Enhanced Production of Epothilone by Immobilized Sorangium cellulosum in Porous Ceramics
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초록
Epothilone, which is produced by the myxobacterium Sorangium cellulosum, contributes significant value in medicinal development. However, under submerged culture conditions, S. cellulosum will accumulate to form bacterial clumps, which hinder nutrient and metabolite transportation. Therefore, the production of epothilone by liquid fermentation is limited. In this study, diatomite-based porous ceramics were made from diatomite, paraffin, and poremaking agent (saw dust). Appropriate methods to modify the porous ceramics were also identified. After optimizing the preparation and modification conditions, we determined the optimal prescription to prepare high-performance porous ceramics. The structure of porous ceramics can provide a solid surface area where S. cellulosum can grow and metabolize to prevent the formation of bacterial clumps. S. cellulosum cells that do not form clumps will change their erratic metabolic behavior under submerged culture conditions. As a result, the unstable production of epothilone by this strain can be changed in the fermentation process, and the purpose of increasing epothilone production can be achieved. After 8 days of fermentation under optimized conditions, the epothilone yield reached 90.2 mg/l, which was increased four times compared with the fermentation without porous ceramics.
Abstract
Epothilone, which is produced by the myxobacterium Sorangium cellulosum, contributes significant value in medicinal development. However, under submerged culture conditions, S. cellulosum will accumulate to form bacterial clumps, which hinder nutrient and metabolite transportation. Therefore, the production of epothilone by liquid fermentation is limited. In this study, diatomite-based porous ceramics were made from diatomite, paraffin, and poremaking agent (saw dust). Appropriate methods to modify the porous ceramics were also identified. After optimizing the preparation and modification conditions, we determined the optimal prescription to prepare high-performance porous ceramics. The structure of porous ceramics can provide a solid surface area where S. cellulosum can grow and metabolize to prevent the formation of bacterial clumps. S. cellulosum cells that do not form clumps will change their erratic metabolic behavior under submerged culture conditions. As a result, the unstable production of epothilone by this strain can be changed in the fermentation process, and the purpose of increasing epothilone production can be achieved. After 8 days of fermentation under optimized conditions, the epothilone yield reached 90.2 mg/l, which was increased four times compared with the fermentation without porous ceramics.
참고문헌 (27)
- Abbott SL, "Biochemical properties of a newly described Escherichia species, Escherichia albertii", J. Clin. Microbiol, 41, pp.4852~4854 (2003)
- Almeida C, "Use of two different carriers in a packed bed reactor for endopolygalacturonase production by a yeast strain", Process Biochem, 40, pp.1937~1942 (2004)
- Berengut D., "Statisties for experiments: design, innovation, and discovery", Am. Stat, 60, pp.341~342 (2006)
- Davies J, "Introducing the parvome: bioactive compounds in the microbial world", ACS Chem. Biol, 7, pp.252~259 (2012)
- Devi S, "Optimization of critical parameters for immobilization of Streptomyces clavuligerus on alginate gel matrix for cephamycin C production", World J. Microbiol. Biotechnol, 15, pp.185~192 (1999)
- Diez J, "Myxobacteria: natural pharmaceutical factories", Microb. Cell Fact, 11, pp.52~54 (2012)
- Duarte JC, "Effect of immobilized cells in calcium alginate beads in alcoholic fermentation", AMB Express, 3, pp.31~ (2013)
- Forli S., "Epothilones: from discovery to clinical trials", Curr. Top Med. Chem, 14, pp.2312~2321 (2014)
- Gerth K, "Epothilone A and B: antifungal and cytotoxic compounds from Sorangium cellusosum (Myxobacteria)", J. Antibiot, 49, pp.560~563 (1996)
- Gong GL, "Preparation of diatomitebased porous ceramics for adsorbing and fixing Sorangium cellulosum", Modern Chem. Ind, 33, pp.66~70 (2013)
- Gong GL, "Genome shuffling improving the production of epothilone B", Chin. J. Antibiot, 38, pp.106~110 (2013)
- Gong GL, "Preparation and adsorption properties of mixed-templates molecularly imprinted polymers of epothilone B", J. Chem. Pharm. Res, 6, pp.1421~1427 (2014)
- Julieb B, "Heterologous expression of epothilone biosynthesis genes in Myxococcus xanthus", Antimicrob. Agents Chemother, 46, pp.2772~2778 (2002)
- Lau J, "Optimizing the heterologous production of epothilone D in Myxococcus xanthus", Biotechnol. Bioeng, 78, pp.280~286 (2002)
- Li YZ, "Mutation and a high-throughput screening method for improving the production of epothilone of Soragium", J. Ind. Microbiol. Biotechnol, 34, pp.615~623 (2007)
- Li ZF, "Evolutionary diversity of ketoacyl synthases in cellulolytic myxobacterium Sorangium", Syst. Appl. Microbiol, 30, pp.189~196 (2007)
- Manikandan R, "Studies on degradation of chlorinated aromatic hydrocarbon by using immobilized cell crude extract of Pseudomonas aeruginosa", Afr. J. Biotechnol, 6, pp.1338~1342 (2007)
- McMeen CR, "NOM removal by slow sand filtration through iron oxide-coated olivine", J. Am. Water Works Assoc, 89, pp.57~71 (1997)
- Noworyta A, "Process of penicillin G hydrolysis catalyzed by penicillin acylase immobilized on acylic carrier", Bioproc. Eng, 9, pp.271~275 (1993)
- Regentin R, "Nutrient regulation of epothilone biosynthesis in heterologous and native production", Appl. Microbiol. Biotechnol, 61, pp.451~455 (2003)
- Reichenbach H, "The Prokaryotes, Vol 2", pp.3416~3487 (1992), Springer
- Rivera E, "Clinical development of ixabepilone and other epothilone in patients with advanced solid tumors", Oncologist, 13, pp.1207~1223 (2008)
- Roche H, "Phase clinical trial of ixabepilone (BMS-247550), an epothilone B analog, as first-line therapy in patients with metastatic breast cancer previously treated with anthracycline chemotherapy", J. Clin. Oncol, 25, pp.3415~3420 (2007)
- Sim SJ, "Improvement of epothilone B production by in situ removal of ammonium using cation exchange resin in Sorangium cellulosum culture", Biochem. Eng. J, 37, pp.328~331 (2007)
- PARK, SANG WOO, "Repeated Batch Production of Epothilone B by Immobilized Sorangiumcellulosum", Journal of Microbiology and Biotechnology, 17(7), pp.1208~1212 (2007), 한국미생물·생명공학회
- Veelken M, "Production of tylosin and nikkomycin by immobilized Streptomyces cells", Appl. Microbiol. Biotechnol, 15, pp.206~210 (1982)
- Zhang XB, "Preparation and characterization of porous cordierte ceramics from fly ash", J. Coal Sci. Eng, 34, pp.247~251 (2007)