젊은 여성들에서 고강도 운동 후 혈중 산화스트레스 지표들의 변화
Changes of Blood Oxidative Stress Markers after a High-Intensity Exercise in Young Women
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연구배경 적절한 강도를 벗어난 급격한 운동은 산화스트레스를 유발할 가능성이 높은 것으로 보고되고 있다. 따라서, 본 연구는 젊은 여성들에서 고강도운동 후 혈중 산화스트레스 지표들의 변화에 대하여 조사하는데 목적을 두었다. 방 법 운동 습관이 없고, 규칙적인 월경주기를 지닌 8명의 일반여성들이 본 연구에 참가하였다. 2개월간의 기초체온 측정과 뇨에 의한 배란 검사를 실시하여, 규칙적인 월경주기를 지닌 것으로 확인되었다. 모든 피험자는 고강도운동(80% VO2max 강도, 22.5minute)을 실시하였다. 젖산은 운동 전(Pre)과 운동 직후(PO)에 채혈된 혈액에서 측정되었다. 혈중 산화스트레스 지표 (적 혈구 TBARS, SOD, GPx 그리고 혈청 CPK)들은 Pre, PO, P2, P12 및 P24에서 채혈된 혈액에서 측정되었다. 결 과 혈중 젖산은 Pre와 비교하여 P0에서 유의하게 증가하였다. 적혈구 TBARS는 Pre와 비교하여 P12에서 유의하게 증가하였다. 적혈구 SOD 활성은 Pre와 비교하여 P24에서 유의하게 감소하였다. 적혈구 GPx활성은 Pre와 비교하여 운동 후 시간경과에 따른 유의한 변화를 나타내지 않았다. 혈청 CPK는 Pre와 비교하여 P0에서는 유의하게 감소하였으며, P12에서는 유의하게 증 가하였다. 결 론 규칙적인 월경주기를 지닌 일반여성에서 월경기에 실시한 고강도운동은 혈중 산화스트레스를 증가시키며, 특히 고강도 운동을 마친 뒤 12시간 이후에는 활성산소의 영향이 가장 큰 것으로 나타났다. 그러므로 운동습관이 없는 일반여성은 월경기에 강도가 높은 격렬한 운동을 피하는 것이 바람직할 것으로 사료된다.
Abstract
Background It is reported that high-intensity physical exercise may induce oxidative stress. Therefore, the purpose of this study was to investigate the change of blood oxidative stress markers after a high-intensity exercise in young women Methods Eight sedentary women who have regular menstrual cycles participated in this study. Regular menstrual cycles were determined by basal body temperature (BBT) and urinary ovulation test for 2 mouths prior to this study. All subjects performed the bicycle ergometer exercise (for 22.5 min at 80% VO2max). Lactate was determined from blood of Pre-and Post-exercise (PO). Blood oxidative stress markers (erythrocyte thiobarbituric acid reactive substances: TBARS, Superoxide dismutase: SOD, Glutathione Peroxidase: GPx and serum Creatine PhosphoKinase: CPK) were determined from blood of Pre- and Post-exercise (PO, P2, P12 and P24). Results In this results, lactate was increased clearly at the P0 compared with Pre (p<0.05). And TBARS increased significantly P12 compared with Pre (p<0.05). SOD activity also decreased significantly P24 compared with Pre (p<0.05). However, GPx activity did not show statistically significant changes by the high-intensity exercise. CPK significantly decreased P0 and increased significantly P12 compared with Pre (p<0.05). Conclusions In the menses, high-intensity exercise may increase the blood oxidative stress in the young woman which has a normal menstrual cycle. In particular, the influence of ROS is very high after P12. Therefore, sedentary women had better do not intense exercise in the menses.
참고문헌 (29)
- Durstine JL, "Effects of exercise training on plasma lipids and lipoproteins", Exer Sport Sci Rev, 22, pp.477~521 (1994)
- Ginsburg GS, "Gender differences in exercise-induced changes in sex hormone levels and lipid peroxidation in athletes participating in the Hawaii Ironman triathlon. Ginsburg-gender and exercise-induced lipid peroxidation", Clin Chim Acta, 305, pp.131~139 (2001)
- Urso ML, "Oxidative stress, exercise and antioxidant supplementation", Toxicology, 189, pp.41~54 (2003)
- Finkel T, "Oxidants, oxidative stress and the biology of ageing", Nature, 408, pp.239~247 (2000)
- Cao GH, "Effects of dietary zinc on free radical generation, lipid peroxidation, and superoxide dismutase in trained mice", Arch Biochem Biophys, 291, pp.147~153 (1991)
- Lawler JM, "Oxidative stress, antioxidant status, and the contracting diaphragm", Can J Appl Physiol, 23, pp.23~55 (1998)
- Ji LL, "Exercise and oxidative stress: role of the cellular antioxidant systems", Exerc Sport Sci Rev, 23, pp.135~166 (1995)
- Laaksonen DE, "Increased resting and exercise-induced oxidative stress in young IDDM men", Diabets Care, 19, pp.569~574 (1996)
- Toshinai K, "Effect of Different Intensity and Duration of Exercise with the Same Total Oxygen Uptakeon Lipid Peroxidation and Antioxidant Enzyme Levels in Human Plasma", Adv Exerc Sports Physiol, 4, pp.65~70 (1998)
- Ayres S, "Mechanisms involved in the protective effect of estradiol-17β on lipid peroxidation and DNA damage", Am J Physiol, 274, pp.E1002~E1008 (1998)
- Joo MH, "The relationship between exercise-induced oxidative stress and menstrual cycle", Eur J Appl Physiol, 93, pp.82~86 (2004)
- Braun D, "Fluorescence interference-contrast microscopy of cell adhesion on oxidized silicon", Appl Phys A, 65, pp.341~348 (1997)
- McCord JM, "Superoxide dismutase: An enzymic function for erythrocuprein (hemocuprein)", J Biol Chem, 244, pp.6049~6055 (1969)
- 金澤健一, "電気化学連続発光法の原理及びその応用", 医学と薬学, 41, pp.718~724 (1999)
- Dillard DB, "Caluculation of percentage change in volumes of blood，plasma，and red cells in dehydration", J Appl Physiol, 137, pp.247~248 (1978)
- 이복환, "운동강도에 따른 지질 과산화물과체내 항산화효소의 활성정도와 항산화제의 방어효과", 한국체육학회지, 40, pp.661~674 (2001)
- Sumikawa K, "Changes in erythrocyte membrane phospholipids composition induced by physical training and physical exercise", Eur j Appl Physiol, 67, pp.132~137 (1993)
- Senturk UK, "Exercise-induced oxidative stress leads hemolysis in sedentary but not trained humans", J Appl Physiol, 99, pp.1434~1441 (2005)
- Miyazaki H, "Strenuous endurance training in humans reduces oxidative stress following exhausting exercise", Eur J Appl Physiol, 84, pp.1~6 (2001)
- Yalcin O, "Effect of swimming exercise on red blood cell theology in trained and untrained rats", J Appl Physiol, 88, pp.2074~2080 (2000)
- Tiidus PM, "Radical species in inflammation and overtraining", Can J Physiol Pharmacol, 76, pp.533~538 (1998)
- Oztasan N, "Endurance training attenuates exercise-induced oxidative stress in erythrocytes in rat", Eur J Appl Physiol, 91, pp.622~627 (2004)
- Ohno H, "Training effects on concentration ofimmunoreative superoxide dismutase isoenzymes in human plasma", Tohoku J. of Experimental Medicine, 167, pp.301~303 (1992)
- 진영수, "운동양식과 항산화비타민의 보충이 항산화효소 및 총 항산화능에 미치는 영향", 한국체육학회지, 39, pp.451~460 (1999)
- Itoh H, "Vitamin E supplementation attenuatesleakage of enzymes following 6 successive days of running training", Int J Sports Med, 21, pp.369~374 (2000)
- Hunter JB, "Effect of training on plasma enzyme levels in man", J Appl Physiol, 31, pp.20~23 (1971)
- Lijnen P, "Erythrocyte 2, 3-diphosphoglycerate and serum enzyme concentrations in trained and sedentary men", Med Sci Sports Exerc, 18, pp.174~179 (1986)
- 현송자, "운동생화학" (1990), 보경문화
- 伊藤朗, "図説․運動生化学入門 -生理生化学から運動処方まで-" (1994), 医歯薬出版