Induction of Regulatory Dendritic Cells by Lactobacillus paracasei L9 Prevents Allergic Sensitization to Bovine β-Lactoglobulin in Mice
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초록
Supplementation with probiotics can protect against the development of food allergies by modulating the host immune response; however, the mechanisms are not fully understood. The objective of this study was to investigate the allergy-reducing effects of regulatory dendritic cells (regDCs) induced by Lactobacillus paracasei L9 (L9) in β-lactoglobulin (BLG)- sensitized mice. The L9 supplement suppressed the aberrant balance of Th1/Th2 responses to BLG in mice, via upregulation of the CD4+CD25+Foxp3+Treg cell responses. The amount of CD4+CD25+Foxp3+Treg cells in mesenteric lymph nodes increased by 51.85%. Furthermore, administration of L9 significantly induced the expression of CD103 and reduced the maturation status of DCs in mesenteric lymph nodes, Peyer’s patches, and spleen. Bone marrow-derived dendritic cells (BM-DCs) were activated by L9 in vitro, with an approximate 1.31-fold and 19.57-fold increase in expression of CD103 in CD11c+DCs and the level of IL-10 production, respectively, while the expression of CD86 did not change significantly. These data demonstrate that L9 reduced the BLG allergic sensitization, likely through regDCs mediated active suppression.
Abstract
Supplementation with probiotics can protect against the development of food allergies by modulating the host immune response; however, the mechanisms are not fully understood. The objective of this study was to investigate the allergy-reducing effects of regulatory dendritic cells (regDCs) induced by Lactobacillus paracasei L9 (L9) in β-lactoglobulin (BLG)- sensitized mice. The L9 supplement suppressed the aberrant balance of Th1/Th2 responses to BLG in mice, via upregulation of the CD4+CD25+Foxp3+Treg cell responses. The amount of CD4+CD25+Foxp3+Treg cells in mesenteric lymph nodes increased by 51.85%. Furthermore, administration of L9 significantly induced the expression of CD103 and reduced the maturation status of DCs in mesenteric lymph nodes, Peyer’s patches, and spleen. Bone marrow-derived dendritic cells (BM-DCs) were activated by L9 in vitro, with an approximate 1.31-fold and 19.57-fold increase in expression of CD103 in CD11c+DCs and the level of IL-10 production, respectively, while the expression of CD86 did not change significantly. These data demonstrate that L9 reduced the BLG allergic sensitization, likely through regDCs mediated active suppression.
참고문헌 (38)
- Adel-Patient K, "Evaluation of a high IgEresponder mouse model of allergy to bovine β-lactoglobulin (BLG): development of sandwich immunoassays for total and allergen-specific IgE, IgG1 and IgG2a in BLG-sensitized mice", J. Immunol. Methods, 235, pp.21~32 (2000)
- Akdis M, "Therapeutic manipulation of immune tolerance in allergic disease", Nat. Rev. Drug Discov, 8, pp.645~660 (2009)
- Akdis M, "Tregulatory cells in allergy: novel concepts in the pathogenesis, prevention, and treatment of allergic diseases", J. Allergy Clin. Immun, 116, pp.961~968 (2005)
- Berni CR, "The potential therapeutic efficacy of Lactobacillus GG in children with food allergies", Pharmaceuticals, 5, pp.655~664 (2012)
- Braun FC, "Can farm milk consumption prevent allergic diseases?", Clin. Exp. Allergy, 41, pp.29~35 (2011)
- Canani RB, "Gut microbiota as potential therapeutic target for the treatment of cow’s milk allergy", Nutrients, 5, pp.651~662 (2013)
- Christensen HR, "Lactobacilli differentially modulate expression of cytokines and maturation surface markers in murine dendritic cells", J. Immunol, 168, pp.171~178 (2002)
- Coombes JL, "A functionally specialized population of mucosal CD103+ DCs induces Foxp3+regulatory T cells via a TGF-β– and retinoic acid–dependent mechanism", J. Exp. Med, 204, pp.1757~1764 (2007)
- Dehzad N, "Regulatory T cells more effectively suppress Th1-induced airway inflammation compared with Th2", J. Immunol, 186, pp.2238~2244 (2011)
- Elawadli I, "Differential effects of lactobacilli on activation and maturation of mouse dendritic cells", Benef. Microbes, 5, pp.323~334 (2014)
- Finamore A, "Lactobacillus rhamnosus GG and Bifidobacterium animalis MB5 induce intestinal but not systemic antigenspecific hyporesponsiveness in ovalbumin-immunized rats", J. Nutr, 142, pp.375~381 (2012)
- Frei R, "Microbiota and dietary interactions – an update to the hygiene hypothesis?", Allergy, 67, pp.451~461 (2012)
- Fujita S, "Regulatory dendritic cells protect against cutaneous chronic graft-versus-host disease mediated through CD4+CD25+ Foxp3+ regulatory T cells", Blood, 110, pp.3793~3803 (2007)
- Inamine A, "Sublingual administration of Lactobacillus paracasei KW3110 inhibits Th2-dependent allergic responses via upregulation of PD-L2 on dendritic cells", Clin. Immunol, 143, pp.170~179 (2012)
- Jaensson E, "Small intestinal CD103+ dendritic cells display unique functional properties that are conserved between mice and humans", J. Exp. Med, 205, pp.2139~2149 (2008)
- Jeong Y, "Clonorchis sinensis-derived total protein attenuates airway inflammation in murine asthma model by inducing regulatory T cells and modulating dendritic cell functions", Biochem. Biophys. Res. Commun, 407, pp.793~800 (2011)
- Jo J, "Role of cellular immunity in cow’s milk allergy: pathogenesis, tolerance induction, and beyond", Mediat. Inflamm, pp.1~9 (2014)
- Kandasamy M, "Lactobacillus rhamnosus GG secreting an antigen and interleukin-2translocates across the gastrointestinal tract and induces an antigen specific immune response", Microbiol. Immunol, 55, pp.704~714 (2011)
- Kanjarawi R, "Regulatory CD4+ Foxp3+ T cells control the severity of anaphylaxis", PLoS One, 8, pp.e69183~ (2013)
- Konieczna P, "Bifidobacterium infantis 35624administration induces Foxp3 T regulatory cells in human peripheral blood: potential role for myeloid and plasmacytoid dendritic cells", Gut, 61, pp.354~366 (2012)
- Kumar S, "Molecular mechanisms of IgE mediated food allergy", Int. Immunopharmacol, 13, pp.432~439 (2012)
- Kwon H, "Generation of regulatory dendritic cells and CD4+ Foxp3+ T cells by probiotics administration suppresses immune disorders", Proc. Natl. Acad. Sci. USA, 107, pp.2159~2164 (2010)
- 이정민, "Effect of Orally Administered Lactobacillus brevis HY7401 in a Food Allergy Mouse Model", Journal of Microbiology and Biotechnology, 23(11), pp.1636~1640 (2013), 한국미생물·생명공학회[DOI]
- Lutz MB, "Immature, semi-mature and fully mature dendritic cells: which signals induce tolerance or immunity?", Trends Immunol, 23, pp.445~449 (2002)
- Lyons A, "Bacterial strain-specific induction of Foxp3+ T regulatory cells is protective in murine allergy models", Clin. Exp. Allergy, 40, pp.811~819 (2010)
- Meulenbroek LA, "Oral treatment with β-lactoglobulin peptides prevents clinical symptoms in a mouse model for cow’s milk allergy", Pediat. Allerg. Immunol, 24, pp.656~664 (2013)
- Ross AC, "Vitamin A and retinoic acid in T cell–related immunity", Am. J. Clin. Nutr, 96, pp.1166S~1172S (2012)
- Schwarzer M, "Neonatal colonization of germ-free mice with Bifidobacterium longum prevents allergic sensitization to major birch pollen allergen Bet v 1", Vaccine, 46, pp.5405~5412 (2013)
- Shriver SK, "Thermal and nonthermal methods for food allergen control", Food Eng. Rev, 3, pp.26~43 (2011)
- Shurin MR, "Regulatory dendritic cells", Cancer Biol. Ther, 11, pp.988~992 (2011)
- Smit JJ, "The role of intestinal dendritic cells subsets in the establishment of food allergy", Clin. Exp. Allergy, 41, pp.890~898 (2011)
- So J, "Lactobacillus casei potentiates induction of oral tolerance in experimental arthritis", Mol. Immunol, 46, pp.172~180 (2008)
- Staden U, "Specific oral tolerance induction in food allergy in children: efficacy and clinical patterns of reaction", Allergy, 62, pp.1261~1269 (2007)
- Stern A, "Neonatal mucosal immune stimulation by microbial superantigen improves the tolerogenic capacity of CD103+ dendritic cells", PLoS One, 8, pp.e75594~ (2013)
- Thang CL, "Effects of Lactobacillus rhamnosus GG supplementation on cow’s milk allergy in a mouse model", Allergy Asthma Clin. Immunol, 7, pp.20~28 (2011)
- Wang XX, "Preventive effects of Lactobacillus casei-14 on ulcerative colitis in mice", J. Gansu Agric. Univ, 48, pp.1~5 (2013)
- You J, "Probiotic modulation of dendritic cell function is influenced by ageing", Immunobiology, 219, pp.138~148 (2014)
- Zhou XD, "Laxative effect of lactobacillus paracasei subsp. paracasei LC-01 in constipated mice", J. Dairy Sci. Technol, 35, pp.7~11 (2012)