드
0
인용
0
참고문헌
키워드
초록
Background: Carbonated hydroxyapatite (CHA) and related calcium phosphates have been studied for many years as implant materials due to their similarity with the mineral phase of bone. The main limitation of CHA ceramics as well as other bioactive materials is that they have poor mechanical proprieties. It is thought that the mechanical device can cause an increase in metabolic activity and bone healing. In this study we investigated the reactivity and tissue behaviour of implanted CHA biomaterial reinforced by mini external fixator. Methods: The evaluation of biomaterial biocompatibility and osteogenesis was performed on a rabbit model over a period of 6 weeks by radiological, histological and scanning electron microscopy (SEM) coupled with energy dispersive X-ray SEM-energy-dispersive X-ray (EDX) analysis. Results: While rabbits treated with CHA exhibited more bone formation, and fibrous tissue was observed when empty bone defects were observed. EDX analysis detected little calcium and phosphorus on the surface of the bone that was not implanted, while high content of calcium (62.7%) and phosphorus (38%) was found on the interface bone cement. Conclusions: Bone repairing showed that the mini external fixator stimulated the ossification which was pushed when grafted by CHA. This effect may play an important role in the prevention of implant loosening.
Abstract
Background: Carbonated hydroxyapatite (CHA) and related calcium phosphates have been studied for many years as implant materials due to their similarity with the mineral phase of bone. The main limitation of CHA ceramics as well as other bioactive materials is that they have poor mechanical proprieties. It is thought that the mechanical device can cause an increase in metabolic activity and bone healing. In this study we investigated the reactivity and tissue behaviour of implanted CHA biomaterial reinforced by mini external fixator. Methods: The evaluation of biomaterial biocompatibility and osteogenesis was performed on a rabbit model over a period of 6 weeks by radiological, histological and scanning electron microscopy (SEM) coupled with energy dispersive X-ray SEM-energy-dispersive X-ray (EDX) analysis. Results: While rabbits treated with CHA exhibited more bone formation, and fibrous tissue was observed when empty bone defects were observed. EDX analysis detected little calcium and phosphorus on the surface of the bone that was not implanted, while high content of calcium (62.7%) and phosphorus (38%) was found on the interface bone cement. Conclusions: Bone repairing showed that the mini external fixator stimulated the ossification which was pushed when grafted by CHA. This effect may play an important role in the prevention of implant loosening.
참고문헌 (19)
- Huh GY, "Extraskeletal osteosarcoma: a case report", Korean J Pathol, 22, pp.489~494 (1988)
- Kotoura Y, "Application of biomaterials in the treatment of osteosarcoma", Gan To Kagaku Ryoho, 14(5 Pt 2), pp.1423~1429 (1987)
- Friedlaender GE, "Immune responses to osteochondral allografts: nature and significance", Orthopedics, 15, pp.1171~1175 (1992)
- Einhorn TA, "The healing of segmental bone defects induced by demineralized bone matrix: a radiographic and biomechanical study", J Bone Joint Surg Am, 66, pp.274~279 (1984)
- Tsukeoka T, "Enhanced fixation of implants by bone ingrowth to titanium fiber mesh: effect of incorporation of hydroxyapatite powder", J Biomed Mater Res B Appl Biomater, 75, pp.168~176 (2005)
- Tieanboon P, "Efficacy of hydroxyapatite in pedicular screw fixation in canine spinal vertebra", Asian Biomed, 3, pp.177~181 (2009)
- Miño-Fariña N, "Quantitative analysis of the resorption and osteoconduction of a macroporous calcium phosphate bone cement for the repair of a critical size defect in the femoral condyle", Vet J, 179, pp.264~272 (2009)
- Liljensten E, "Resorbable and nonresorbable hydroxyapatite granules as bone graft substitutes in rabbit cortical defects", Clin Implant Dent Relat Res, 5, pp.95~101 (2003)
- Badraoui R, "Effect of subchronic exposure to tetradifon on bone remodelling and metabolism in female rat", C R Biol, 330, pp.897~904 (2007)
- Doi Y, "Sintered carbonate apatites as bioresorbable bone substitutes", J Biomed Mater Res, 39, pp.603~610 (1998)
- Landi E, "Carbonated hydroxyapatite as bone substitute", J Eur Ceram Soc, 23, pp.2931~2937 (2003)
- Pan H, "Apatite-formation ability: predictor of “bioactivity”?", Acta Biomater, 6, pp.4181~4188 (2010)
- Okumura M, "Osteoblastic phenotype expression on the surface of hydroxyapatite ceramics", J Biomed Mater Res, 37, pp.122~129 (1997)
- Mastrogiacomo M, "Role of scaffold internal structure on in vivo bone formation in macroporous calcium phosphate bioceramics", Biomaterials, 27, pp.3230~3237 (2006)
- Rey C, "Fourier transform infrared spectroscopic study of the carbonate ions in bone mineral during aging", Calcif Tissue Int, 49, pp.251~258 (1991)
- Ooms EM, "Trabecular bone response to injectable calcium phosphate (Ca-P) cement", J Biomed Mater Res, 61, pp.9~18 (2002)
- Yuan H, "Tissue responses of calcium phosphate cement: a study in dogs", Biomaterials, 21, pp.1283~1290 (2000)
- Theiss F, "Biocompatibility and resorption of a brushite calcium phosphate cement", Biomaterials, 26, pp.4383~4394 (2005)
- Eriksson C, "Callus formation and remodeling at titanium implants", J Biomed Mater Res A, 83, pp.1062~1069 (2007)