개의 지방조직내 중배엽줄기세포를 이용한 연골세포의 분화
Chondrogenesis of Mesenchymal Stem Cell Derived from Canine Adipose Tissue
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Abstract
Background and Objectives：Tisue enginering is presently being utilized in parts of cartilage repair. Sources of cells for tissue enginering are chondrocytes from mature cartilage and bone marrow mesenchymal stem cells that are able to diferentiate into chondrocytes. Recent studies have shown that adipose tisue have mesenchymal stem cels which can diferentiate into adipogenic, chondrogenic myogenic, osteogenic cels and neural cels in vitro. In this study, we have examined chondrogenic potential of the canine adipose tisue-derived mesenchymal stem cell (ATSC). Materials and Method：We harvested canine adipose tisue from the inguinal area. ATSCs were enzymatically released from canine adipose tissue. Under apropriate culture conditions, ATSCs were induced to diferentiate into chondrocyte lineages using micromass culture technique. We used imunostain to type I colagen and toluidine blue stain to confirm chondrogenic diferentiation of ATSCs. Results：We could isolate ATSCs from canine adipose tisue. ATSCs that has positive response to immunostain of type I collagen and toluidine blue stain. Conclusion：In vitro, ATSCs diferentiated into cels that have characteristic cartilage matrix molecules in the presence of lineage-specific induction factors. Adipose tissue may represent an alternative source to bone marow-derived MSCs.
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