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협부지방에서 성체 줄기세포의 분리와 골모 세포로의 분화

Differentiation of adult stem cell derived from buccal fat pad into osteoblast

김창현|박장우|이일규|표성운
대한구강악안면외과학회 (Korean Association Of Oral And Maxillofacial Surgeons)|2006.12|대한구강악안면외과학회지 (Journal of the Korean Association of Oral and Maxillofacial Surgeons)|제32권 제6호|pp. 524-529

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참고문헌

주제분류치의학

초록

Abstract

For the repairing of bone defect, autogenous or allogenic bone grafting remains the standard. However, these methods have numerous disadvantages including limited amount, donor site morbidity and spread of diseases. Tissue engineering technique by culturing stem cells may allow for a smart solution for this problem. Adipose tissue contains mesenchymal stem cells that can be differentiate into bone, cartilage, fat or muscle by exposing them to specific growth conditions. In this study, the authors procured the stem cell from buccal fat pad and differentiate them into osteoblast and are to examine the bone induction capacity. Buccal fat-derived cells (BFDC) were obtained from human buccal fat pad and cultured. BFDC were analyzed for presence of stem cell by immunofluorescent staining against CD-34, CD-105 and STRO-1. After BFDC were differentiated in osteogenic medium for three passages, their ability to differentiate into osteogenic pathway were checked by alkaline phosphatase (ALP) staining, Alizarin red staining and RT-PCR for osteocalcin (OC) gene expression. Immunofluorescent and biochemical assays demonstrated that BFDC might be a distinguished stem cells and mineralization was accompanied by increased activity or expression of ALP and OC. And calcium phosphate deposition was also detected in their extracelluar matrix. The current study supports the presence of stem cells within the buccal fat pad and the potential implications for human bone tissue engineering for maxillofacial reconstruction.

참고문헌 (25)

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관련 논문AI추천

지방기질유래 줄기세포의 골 분화 시 성장인자의 효과김욱규, 정진섭, 최연식대한구강악안면외과학회 · 대한구강악안면외과학회지2006 · 제32권 제4호BMP-2에 의한 협부 지방 성체 줄기세포의 골형성김창현, 박철헌, 이일규, 표성운대한구강악안면외과학회 · 대한구강악안면외과학회지2008 · 제34권 제4호인간 지방조직에서 분리된 줄기세포의 표면항원 및 다분화능 확인조혜경대한임상검사과학회 · 대한임상검사과학회지2008 · 제40권 제2호이형상 칼슘 인산염(Biphasic Calcium Phosphate)에 의한 성체 줄기세포의 골아세포 분화김윤상, 신형식, 유형근, 이명구, 임성우, 피성희대한치주과학회 · Journal of Periodontal & Implant Science2005 · 제35권 제4호배양 치조골모세포의 생물학적 특성에 관한 연구김성윤, 김현아, 신계철, 신형식, 유석주, 유형근, 이성진, 이용배대한치주과학회 · Journal of Periodontal & Implant Science2004 · 제34권 제2호지방줄기세포를 이용한 골재생이재선, 임군일대한골대사학회 · 대한골대사학회지2011 · 제18권 제1호

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