다른 월경주기상태를 지닌 여자 운동선수들에서 급성운동이 혈중 산화스트레스 지표에 미치는 영향
Influence of Acute Exercise on Blood Oxidative Stress Markers in Female Athletes with the Different Menstrual Cycle Status
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연구배경 격렬한 신체활동은 인체내에서 자유라디칼과 활성산소종의 생산을 촉진시킬 가능성이 있는것으로 알려져 있다. 따라서, 본 연구는 다른 월경주기 상태를 지닌 젊은 여자 운동선수들에서 급성운동이 혈중 산화스트레스 지표에 미치는 영향을 조사하는데 목적을 두었다. 방 법 총16명의 여자 운동선수들을 대상으로, 2개월간 기초 체온 측정과 배란 검사를 실시한뒤, 정상 월경군(EA, n=8)과 월경이상군(IMA, n=8)으로 분류하여, 자전거 에르고미터를 이용한 급성운동을 실시하였다. 운동전·후에 채혈한 혈액샘플에서estradiol과 산화스트레스 지표인 TBARS (thiobarbituric acid reactive substances), T-SOD (total superoxide dismutase) 활성, CPK (creatine phosphokinase), glutathione 산화-환원 상태를 나타내는 지표들(TGSH, GSSG, GSH, GSSG-TGSH ratio)을 측정하였다. 결 과 초경시작연령은 월경이상군에서 늦은 경향을 나타내었으며, estradiol 농도는 월경이상군에서 유의하게 낮았다. TBARS는 정상월경군에서 급성운동 후에 증가되는 경향을 보였으나, 통계적으로 유의하지 않았다. T-SOD 활성은 정상월경군에서 급성운동후 감소되는 경향을 보였고, 월경이상군에서는 유의하게 감소되었다. CPK는 2군 모두 급성운동 후에 통계적으로 유의한 변화를 나타내지 않았다. Glutathione 산화-환원 상태는 급성운동후, GSSG가 정상월경군에서증가되는 경향을 보였으며, 월경이상군에서는 유의하게 증가되었다. GSH는 정상월경군에서 감소되는 경향을 보였다. GSSG/TGSH 비율은 정상월경군에서 증가되는 경향을 보였고, 월경이상군에서는 유의하게 증가되었다. 결 론 다른 월경주기 상태를 지닌 젊은여자 운동선수들에서 급성운동이 혈중 산화스트레스에 영향을 미칠 가능성이 있으며, 특히, 월경이상군에서 산화스트레스가 클것으로 사료된다. 따라서 앞으로는 월경이상을 지닌 여자운동선수들의 산화스트레스 예방에 관련된 연구들이 활발히 이루어질 필요가 있다.
Abstract
Background Intense physical exercise may promote production of free radicals in the body that may cause various types of oxidative damage. The purpose of this study is to investigate the effect of acute exercise on blood oxidative stress markers in female athletes with the different menstrual cycle status. Methods Subjects were divided into two groups(eumenorrheic athletes; EA, irregular menstrual athletes; IMA), and they performed acute exercise by bicycle ergometer. Estradiol, TBARS, T-SOD activity, CPK, Glutathione redox status indices (TGSH, GSSG, GSH, GSSG-TGSH ratio) were determined from blood sample of the pre-and post-exercise. Results TBARS of two groups showed no statistically significant changes by acute exercise. T-SOD activity decreased significantly in the IMA group but it tended to decrease during post-exercise period in the EA group. CPK of two groups showed no statistically significant changes by acute exercise. In the glutathione redox status, GSSG increased significantly in the IMA group but it tended to increase during post-exercise period in the EA group. GSSG/TGSH ratio decreased significantly in the IMA group but it tended to decrease by acute exercise in the EA group. Conclusions Acute exercise may influence on blood oxidative stress markers in the female athletes with the different menstrual cycle status, especially, it seems that the oxidative stress is larger in the IMA group. In the future, therefore, the study about the oxidative stress prevention in the female athletes who have irregular menstruation will be needed.
참고문헌 (26)
- Mesaki N, "Menstrual cycle and sports, In: Sports Medicine inWomen" (1997), Japan, Tokyo, Bunnkoutou
- "Marshall LA. Clinical evaluation of amenorrhea in active andathletic women. Clin Sports Med 1994", pp.371~387
- Kamada T, "Yagi K. Serumlipoperoxide level in patients suffering from liver disease. ClinChim Acta 1977", pp.267~270
- Urso ML, "Oxidative stress, exercise andantioxidant supplementation", 189, pp.41~54 (2003)
- Lacort M, "Protective effect of estrogens and catecholestrogens againstperoxidative membrane damage in vitro", 30, pp.141~146 (1995)
- Ayres S, "Mechanisms involvedin the protective effect of estradiol-17β on lipid peroxidation andDNA damage", 274, pp.E1002~E1008 (1998)
- Arteaga E, "In vitro effect of estradiol, progesterone, testosterone, and ofcombined estradiol", progestins on low density lipoprotein (LDL)oxidation in postmenopausal women, 1998, pp.5, 16~23
- Bednarek-Tupikowska G, "Effectsof oestradiol and oestroprogestin on erythrocyte antioxidativeenzyme system activity in postmenopausal women", 64, pp.463~468 (2006)
- Ilhan N, "Variable effectsof exercise intensity on reduced glutathione, thiobarbituric acidreactive substance levels, and glucose concentration", 35, pp.294~300 (2004)
- Joo MH, "The relationship between exercise-induced oxidative stress andmenstrual cycle", 93, pp.82~86 (2004)
- Joo MH, "Influence of exercise on resting blood oxidative stress markersin young women with the different menstrual cycle status", 12, pp.23~28 (2006)
- "P.-O. Human physical fitness with special reference toage and sex", pp.307~335 (1965)
- Makino T, "Nisie H. Studies on alternation of serum estradiol andprogesterone in women measured by new coated tube kits.Clinical Endocrinology 1988", pp.975~979
- "Yagi K. Assay for lipid peroxides in animaltissues by thiobarbituric acid reaction. Anal Biochem 1979", pp.351~358
- "Griffith OW. Determination of glutathione and glutathionedisulfide using glutathione reductase and 2-vinlypyridine. AnnBiochem 1980", pp.207~212
- Dill DB, "Costill DL. Calculation of percentage changes involumes of blood and red cells in dehydration. J ApplPhysiol 1974", pp.247~248
- Ginsburg GS, "Genderdifferences in exercise-induced changes in sex hormone levelsand lipid peroxidation in athletes participating in the HawaiiIronman triathlon.Ginsburg-gender and exercise-induced lipidperoxidation", 305, pp.131~139 (2001)
- Miyazaki H, "Strenuous endurance training in humans reducesoxidative stress following exhausting exercise", 84, pp.1~6 (2001)
- Laaksonen DE, "Increased resting and exercise-induced oxidative stressin young IDDM men", 19, pp.569~574 (1996)
- Senturk UK, "Exercise-induced oxidative stress leads hemolysis in sedentarybut not trained humans", 99, pp.1434~1441 (2005)
- "Laloraya MM. Changes in the levelsof superoxide anion radical and superoxide dismutase duringthe estrous cycle of Rattus norvegicus and induction ofsuperoxide dismutase in rat ovary by lutropin. BiochemBiophys Res Commun 1988", pp.146~153
- Ji LL, "Lardy HA. Enzymatic downregulation withexercise in rat skeletal muscle. Arch Biochem Biophys 1988", pp.137~149
- "PosnikJ. Effects of graded treadmill exercise on the activity of bloodantioxidant enzymes lipid peroxides and nonenzymaticanti-oxidants in long-distance skiers. Biol Sport 1994", pp.217~226
- Frei B, "BrooksGA. Antioxidant status and indexes of oxidative stress duringconsecutive days of exercise. J Appl Physiol 1993", pp.566~572
- Sen CK, "effect of N-acetylcysteine supplementation.J Appl Physiol 1994", pp.2570~2577
- Bruunsgaard H, "Exercise-induced increase in seruminterleukin-6 in humans is related to muscle damage", 499, pp.833~841 (1997)