본문 바로가기
KERD
논문KCI 등재

지방기질유래 줄기세포의 골 분화 시 성장인자의 효과

The effect of growth factors on osteogenic differentiation of adipose tissue-derived stromal cells

최연식|정진섭|김욱규
대한구강악안면외과학회 (Korean Association Of Oral And Maxillofacial Surgeons)|2006.08|대한구강악안면외과학회지 (Journal of the Korean Association of Oral and Maxillofacial Surgeons)|제32권 제4호|pp. 327-333

2

조회

0

다운로드

1

인용

0

참고문헌

주제분류치의학

초록

Abstract

Future cell-based therapies such as tissue engineering will benefit from a source of autogenous pluripotent stem cells. There are embryonic stem cells (ESC) and autologous adult stem cells, two general types of stem cells potentilally useful for these applications. But practical use of ESC is limited due to potential problems of cell regulation and ethical considerations. To get bone marrow stem cells is relatively burden to patients because of pain, anesthesia requirement. The ideal stem cells are required of such as the following advantages: easy to obtain, minimal patient discomfort and a capability of yielding enough cell numbers. Adipose autologus tissue taken from intraoral fatty pad or abdomen may represent such a source. Our study designed to demonstrate the ability of human adipose tissue-derived stromal cells (hATSC) from human abdominal adipose tissue diffentiating into osteocyte and adipocyte under culture in vitro conditions. As a result of experiment, we identified stromal cell derived adipose tissue has the multilineage potentiality under appropriate culture conditions. And the adipose stromal cells expressed several mesenchymal stem cell related antigen (CD29, CD44) reactions. Secondary, we compared the culture results of a group of hATSC stimulated with TGF-β1, bFGF with a hATSC group without growth factors to confirm whether cytokines have a important role of the proliferation in osteogenic differentiation. The role of cytokines such as TGF-β1, bFGF increased hATSC’s osteogenic differentiation especially when TGF-β1 and bFGF were used together. These results suggest that adipose stromal cells with growth factors could be efficiently available for cell-based bone regeneration.

참고문헌 (31)

  1. "Heterotopic of bone marrow.Analysis of precursor cells for osteogenic and hematopoietic tissues", 6, pp.230~247 (1968)
  2. "Stromal cell-associated hematopoiesis", 1, pp.87~94 (1982)
  3. "Marrow stromal stem cells", 10, pp.63~76 (1988)
  4. "Growth kinetics,self-renewal,and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation", 64, pp.278~294 (1997)
  5. "Multilineage potential of adult haman mesenchymal stem cells", 284, pp.143~147 (1999)
  6. "Histology of the stroma in developing rat subcutaneous adipose tissue", 55, pp.1336~1343 (1982)
  7. "Cells in human adipose tissue developing into adipocytes", 215, pp.447~451 (1984)
  8. "Adipocyte presursor cells in obese and nonobese humans", 34, pp.808~812 (1985)
  9. "Multilineage cells from human adipose tissue implications for cell-based therapies", 7, pp.211~228 (2001)
  10. "Extracellular matrix mineralization and osteoblast gene expression by human adipose tissue-derived stromal cells", 7(6), pp.729~741 (2001)
  11. "Mesenchymal stem cells", 9, pp.641~650 (1991)
  12. "In vitro chondrogenesis of bone marrow derived mesenchymal progenitor cells", 238, pp.265~272 (1998)
  13. "Myogenic cells derived from rat bone marrow mesenchymal stem cells exposed to 5-azacytidine", 18, pp.1417~1426 (1995)
  14. "Muscle regeneration by bone marrow-derived myogenic progenitors", 279, pp.1528~1530 (1998)
  15. "Marrow stromal cells as stem cells for non-hematopoietic tissues", 276, pp.71~74 (1997)
  16. "Dystrophin expression in the mdx mouse restored by stem cell transplantation", 401, pp.390~394 (1999)
  17. "Neovascularization of ischemic myocardium by human bone marrow-derived angiblasts prevents cardiomyocyte apoptosis,reduces remodeling and improves cardiac function", 7, pp.430~436 (2001)
  18. "Bone marrow cells regenerate infarcted myocardium", 410, pp.701~705 (2001)
  19. "Hepatocytes from non-hepatic adult stem cells", 406, pp.257~ (2000)
  20. "Purified hematopoietic stem cells can differentiate into hepatocytes in vivo", 6, pp.1229~1234 (2000)
  21. "Liver from bone marrow in humans", 32, pp.11~16 (2000)
  22. "From marrow to brain:expression of neuronal phenotypes in adult mice", 290, pp.1775~1779 (2000)
  23. "Turning blood into brain:cells bearing neuronal antigens generated in vivo from bone marrow", 290, pp.1770~1782 (2000)
  24. "Isolation of multilineage mesenchymal stem cells from human adipose tissue and proximal femur" (2002), Pusan National University, College of Medicine, Postgraduate School
  25. "In vitro effects of growth factors and dexamethasone on rat marrow stromal cell", 313 (1995)
  26. "Responses to growth factors", pp.32~7
  27. "Osteogenic Potential of Cultured Bone Marrow Stromal Cells Transfected with Transforming Growth Factor β1 Gene in vitro", 21(2), pp.130~133 (2001)
  28. "Effect of different growth factors on the chondrogenic potential of human bone marrow stromal cells", 9, pp.36~40 (2001)
  29. "A Recombinant Human TGF-β1 Fusion Protein with Collagen-binding Domain Promotes Migration,Growth,and Differentiation of Bone Marrow Mesenchymal Cells", 250, pp.485~498 (1999)
  30. "Effect of Maturation on the Osteogenic Response of Cultured Stromal Bone Marrow Cells to Basic Fibroblast Growth Factor", 27(6), pp.777~783 (2000)
  31. "Retention of Multilineage Differentiation Potential of Mesenchymal Cells during Proliferation in Response to FGF", 288, pp.413~419 (2001)

관련 논문AI추천

bFGF, PDGF-BB가 백서 골수기원 간엽 줄기세포의 조직골세포 분화에 미치는 영향에 관한 연구송진아, 최진영대한구강악안면외과학회 · 대한구강악안면외과학회지2006 · 제32권 제6호협부지방에서 성체 줄기세포의 분리와 골모 세포로의 분화김창현, 박장우, 이일규, 표성운대한구강악안면외과학회 · 대한구강악안면외과학회지2006 · 제32권 제6호인간 지방조직에서 분리된 줄기세포의 표면항원 및 다분화능 확인조혜경대한임상검사과학회 · 대한임상검사과학회지2008 · 제40권 제2호이형상 칼슘 인산염(Biphasic Calcium Phosphate)에 의한 성체 줄기세포의 골아세포 분화김윤상, 신형식, 유형근, 이명구, 임성우, 피성희대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제4호지방줄기세포를 이용한 골재생이재선, 임군일대한골대사학회 · 대한골대사학회지2011 · 제18권 제1호개의 지방조직내 중배엽줄기세포를 이용한 연골세포의 분화서철주, 왕수건, 이량화, 이병주, 이진춘, 정진섭대한이비인후과학회 · 대한이비인후과학회지 두경부외과학2004 · 제47권 제8호

이 간행물의 다른 논문 (12건)

구순열비변형 환자에서 비교정술에 대한 비교 연구유선열pp. 287-294비관형 천연 셀룰로오스막 도관을 이용한 말초신경 재생에 대한 실험적 연구김성민, 이석근, 이종호pp. 295-307안면신경 압박손상 후 Adenovirus 매개 BDNF 유전자 전달을 통한 신경손상 회복에 관한 연구양병은, 이종호pp. 308-316성견의 골신장술에서 골절단술시 재조합 인간 골형성 단백질-7적용에 따른 가골반응과Osteocalcin 발현도에 대한 연구변준호, 김욱규, 성일용, 박봉욱, 조영철, 신상훈, 김종렬pp. 317-326구강악안면외과 의료행위 상대가치 개발에 대한 조사연구송진아, 유순용, 황종민, 백경원, 최진영pp. 334-347
원문 보기